Quantificação simultânea de indicadores biológicos de exposição a solventes orgânicos por cromatografia líquida de alta eficiência

Bulcão, Rachel Picada; Maria, Lucas Santa; Charão, Mariele Feiffer; Moro, Ângela M.; Roehrs, Miguel; Garcia, Solange Cristina; Limberger, Renata Pereira

doi:10.1590/s0100-40422008000600013

Cited by 9 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantification of a urinary metabolite of benzene (trans,transmuconic acid-t,t-MA) was carried out by high-performance liquid chromatography with UV detection (HPLC-UV; Shimadzu, USA) after solid phase extraction (SPE) according to a previously described analytical method (Ducos et al, 1990), with modifications (SPE cartridge: 100 mg; sample volume: 200 mL). Quantification of urinary metabolites of toluene (hippuric acid-HA) and xylene (methylhippuric acid-mHA) was simultaneously performed by HPLC-UV (Bulcão et al, 2008). The creatinine concentration was measured by spectrophotometry as previously described (Jaffé, 1886) using commercial kits (Doles reagents, Brazil).…”

Section: Exposure Assessmentmentioning

confidence: 99%

Early hematological and immunological alterations in gasoline station attendants exposed to benzene

Moro

Brucker

Charão

et al. 2015

Environmental Research

View full text Add to dashboard Cite

Section: Exposure Assessmentmentioning

confidence: 99%

Early hematological and immunological alterations in gasoline station attendants exposed to benzene

Moro

Brucker

Charão

et al. 2015

Environmental Research

View full text Add to dashboard Cite

“…Several methods have been developed to quantify HA and 4‐MHA in urine samples using gas chromatography as well as liquid chromatography . Besides, only two papers were published about their quantifications using capillary electrophoresis (CE) , despite the advantages this technique presents, like high analytical frequency and low sample and reagent consumptions .…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted probe for solid‐phase extraction of hippuric and 4‐methylhippuric acids directly from human urine samples followed by MEKC analysis

et al. 2017

View full text Add to dashboard Cite

Hippuric acid (HA) and 4-methylhippuric acid (4-MHA) are metabolites as well as biological indicators for toluene and xylenes, respectively, and their determination in urine samples is very important, in order to monitor the occupational exposition to these solvents, ensuring a safe working environment. Thus, this paper describes the synthesis and characterization of a probe impregnated with molecularly imprinted polymers (MIPs) for the solid-phase extraction of HA and 4-MHA directly from untreated urine samples followed by micellar electrokinetic chromatography (MEKC) analyses. The MIP probe selectivity was compared to the non-imprinted polymer probe. The MEKC separations were carried out in 50 mmol/L sodium tetraborate pH 10.0/0.5 mmol/L cetyltrimethylammonium bromide aqueous solution, with a constant voltage of -15 kV. The system variables were optimized to provide ideal conditions for the extraction and desorption of the analytes, as well as for the MEKC analyses. The method was linear from 0.5 to 5.0 g/L for both analytes, with correlation coefficients > 0.994. Precisions and accuracies, expressed as relative standard deviation and relative error, were < 20.0 and within -15.4 to 16.6%, respectively, in accordance with the United States Food and Drug Administration recommendation. The MIP probe has proven to be simple, cheap, resistant, and synthetically reproducible, being successfully used to analyze both HA and 4-MHA from real samples.

show abstract

Environmental exposure and effects on health of children from a tobacco-producing region

Nascimento

Göethel

Baierle

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

Children may be environmentally exposed to several hazards. In order to evaluate the health of children living in a tobacco-producing region, different biomarkers of exposure and effect, as well as hematological parameters, were evaluated. Biomarkers of exposure to the following xenobiotics were assessed: pesticides, nicotine, toxic elements, and organic solvents. Oxidative damage markers malondialdehyde (MDA) and protein carbonyls (PCO), vitamin C, microalbuminuria (mALB) levels, and N-acetyl-β-D-glucosaminidase (NAG) activity were also evaluated. Peripheral blood samples and urine were collected from 40 children (6-12 years), at two different crop periods: in the beginning of pesticide applications (period 1) and in the leaf harvest (period 2). The Wilcoxon signed-rank test for paired data was used to evaluate the differences between both periods. Biomarkers of exposure cotinine in urine and blood chromium (Cr) levels were increased in period 1 when compared to period 2. Moreover, a significant reduced plasmatic activity of butyrylcholinesterase (BuChE) was observed in period 2 in relation to period 1. Blood Cr levels were above the recommended by WHO in both evaluations. The biomarkers MDA and PCO as well as the kidney dysfunction biomarker, mALB, presented levels significantly increased in period 1. Additionally, decreased lymphocytes and increased basophils were also observed. Cotinine was positively associated with PCO, and Cr was positively associated with PCO and MDA. The increased Cr levels were associated with decreased lymphocytes and increased basophils. Our findings demonstrate that children environmentally exposed to xenobiotics in rural area may present early kidney dysfunction, hematological alterations, as well as lipid and protein damages, associated with co-exposure to different xenobiotics involved in tobacco cultivation.

show abstract

Quantificação simultânea de indicadores biológicos de exposição a solventes orgânicos por cromatografia líquida de alta eficiência

Cited by 9 publications

References 9 publications

Early hematological and immunological alterations in gasoline station attendants exposed to benzene

Early hematological and immunological alterations in gasoline station attendants exposed to benzene

Molecularly imprinted probe for solid‐phase extraction of hippuric and 4‐methylhippuric acids directly from human urine samples followed by MEKC analysis

Environmental exposure and effects on health of children from a tobacco-producing region

Contact Info

Product

Resources

About