Quality control tests of two salmonella enrichment media using different inocula

Harvey, R. W. S.; Price, T. H.; Crone, Patricia

doi:10.1017/s0022172400046891

Cited by 21 publications

(17 citation statements)

References 5 publications

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“…(Harvey & Price, 1976). A similar bias fas also been published for S. dublin (Harvey & Price, 1975). It must be emphasized that the current comparison was made without a pre-enrichment stage.…”

Section: Discussionsupporting

confidence: 73%

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Salmonella surveillance with reference to pigs – Cardiff abattoir, 1968–1975

Harvey

Price

Morgan

1977

J. Hyg.

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Section: Discussionsupporting

confidence: 73%

“…It would not allow multiplication of small numbers of salmonellas (Harvey, Price & Crone, 1975). The Cardiff tetrathionate broth, incubated at 43°C would, however, permit growth of minimal numbers of salmonellas as would our seitz-filtered selenite F broth incubated at the same temperature.…”

Section: R W S Harvey T H Price and J Morganmentioning

confidence: 97%

Salmonella surveillance with reference to pigs – Cardiff abattoir, 1968–1975

Harvey

Price

Morgan

1977

J. Hyg.

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“…Once prepared it is ready for use for at least one month. It is also cheap and, unlike some commercial enrichment media (Harvey, Price & Crone, 1975), the different laboratory-prepared lots are uniform in performance, provided malachite green oxalate (Merck) p.a. and MgCl2 .…”

Section: Discussionmentioning

confidence: 99%

Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium

Vassiliadis

Trichopoulos

Kalapothaki

et al. 1981

J. Hyg.

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SUMMARYOne hundred and eighty samples of pork sausages were examined after preenrichment in buffered peptone water (P medium), for the presence of salmonellas. From each pre-enrichment four enrichments were made: (1) 0-1 ml of P medium was inoculated into 10 ml of Rappaport's medium, formula R 10 (R 10/43°C), (2) 1 ml of the P medium was added to 100 ml of R 10 broth (RIO/100 ml/43°C), (3) 1 ml of P medium was inoculated into 10 ml of Muller-Kauffmann tetrathionate broth (MK medium) prepared in accordance with the International Standards Organization document ISO 3565 (MK/43°C) and (4) 10 ml of P medium were added to 100 ml of MK broth (MK/100 ml/43°C). All the enrichments were incubated at 43°C for 48 h. Forty-six and 47 samples were found positive with the first two enrichment methods (R 10/43°C and R 10/100 ml/43°C), while only 16 samples were found positive with the method MK/43°C, and 27 with the method MK/ 100 ml/43 'C. The superiority ofeither one of the two R 10 procedures over either one of the two MK methods is statistically highly significant (paired x2; P < 0-001 in all four comparisons). The superiority of procedure MK/100 ml/43 'C over the method MK/43 'C is also statistically significant (P < 0005). Both R 10/43°C and R 10/100 ml/43°C procedures had a much stronger inhibitory effect on the competing organisms (lactose-and sucrosenegative) than the two MK methods.

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“…Harvey and Price (1975) showed that the performance of different medium brands can vary depending on the inoculation concentration of Salmonella. Inoculation concentration also affects the lag-time of Salmonella Typhimurium (Stephens et al, 1997).…”

Section: Concentration and Condition Of Target Cellsmentioning

confidence: 99%