Quantificação de fenóis totais presentes nas cascas de Trichillia catigua A. Juss (Meliaceae)

Rabelo, D.; Paula, José Realino de; Bara, Maria Teresa Freitas

doi:10.1590/s1516-05722013000200010

Cited by 4 publications

(4 citation statements)

References 24 publications

(22 reference statements)

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“…The phytochemical composition of Trichilia catigua barks is well described in the literature. The flavalignans (flavanols substituted with phenylpropanoids) cinchonains IIa, Ia and Ib and proanthocyanidins were isolated in extracts obtained from barks of T. catigua [ 8 – 10 ]. In our study, we intended to identify the compounds extracted from the barks of T. catigua with different solvents in order to correlate the phytochemical profile with the in vitro activities.…”

Section: Discussionmentioning

confidence: 99%

“…Flavonoids, tannins, alkaloids, saponins, among other phytochemical classes were identified in the barks of T. catigua [ 4 ]. The bark contains high concentrations of polyphenols including flavan-3-ols (procyanidin B2, epicatechin, catechin), flavalignans (cinchonains Ia, Ib, IIa, IIb) and phenylpropanoid derivatives (chlorogenic acid) [ 7 – 10 ]. The main constituents of T. catigua exhibited potent antioxidant activity, which is important in the prevention of cellular damage triggered by oxidative stress in acute and chronic neuropathological conditions [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant, anticholinesterase and antifatigue effects of Trichilia catigua (catuaba)

Martins¹,

Brito²,

Araújo³

et al. 2018

BMC Complement Altern Med

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BackgroundTrichilia catigua A. Juss. (Meliaceae) is a species known as catuaba and used in folk medicine for the treatment of fatigue, stress, impotence and memory deficit. The main phytochemical compounds identified in the barks of T. catigua are flavalignans, flavan-3-ols and flavonoids which are associated with its antioxidant activity. Pre-clinical studies with T. catigua extracts have identified many pharmacological properties, such as anti-inflammatory, antidepressant, antinociceptive, pro-memory and neuroprotective against ischemia and oxidative stress. This study was designed in order to compare the chemical composition and in vitro antioxidant and anticholinesterase activity of four different polarity extracts and selected the one most active for in vivo studies in rodent models of stress, fatigue and memory.MethodsHexane, chloroform, hydroalcoholic and aqueous extracts from bark of Trichilia catigua were analyzed by RPHPLC-DAD-ESI-MS/MS. Antioxidant activity was assessed by 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay and acetylcholinesterase inhibition by Ellman’s modified method. In vivo studies (stress, fatigue and memory) were carried out with adult male mice and rats treated with hydroalcoholic extract in doses of 25–300 mg/kg (p.o.).ResultsWe confirmed the presence of cinchonain IIa, Ia and Ib, as main constituents in the four extracts, while procyanidins were detected only in hydroalcoholic extract. Antioxidant and anticholinesterase activity were observed for all extracts, with most potent activity found on the hydroalcoholic extract (EC50 = 43 μg/mL and IC50 = 142 μg/mL for DPPH scavenger and acetylcholinesterase inhibition, respectively). The treatment of laboratory animals with hydroalcoholic extract did not protect rats from cold immobilization stress and did not prevent the scopolamine-induced amnesia in mice. However, the treatment of mice with the hydroalcoholic extract partially reduced the fatigue induced by treadmill, since the highest dose increased the spontaneous locomotor activity and reduced the deficit on grip strength after the forced exercise (p < 0.05), in some observation times.ConclusionsThese data suggest the hydroalcoholic extract as the most suitable for plant extraction and partially support the folk use of T. catigua as antifatigue drug.Graphical abstract.Trichilia catigua hydroalcoholic extract exhibits antioxidant and anticholinesterase activity in vitro and reduces the fatigue induced by forced exercise.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2222-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidant, anticholinesterase and antifatigue effects of Trichilia catigua (catuaba)

Martins¹,

Brito²,

Araújo³

et al. 2018

BMC Complement Altern Med

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“…Much research has revealed that phenolic compounds are mainly responsible for the antioxidant activity in fruits [ 53 , 58 , 59 , 60 , 61 , 62 ]. The antioxidant activity was performed by the free radical scavenging ABTS and free radical scavenging DPPH, and the results are shown in Table 4 .…”

Section: Resultsmentioning

confidence: 99%

Microencapsulation by Spray Drying and Antioxidant Activity of Phenolic Compounds from Tucuma Coproduct (Astrocaryum vulgare Mart.) Almonds

et al. 2022

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The industrial processing of fruits in the Amazon region, such as tucuma, generates a large amount of coproducts with great nutritional potential. In this work, phenolic compounds from tucuma coproduct almonds were extracted and microencapsulated by spray drying using maltodextrin as the encapsulating agent and verified its antioxidant activity. Phenolic compounds were determined by UV spectroscopy and identified by Ultraefficiency Liquid Chromatography. Antioxidant activity was measured by ABTS and DPPH assay. Thermogravimetric techniques, infrared spectroscopy, scanning electron microscopy, moisture content and water activity were applied to characterize the microparticle. The crude extract and microparticle had total polyphenols of 135.1 mg/g ± 0.078 and 130.5 mg/g ± 0.024, respectively. Caffeic and gallic acids were identified. The crude extract and the microparticle showed good antioxidant activity by ABTS and DPPH assay, justified by the presence of the phenolic compounds found. The microparticle showed spherical and heterogeneous structures and good encapsulation efficiency from the spray drying process using maltodextrin. The results show that the extract of the tucuma almond coproduct can be used as a phenolic compound-rich source and microencapsulated with possible application for functional food production.

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“…Estes resultados evidenciam compatibilidade com este estudo, apesar da utilização de equipamentos diferentes, as metodologias apresentamótimos resultados dos parâmetros de validação. Rabelo, Paula e Bara [30] validaram o método espectrofotométrico para determinação do teor de fenóis totais da Trichillia. catigua A. Juss (Meliaceae) e comprovaram que esté e seletivo, linear, preciso, exato e robusto, apresentado resultados de 95% de exatidão o qué e coerente com o trabalho estudado.…”

Section: Tabela 2 Precisão Intermediária Para Amostras Desengorduradasunclassified

Spectrophotometric method validation for detection of phenolic compounds of Spirulina sp. LEB-18

Christ-Ribeiro¹,

Goularte²,

Souza-Soares³

2017

Revista Ciencias Exatas E Naturais

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Resumo: O uso de bioativos provenientes de fontes naturais requer a extração e purificação destes compostos para aplicação em aditivos e ingredientes alimentares, farmacêuticos e cosméticos. Este trabalho teve por objetivo validar a quantificação de compostos fenólicos a partir da microalga Spirulina sp. LEB-18. A validação da metodologia seguiu o Instituto Nacional de Metrologia, Normalização e Qualidade Industrial (INMETRO) e International Conference on Harmonisation (ICH), sendo avaliados os parâmetros de especificidade, linearidade, robustez, limite de detecção, limite de quantificação, precisão e exatidão. O método apresentou coeficiente de correlação de 0,9978, confirmando a linearidade do método. Nas análises de precisão intermediária para amostras desengorduradas não houve diferença significativa (p < 0, 05) entre analistas e o desvio padrão relativo não ultrapassou de 2%, assim como na análise de reprodutividade de amostras in natura integral e desengordurada. Na análise de exatidão observou-se boa recuperação, dentro da faixa de 80 a 120%. A validação da metodologia analítica demonstrou-se apropriada para determinar a concentração de compostos fenólicos em extratos de Spirulina sp. LEB-18.Palavras-chave: compostos fenólicos; linearidade; microalga; validação analítica.Abstract: The use of bioactive compounds in the preparation of food additives and ingredients as well as pharmaceuticals and cosmetics, requires the extraction and purification of these compounds from natural sources. This study aimed to validate the quantification of phenolic compounds in Spirulina sp. LEB-18 samples. The validation of the methodology followed INMETRO and ICH, and evaluated the specific parameters, linearity, ruggedness, detection limit, quantification limit, precision and accuracy. The method presented correlation coefficient of 0.9978, confirming the linearity of the method. In the intermediate precision analysis to degreased samples there was no significant difference (p < 0, 05) between analysts and the relative standard deviation did not exceed 2%, as well as the reproducibility of analysis of samples in natura full and degreased. For accurate analysis good recovery was

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Quantificação de fenóis totais presentes nas cascas de Trichillia catigua A. Juss (Meliaceae)

Cited by 4 publications

References 24 publications

Antioxidant, anticholinesterase and antifatigue effects of Trichilia catigua (catuaba)

Antioxidant, anticholinesterase and antifatigue effects of Trichilia catigua (catuaba)

Microencapsulation by Spray Drying and Antioxidant Activity of Phenolic Compounds from Tucuma Coproduct (Astrocaryum vulgare Mart.) Almonds

Spectrophotometric method validation for detection of phenolic compounds of Spirulina sp. LEB-18

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