Quality evaluation for Radix Astragali based on fingerprint, indicative components selection and QAMS

Abstract

Radix Astragali (RA) is one of the most widely used Chinese herbs prescribed in many Chinese formulas to reinforce 'Qi' and treat vital energy deficiency. This study combined fingerprinting with quantitative analysis multi-components by a single marker (QAMS) to improve the quality control standard for RA on the basis of existing quality control methods of traditional Chinese medicinal materials. UPLC-ESI-TOF-MS technique was used to evaluate the quality of RA by fingerprinting and QAMS. Using the anti-inflammatory, anti-oxidation and anti-anoxic activities to screen characteristic components of RA, the calycosin-7-O-β-d-glucoside (CG), ononin, astragaloside IV, astragaloside II, calycosin and astrageloside I significantly inhibited ear edema in mice, the calycosin and CG had good antioxidant activity and the astragaloside I had a significant anti-hypoxia activity. Astragaloside I, astragaloside II, astragaloside IV, ononin, calycosin and CG had significant pharmacological actions. These components were comprehensively used as the indicative components for the quality control of RA. Astragaloside I was used as the internal standard of the relative correction factors of CG (13.45), ononin (0.51), calycosin (12.08), astragaloside IV (0.73) and astragaloside II (0.81). Astragaloside I and CG were used as internal standards of the relative correction factors of the flavonoids and saponins of ononin (1.11), calycosin (0.04), astragaloside IV (0.73) and astragaloside II (0.81). The study combined fingerprinting with QAMS to improve the quality control standard for RA.

Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker

“…Methods for calculating the RCFs have been previously reported [24,37]. First, gastrodin was selected as the internal standard, and a multipoint method (Equation (1)) was used to calculate the relative correction factors (RCF) for p -hydroxy benzaldehyde, p -hydroxybenzyl alcohol, parishin A, parishin B, parishin E, and parishin C. Then the content of the measured component was calculated according to Equation (2) [38].…”

Variations of Flavone Glycosides Profile Underscore the Necessity of Quality Control of Prepared Microctis Folium Slices

“…Moreover, it is stable, is easily obtainable, and can be well separated under the conventional chromatographic conditions. Using vitexin as a reference substance, f x was calculated by the ratio of the peak area and the concentration between the analyte and vitexin (see ( 1 )) [ 23 – 25 ]. Based on the specific f x of objective compound, its concentration ( C x ) in the sample solution could be calculated according to the following formula (see ( 2 )).…”

“…Comparing with ESM, QAMS reduces the expenditure on reference substances and shortens the operation time, especially suitable for quantification of a serial of analogues [ 22 ]. QAMS method has been used for the quality control of some herbal drugs, such as Panax notoginseng [ 23 ], Andrographis herba [ 24 ], and Radix astragali [ 25 ].…”

Comprehensive comparison of the anti‐inflammatory activity and chemical consistency of traditional Chinese medicine formula granules with Ge‐Gen decoction as a representative sample

“…The acute inflammatory model of mice was based on previous reports with some modifications (Huang et al, ; Xiao et al, ). Mice were randomly divided into 12 groups with 10 mice per group: (a) the vehicle control group (mice treated with saline at an equal volume); (b) the model group (mice with inflammation treated with saline at an equal volume); (c) the positive control group [mice with inflammation treated with dexamethasone acetate tablets (3.12 mg/kg, V = 20 mL/kg)] and (d) the nine drug groups [mice with inflammation treated with GGD (S1–S9) at a clinically equivalent quadruple dose (1.48 mg/mL, V = 20 mL/kg) once a day for 3 consecutive days].…”