Quantal and graded dose-responses of bluetongue virus: a comparison of their sensitivity as assay methods for neutralizing antibody

Svehag, Sven‐Eric

doi:10.1017/s0022172400040493

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…The serum-virus neutralisation (VN) assay (or variants such as plaque neutralisation) is used to serotype virus isolates and for quantitation of antibodies against specific BTV serotypes (16,63,72,73). Interpreting antibody responses against specific serotypes can be problematic, particularly where animals have been infected with more than one serotype.…”

Section: Serological Assaysmentioning

confidence: 99%

Diagnostic applications of molecular and serological assays for bluetongue and African horse sickness

Mayo¹,

Weyer²,

Carpenter³

et al. 2021

Rev. Sci. Tech. OIE

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The availability of rapid, highly sensitive and specific molecular and serological diagnostic assays, such as competitive enzyme-linked immunosorbent assay (cELISA), has expedited the diagnosis of emerging transboundary animal diseases, including bluetongue (BT) and African horse sickness (AHS), and facilitated more thorough characterisation of their epidemiology. The development of assays based on real-time, reverse-transcription polymerase chain reaction (RT-PCR) to detect and identify the numerous serotypes of BT virus (BTV) and AHS virus (AHSV) has aided in-depth studies of the epidemiology of BTV infection in California and AHSV infection in South Africa. The subsequent evaluation of pan-serotype, real-time, RT-PCR-positive samples through the use of serotype-specific RT-PCR assays allows the rapid identification of virus serotypes, reducing the need for expensive and time-consuming conventional methods, such as virus isolation and serotypespecific virus neutralisation assays. These molecular assays and cELISA platforms provide tools that have enhanced epidemiological surveillance strategies and improved our understanding of potentially altered Culicoides midge behaviour when infected with BTV. They have also supported the detection of subclinical AHSV infection of vaccinated horses in South Africa. Moreover, in conjunction with whole genome sequence analysis, these tests have clarified that the mechanism behind recent outbreaks of AHS in the AHS-controlled area of South Africa was the result of the reversion to virulence and/or genome reassortment of live attenuated vaccine viruses. This review focuses on the use of contemporary molecular diagnostic assays in the context of recent epidemiological studies and explores their advantages over historic virus isolation and serological techniques.

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Section: Serological Assaysmentioning

confidence: 99%