Quality Improvement of Boar Seminal Doses

Bussalleu, E; Torner, Eva

doi:10.1007/978-3-642-35049-8_10

Cited by 7 publications

(22 citation statements)

References 100 publications

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“…In general, it is known that the semen collection process is far from being a sterile procedure because of the involvement of multiple sources that can lead to bacterial contamination (Bussalleu and Torner, 2013). In this sense, additional measures such as regular monitoring of the animals and semen, biosafety measures to reduce contamination during collection, processing and storage, and the treatment of semen with the appropriate antimicrobials (Maes et al, 2008) are necessary.…”

Section: Sources and Consequences Of Bacterial Contamination In Semenmentioning

confidence: 99%

Current and alternative trends in antibacterial agents used in mammalian semen technology

Santos¹,

Silva²

2020

Anim Reprod

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How to cite: Santos CS, Silva AR. Current and alternative trends in antibacterial agents used in mammalian semen technology. AbstractThe use of antibacterial substances as additives in extenders for ensuring the sanitary quality of the semen employed in reproductive biotechniques and preserving it from bacterial deterioration has been reported since the mid-twentieth century. However, the deleterious effects of these drugs on the sperm quality as well as their effectiveness in controlling bacterial growth in the preserved semen have been questioned. The aim of this review was to report the antimicrobials primarily used in the extenders added to the semen of mammals, and to present alternatives to their use. Among the various mammalian species, there is a large variation regarding the antimicrobial types added to semen extenders as cephalosporins (ceftiofur, cefdinir, eg) and quinolones (ofloxacin, ciprofloxacin), alone or in combination with large action spectra substances as penicillin-streptomycin and gentamicin-tylosin-lincomycin-spectinomycin. To combat problems related to bacterial resistance to these drugs, the emergence of alternatives is increasingly evident. Among these alternatives, use of physical methods as centrifugation and filtration, as well as the use of antimicrobial peptides and other substances from different origins have been highlighted for presenting antimicrobial potential.

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Section: Sources and Consequences Of Bacterial Contamination In Semenmentioning

confidence: 99%

Current and alternative trends in antibacterial agents used in mammalian semen technology

Santos¹,

Silva²

2020

Anim Reprod

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“…Studying the factors that may influence the biological value of semen subjected to preservation and artificial insemination (AI) is of great importance within the assisted-reproduction biotechnologies in pigs. Of these factors, an important role is attributed to the presence of microbial flora [ 1 , 2 , 3 , 4 ]. Changes of semen in terms of metabolism (glucose–sperm–germ relationship) may be caused by various germs types and their number per volume unit [ 1 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Ciornei¹,

Drugociu²,

Ciornei³

et al. 2021

Molecules

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The aim of the study was to establish the complete microbiological profile of boar semen (Sus scrofa domesticus) and to choose the most effective antiseptic measures in order to control and optimize AI reproduction in pig farms. One hundred and one semen samples were collected and analyzed from several pig farms. The microbiological profile of ejaculates was determined by evaluating the degree of contamination of fresh semen and after dilution with specific extenders. The bacterial and fungal load of fresh boar semen recorded an average value of 82.41/0.149 × 103 CFU/mL, while after diluting the ejaculates the contamination value was 0.354/0.140 × 103 CFU/mL. Twenty-four germs (15 bacterial and 9 fungal species) were isolated, the most common being Candida parapsilosis/sake (92%) and Escherichia coli (81.2%). Modification of the sperm collection protocol (HPBC) reduced contamination in raw sperm by 49.85% in bacteria (significant (p < 0.00001) and by 9.67% in fungi (non-significant (p < 0.111491). The load in bacteria and filamentous fungi can be controllable, but not in levuras fungi. Some fluconazole-added extenders (12.5 mg%), ensure fungal aseptization, and even an increase in sperm progressivity (8.39%) for at least a 12 h shelf life after dilution. Validation of sperm aseptization was done by maintaining sow fecundity unchanged after AI (insignificant p > 0.05).

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“…After the classical examinations and the determination of the volume and concentration, the dilution ratio was calculated / indicated automatically. For each insemination dose, 3.5 billion (3.5x10 9 ) sperm were assigned in a volume of 80 ml. Those that will be used to repeat AI were stored in incubators at a temperature of + 17˚C, up to 5 to 7 days, depending on the type of sperm extender, [36].…”

Section: Preparation Of Ai Doses and Their Preservationmentioning

confidence: 99%

“…Studying the factors that may influence the biological value of semen subjected to preservation and artificial insemination (AI) is of great importance within the assisted reproduction biotechnologies in pigs. Of these factors, an important role is attributed to the presence of microbial flora [5,9,12,28]. Changes of semen in terms of metabolism may be caused by various bacteria types and their number per volume unit [5,13].…”

Section: Introductionmentioning

confidence: 99%

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Ciornei¹,

Drugociu²,

Ciornei³

et al. 2021

Preprint

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The aim of the study is to establish the complete microbiological profile of boar semen and to choose the most effective antiseptic measures in order to control and optimize AI reproduction in pigs. More than one hundred semen samples were collected and analyzed from several pig farms. The microbiological profile of ejaculates was determined by determining the degree of con-tamination of fresh semen and after dilution with specific extenders. The bacterial and fungal load of fresh boar semen recorded an average value of 82.41/0.149x103CFU/mL, while after diluting the ejaculates the contamination value was 0.354/0.140 x103CFU/mL. 23 bacterial and fungal species were isolated, the most common being Candida parapsilosis/sake (92%), Escherichia coli (81.2%). Modification of the sperm collection protocol (HPBC) reduced contamination in raw sperm (by 49.85% in bacteria and by 9.67% in fungi). The load in bacteria and filamentous fungi can be con-trollable, but not in levuras fungi. In some Fluconazole-added extenders (12.5mg%), ensures the solution of this problem, and even increase in sperm progressivity (8.39%) for at least a 12-hour shelf life after dilution. The validation of the experiment was done by obtaining the sow fertility rate after AI.

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Quality Improvement of Boar Seminal Doses

Cited by 7 publications

References 100 publications

Current and alternative trends in antibacterial agents used in mammalian semen technology

Current and alternative trends in antibacterial agents used in mammalian semen technology

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

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