Quality Control of Reactive Oxygen Species Measurements

Kobayashi, Hiroshi; Guzman, E.G; Mahran, Ali; Sharma, R.; Nelson, Douglas; Agarwal, Ashok

doi:10.1016/s0015-0282(00)01360-1

Cited by 80 publications

(102 citation statements)

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“…The enhanced chemiluminescence assay, however, is the most commonly used method for measuring the TAC in the seminal fluid [12][13][14][15][16][17]. More recently, a reliable, accurate, more rapid and a simple colourimetric method has been developed.…”

Section: Resultsmentioning

confidence: 99%

The Total Antioxidant Power of Semen and Its Correlation with the Fertility Potential of Human Male Subjects

Pahune

Choudhari²,

Muley³

2013

JCDR

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Background: There are growing evidences that the damage which is caused to the spermatozoa by the Reactive Oxygen Species (ROS) plays a key role in the male infertility. The seminal plasma is endowed with many enzymatic and nonenzymatic antioxidants which protect the spermatozoa against oxidative stress.The present study was undertaken by using a simple, colourimetric, ferric reducing, antioxidant power for assessing the total antioxidant power rather than the individual antioxidants. The measurement of the individual antioxidants in the seminal plasma, such as Superoxide Dismutase, Vitamin E, etc. is time consuming, which often requires sophisticated and expensive techniques and these measurements may not correlate with the quality of semen. Aim:To evaluate the total antioxidant capacity of seminal plasma by estimating the Ferric Reducing Antioxidant Power (FRAP) of semen in different groups of subjects and to correlate it with the different seminogram parameters. Material and Methods:The semen samples were obtained from 150 male partners of infertile couples who attended the Reproductive Biology Unit (Infertility Clinic) of the Department of Physiology, MGIMS, Sevagram, who were aged 20-58 years and they were analyzed for the routine seminogram parameters. All the subjects were categorized into two main groups, A. The subjects with abnormal ejaculates, who were further sub classified into the following groups i) Asthenoteratozoospermics (n=25) ii) Oligoasthenoteratozoospermics (n=26) and iii) Azoospermics (n=19) and B. The subjects with normal ejacu lates (n=80). The total antioxidant power was measured spectro photometrically by using the Ferric Reducing Antioxidant Power (FRAP) assay. Results:The Total Antioxidant Capacity (TAC) was found to be significantly lower in the abnormal ejaculates than in the normal ejaculates. A statistically significant positive correlation was observed between the TAC and all the seminogram parameters such as the sperm concentration, sperm motility and sperm morphology (p<0.05). Conclusion:A decreased seminal plasma antioxidant capacity (TAC) could have significant role in the aetiology of impaired sperm functions. So, the TAC may be used as specific biomarker for assessing the oxidative stress in sperms.

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Section: Resultsmentioning

confidence: 99%

The Total Antioxidant Power of Semen and Its Correlation with the Fertility Potential of Human Male Subjects

Pahune

Choudhari²,

Muley³

2013

JCDR

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“…Because OS is an imbalance between levels of ROS production and antioxidant protection in semen, it is conceivable that assessment of OS will rely on the measurement of ROS as well as total antioxidant capacity (TAC) of semen. Levels of ROS can be measured in washed sperm suspensions (after simple wash and resuspension) using chemiluminescence assay (97). Total antioxidant capacity (TAC) in the seminal plasma can be measured using an enhanced chemiluminescence assay (98).…”

Section: Utility Of Seminal Oxidative Stress Test In the Infertility mentioning

confidence: 99%

Role of reactive oxygen species in the pathophysiology of human reproduction

Agarwal

Saleh

Bedaiwy

2003

Fertility and Sterility

1,226

883

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Objective: To summarize the role of reactive oxygen species (ROS) in the pathophysiology of human reproduction. Design: Review of literature. Setting: Fertility research center and obstetrics and gynecology department in a tertiary care facility. Result(s): ROS plays an essential role in the pathogenesis of many reproductive processes. In male-factor infertility, oxidative stress attacks the fluidity of the sperm plasma membrane and the integrity of DNA in the sperm nucleus. Reactive oxygen species induced DNA damage may accelerate the process of germ cell apoptosis, leading to the decline in sperm counts associated with male infertility. ROS mediated female fertility disorders share many pathogenic similarities with the ones on the male side. These similarities include a potential role in the pathophysiology of endometriosis and unexplained infertility. High follicular fluid ROS levels are associated with negative IVF outcomes, particularly in smokers. Moreover, oxidative stress may be responsible in hydrosalpingeal fluid mediated embryotoxicity as well as poor in vitro embryonic development. Conclusion(s):High levels of ROS are detrimental to the fertility potential both in natural and assisted conception states. (Fertil Steril 2003;79:829 -43.

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“…Recent in vitro studies on human sperm and human endothelial cell lines have used 1 hour of in vitro exposure. 53 A decline in ROS levels in semen with time at 37 o C has been demonstrated in a study conducted by Kobayashi et al 54 In a study by Esfandiari et al, ROS levels were significantly higher in semen samples stored at a lower temperature (25 o C vs 37 o C). 55 According to the available guidelines, sensitivity of the experiment should be at the highest level to maximize the possibility of detecting any significant effect(s) of RF-EMW.…”

Section: Dna Integritymentioning

confidence: 84%

Mobile Phone Electromagnetic Waves and Its Effect on Human Ejaculated Semen: An in vitro Study

Veerachari¹,

Vasan²

2012

International Journal of Infertility &Amp; Fetal Medicine

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Mobile phones usage has seen an exponential growth recently. With this increasing demand, the amount of electromagnetic radiation (EMR) exposed is also increasing. Hence, we studied the effect of these radiations on ejaculated human semen and speculate the contribution of these harmful radiations in male infertility. Samples exposed to EMR showed a significant decrease in sperm motility and viability, increase in reactive oxygen species (ROS) and DNA fragmentation index (DFI) compared to unexposed group. We concluded that mobile phones emit electromagnetic waves which lead to oxidative stress in human semen and also cause changes in DNA fragmentation. We extrapolate these findings to speculate that these radiations may negatively affect spermatozoa and impair male fertility.

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Quality Control of Reactive Oxygen Species Measurements

Cited by 80 publications

References 0 publications

The Total Antioxidant Power of Semen and Its Correlation with the Fertility Potential of Human Male Subjects

The Total Antioxidant Power of Semen and Its Correlation with the Fertility Potential of Human Male Subjects

Role of reactive oxygen species in the pathophysiology of human reproduction

Mobile Phone Electromagnetic Waves and Its Effect on Human Ejaculated Semen: An in vitro Study

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