Quality Indicators of Semen of Roosters of Three Parental Layer Lines and Specific Conductivity of the Semen

Abstract

Máchal L., I. Kfiivánek: Indicators of Semen Quality of Roosters of Three Parental LayerLines and Specific Conductivity of the Semen. Acta Vet. Brno 71, 2002: 109-116. Relations between semen quality and the specific conductivity of the semen was determined in 90 clinically healthy roosters of three parental lines (BPR -04, RIW -06 and RIR -05) in a breeding flock of the Moravia laying hybrids. At the age of 143, 205 and 240 days the volume, sperm concentration and sperm motility was measured in the ejaculates and at the age of 205 and 240 days also the specific conductivity. The average volume of the ejaculate of the cocks of all three parental lines ranged between 0.20 cm 3 and 0.62 cm 3 . On the 143 rd day of age the average volume of the ejaculate was the lowest (between 0.20 cm 3 and 0.30 cm 3 ), and was the highest on the 240 th day (between 0.51 cm 3 and 0.62 cm 3 ). The average sperm motility of the ejaculate was similar; at the beginning of the investigations, i.e. on day 143 of age, it ranged between 31.7% and 54.1%, at the age of 240 days it was higher (66.8% -86.2%). The average sperm concentration was balanced, i.e. between 0.75 ⋅ 10 9 cm -3 and 1.06 ⋅ 10 9 cm -3 . The average total number of sperm (TNS) in the ejaculate and the average total number of motile sperm in the ejaculate of the cocks (TNMS) increased with the age of the cocks. The average TNS increased from the initial 0.16 ⋅ 10 9 -0.32 ⋅ 10 9 on day143 of age to 0.38 ⋅ 10 9 -0.59 ⋅ 10 9 on day 240. The average TNMS also increased from the initial 0.09 ⋅ 10 9 -0.16 ⋅ 10 9 on day143 of age to 0.26 ⋅ 10 9 -0.51 ⋅ 10 9 on day 240. In terms of inter-line difference the lowest average TNMS was reported in cocks of the RIR -05 universal paternal line (between 0.09 ⋅ 10 9 and 0.26 ⋅ 10 9 ) and the highest in cocks of the BPR -04 paternal line (between 0.09 ⋅ 10 9 and 0.51 ⋅ 10 9 ).The average specific conductivity of the ejaculate was measured with four electrodes and ranged between 0.84 S ⋅m -1 and 1.02 S ⋅ m -1 . No marked differences in the average values of the specific conductivity were observed among cocks of the respective lines, but the lowest average specific conductivity was discovered in the ejaculates of cocks with the lowest number of motile sperms (line RIW -06 on the 205 th day of age, i.e. 1.02 S ⋅ m -1 ). Negative correlations were found between sperm concentration and motility and the specific conductivity of the ejaculate in cocks of all the three paternal lines of the domestic fowl (r p = -0.15 to -0.45). The calculated correlations, particularly those that were statistically highly significant or significant (between sperm concentration, sperm motility and specific conductivity of the ejaculate) demonstrated that these properties could be used for complementary examinations of the ejaculate quality. Roosters, sperm, ejaculate volume, sperm concentration, sperm motility, conductivityThe qualitative parameters of the ejaculates of roosters are considerably variable. In cocks of the paternal and maternal lines ·teÀo...

Physical properties of stallion semen in relation to some qualitative and quantitative characteristics

“…Not a great number of scientists studied physical properties of semen (Garnica et al, 1993;Bravo et al, 2000;Máchal and Křivánek, 2002;Andrade-Rocha, 2005;Esfandiari et al, 2006). Máchal and Křivánek (2000) (Máchal and Křivánek, 2002).…”

“…The sperm concentration in the ejaculate was determined hematocytometrically, by counting cells in Bürker chambers. The electrical conductivity of semen was assessed according to the methodology by Máchal and Křivánek (2002). It was measured by bioimpedance spectroscopy analyser, which allows measuring electrical conductivity as a function of frequency in the range of 10 Hz to 100 kHz.…”

“…Viscosity is a typical characteristic of a real fl uid. It arises from the shear stress between the layers of the fl uid fl ow (Máchal et al, 1977;Máchal and Křivánek, 2002). The issue of the electrical conductivity and dynamic viscosity were rarely studied (Garnica et al, 1993;Bravo et al, 2000;Andrade-Rocha, 2005;Esfandiari et al, 2006).…”

Environmentální gramotnost učitelů a studentů učitelství

“…Dlouhá (2012) definuje environmentální gramotnost jako: "proces poznávání a osvojování si takových způsobů myšlení, které vedou k porozumění souvislostem a vztahům uvnitř i vně lidských společenství a přírodních systémů." Environmentální gramotnost můžeme také chápat jako reakci jedince (podmíněnou schopnostmi, dovednostmi, postoji a hodnotami) na každodenní situace, které se týkají životního prostředí (Máchal 2000). Důležitou roli přitom hraje i osobní vztah člověka k přírodě.…”

Environmentální výchova na prvním stupni základních škol

“…Teprve v 80. a 90. letech dvacátého století vznikají ucelenější pokusy environmentální výchovu nějak vymezit oproti environmentalistice, ekologii a společenským vědám a specifikovat jak její obsah, tak cíle a prostředky. Následovalo vydání publikací Špetka dobromysli (Máchal, 1996), Průvodce praktickou ekologickou výchovou (Máchal, 2000), Desatero domácí ekologie (Máchal aj., 2001), Ekologická výchova a vzdělávání ve škole 21. století (Horká, 2005) a dosud poslední Činčerova Environmentální výchova : od cílů k prostředkům (Činčera, 2007).…”