Quantification and Localization of Watermelon Chlorotic Stunt Virus and Tomato Yellow Leaf Curl Virus (Geminiviridae) in Populations of Bemisia tabaci (Hemiptera, Aleyrodidae) with Differential Virus Transmission Characteristics

Kollenberg, Mario; Winter, Stephan; Götz, Monika

doi:10.1371/journal.pone.0111968

Cited by 26 publications

(20 citation statements)

References 80 publications

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“…This suggested that TYLCV acquisition is a function of virus accumulation on the plant tissue they feed on. Kollenberg et al recently proved this hypothesis by exposing whiteflies to feed from a solution of purified virions at a specific concentration and thereafter quantifying virus load in the insects [ 45 ]. In addition, previous findings also revealed that whiteflies were able to acquire TYLCV from infected resistant genotypes and inoculate the same to non-infected hosts [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Temporal Effects of a Begomovirus Infection and Host Plant Resistance on the Preference and Development of an Insect Vector, Bemisia tabaci, and Implications for Epidemics

et al. 2015

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Persistent plant viruses, by altering phenotypic and physiological traits of their hosts, could modulate the host preference and fitness of hemipteran vectors. A majority of such modulations increase vector preference for virus-infected plants and improve vector fitness, ultimately favouring virus spread. Nevertheless, it remains unclear how these virus-induced modulations on vectors vary temporally, and whether host resistance to the pathogen influences such effects. This study addressed the two questions using a Begomovirus-whitefly-tomato model pathosystem. Tomato yellow leaf curl virus (TYLCV) -susceptible and TYLCV-resistant tomato genotypes were evaluated by whitefly-mediated transmission assays. Quantitative PCR revealed that virus accumulation decreased after an initial spike in all genotypes. TYLCV accumulation was less in resistant than in susceptible genotypes at 3, 6, and 12 weeks post inoculation (WPI). TYLCV acquisition by whiteflies over time from resistant and susceptible genotypes was also consistent with virus accumulation in the host plant. Furthermore, preference assays indicated that non-viruliferous whiteflies preferred virus-infected plants, whereas viruliferous whiteflies preferred non-infected plants. However, this effect was prominent only with the susceptible genotype at 6 WPI. The development of whiteflies on non-infected susceptible and resistant genotypes was not significantly different. However, developmental time was reduced when a susceptible genotype was infected with TYLCV. Together, these results suggest that vector preference and development could be affected by the timing of infection and by host resistance. These effects could play a crucial role in TYLCV epidemics.

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Section: Discussionmentioning

confidence: 99%

Temporal Effects of a Begomovirus Infection and Host Plant Resistance on the Preference and Development of an Insect Vector, Bemisia tabaci, and Implications for Epidemics

et al. 2015

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“…Tomato yellow leaf curl virus and other begomoviruses are exclusively transmitted by the B. tabaci in a persistent-circulative manner, such that once ingested by an adult whitefly, virions pass to the midgut, where they move across the midgut membrane to the hemolymph [ 5 , 35 ], and circulate back into the primary salivary glands, where they are egested with saliva during insect feeding [ 7 , 36 , 37 ]. By analyzing miRNA expression in the present study, and in reference to the relevant mRNA expression from our previous study [ 13 ], we gained insight into global gene expression and regulation of whitefly genes potentially associated with TYLCV transmission.…”

Section: Discussionmentioning

confidence: 99%

Deep Sequencing of Small RNAs in the Whitefly Bemisia tabaci Reveals Novel MicroRNAs Potentially Associated with Begomovirus Acquisition and Transmission

Hasegawa

Shamimuzzaman

Chen

et al. 2020

Insects

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The whitefly Bemisia tabaci (Gennadius) is a notorious insect vector that transmits hundreds of plant viruses, affecting food and fiber crops worldwide, and results in the equivalent of billions of U.S. dollars in crop loss annually. To gain a better understanding of the mechanism in virus transmission, we conducted deep sequencing of small RNAs on the whitefly B. tabaci MEAM1 (Middle East-Asia Minor 1) that fed on tomato plants infected with tomato yellow leaf curl virus (TYLCV). Overall, 160 miRNAs were identified, 66 of which were conserved and 94 were B. tabaci-specific. Among the B. tabaci-specific miRNAs, 67 were newly described in the present study. Two miRNAs, with predicted targets encoding a nuclear receptor (Bta05482) and a very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 (Bta10702), respectively, were differentially expressed in whiteflies that fed on TYLCV-infected versus uninfected plants. To better understand the regulatory effects of identified miRNAs and their target genes, we correlated expression profiles of miRNAs and their target transcripts and found that, interestingly, miRNA expression was inversely correlated with the expression of ~50% of the predicted target genes. These analyses could serve as a model to study gene regulation in other systems involving arthropod transmission of viruses to plants and animals.

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“…However, the virus content in the source plants was not estimated in this study; such an estimate might provide some insight into the variability in the SRBSDV and RBSDV GEC in the respective insect species. Virus level in source plants has also been shown to affect the concentration of watermelon chlorotic stunt virus (WCSV) and tomato yellow leaf curl virus (TYLCV) in whitefly vectors (Kollenberg et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time

et al. 2015

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Quantification and Localization of Watermelon Chlorotic Stunt Virus and Tomato Yellow Leaf Curl Virus (Geminiviridae) in Populations of Bemisia tabaci (Hemiptera, Aleyrodidae) with Differential Virus Transmission Characteristics

Cited by 26 publications

References 80 publications

Temporal Effects of a Begomovirus Infection and Host Plant Resistance on the Preference and Development of an Insect Vector, Bemisia tabaci, and Implications for Epidemics

Temporal Effects of a Begomovirus Infection and Host Plant Resistance on the Preference and Development of an Insect Vector, Bemisia tabaci, and Implications for Epidemics

Deep Sequencing of Small RNAs in the Whitefly Bemisia tabaci Reveals Novel MicroRNAs Potentially Associated with Begomovirus Acquisition and Transmission

Quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time

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