Quality control of dietary supplements: An economic green spectrofluorimetric assay of Raspberry ketone and its application to weight variation testing

Abdelaal, Sarah H.; Azab, Noha F. El; Hassan, Said A.; El-Kosasy, Amira M.

doi:10.1016/j.saa.2021.120032

Cited by 10 publications

(3 citation statements)

References 28 publications

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“…Raspberry ketone (4-(4-hydroxyphenyl)-2-butanone) supplements have also gained popularity among customers due to their ability to burn fat and aid in weight loss. Raspberry ketone is found naturally in raspberry fruit (up to 4.3 mg/kg) [ 14 ] and is used as a flavoring substance [ 15 ]. Processing raspberries extends their shelf life and culinary uses, although the nutritional information among processed forms is limited [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Micronized Powder of Raspberry Pomace as a Source of Bioactive Compounds

Różyło,

Amarowicz,

Janiak

et al. 2023

Molecules

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Red raspberries, which contain a variety of nutrients and phytochemicals that are beneficial for human health, can be utilized as a raw material in the creation of several supplements. This research suggests micronized powder of raspberry pomace production. The molecular characteristics (FTIR), sugar, and biological potential (phenolic compounds and antioxidant activity) of micronized raspberry powders were investigated. FTIR spectroscopy results revealed spectral changes in the ranges with maxima at ~1720, 1635, and 1326, as well as intensity changes in practically the entire spectral range analyzed. The discrepancies clearly indicate that the micronization of the raspberry byproduct samples cleaved the intramolecular hydrogen bonds in the polysaccharides present in the samples, thus increasing the respective content of simple saccharides. In comparison to the control powders, more glucose and fructose were recovered from the micronized samples of the raspberry powders. The study’s micronized powders were found to contain nine different types of phenolic compounds, including rutin, elagic acid derivatives, cyanidin-3-sophoroside, cyanidin-3-(2-glucosylrutinoside), cyanidin-3-rutinoside, pelargonidin-3-rutinoside, and elagic acid derivatives. Significantly higher concentrations of ellagic acid and ellagic acid derivatives and rutin were found in the micronized samples than in the control sample. The antioxidant potential assessed by ABTS and FRAP significantly increased following the micronization procedure.

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Section: Introductionmentioning

confidence: 99%

Micronized Powder of Raspberry Pomace as a Source of Bioactive Compounds

Różyło,

Amarowicz,

Janiak

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

“…Nowadays, protection of the environment and personal health are carefully considered in the field of chemistry, that led to establishing guidelines about how to work according to green principles and increased the number of articles discussing GAC [ 33 , 34 ]. It has been considered in different techniques such as spectrophotometry [ 35 ], spectrofluorimetry [ 36 , 37 ], electrochemistry [ 38 ] and chromatography [ 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

Development of an eco-friendly HPLC method for the stability indicating assay of binary mixture of ibuprofen and phenylephrine

Kelani,

Fayez,

Abdel-Raoof

et al. 2023

BMC Chemistry

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The development and validation of the stability indicating HPLC technique has contributed to the understanding of the stability profile of ibuprofen (IBU) and phenylephrine (PHE). Stability profile was achieved for PHE; the drug was found to be liable to be influenced by stress oxidative conditions; two oxidative degradants (Deg1 & Deg2) were formed and their structures were confirmed using IR and mass spectrometry. The drugs and degradation products were successfully separated using a gradient elution method on YMC-C8 column with 0.1% hexanesulfonic acid and acetonitrile as a mobile phase at pH 6.6. The flow rate was 1.0 mL/min, and a diode array detector operating at 220 nm was used for UV detection. The retention times of degradants Deg1, Deg2, ibuprofen (IBU), and phenylephrine hydrochloride (PHE) were 2.0, 2.2, 3.2 and 7.0 min, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness using ICH guidelines. The linearities of ibuprofen and phenylephrine hydrochloride were in the range of 10–100 μg/mL and 0.3–10 μg/mL, respectively. The % recoveries of the two drugs were found to be 100.75 ± 1.44%, 99.67% ± 1.67, and the LOD was found to be 2.75/mL and 0.09/mL for IBU, and PHE, respectively. The method was successfully applied to the estimation of ibuprofen and phenylephrine hydrochloride combination in pharmaceutical dosage form. The proposed technique was validated using ICH guidelines and its greenness was assessed according to Analytical Eco Scale metric (AES). Molecular docking was used to assess the two drugs and PHE oxidative degradants interaction with the stationary phase and to confirm the outcomes of the proposed method with regard to the order of elution of the two drugs and PHE degradation products. Eco-friendly and environmental safety were assessed through the application of one of the most applicable greenness assessment tool; Analytical Eco Scale metric (AES).

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“…More recently, regulatory bodies have increased their awareness of life-cycle management for analytical methods, so International Council for Harmonisation (ICH) as well as United States Pharmacopeia (USP) Forums discussed the registration of new guidelines that include life-cycle management of analytical methods [25]. The concept of green chemistry is extending to different analytical techniques such as spectrophotometry, spectrofluorimetry, and chromatography [26][27][28][29].…”

mentioning

confidence: 99%

Fluorescence imaging approaches for eco‐friendly determination of perampanel in human plasma and application for therapeutic drug monitoring

et al. 2023

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Antiepileptic drugs are among the most common medications that require therapeutic drug monitoring (TDM). Indeed, TDM provides a realistic approach to adjust drug doses for epilepsy based on plasma concentrations to optimize its clinical outcome.The most common technique for TDM is high-performance liquid chromatography, which has a very low green profile among analytical techniques. Perampanel (PER) is an inherently fluorescent compound that its fluorophore readily allows sensitive and quantitative measurements. This paper describes the development and validation of a sensitive, specific, and eco-friendly spectrofluorimetric method for the determination of PER. Experimental parameters affecting fluorescence intensity of the compound, including solvent dilution, temperature, and excitation wavelength, were studied and optimized. The developed spectrofluorimetric method was established in acetonitrile at λ ex = 295 nm and λ em = 431 nm over a concentration range of 5-60 ng/ml. The adopted method was applied for the determination of PER in human plasma; it was effective in the range of 15-50 ng/ml. The proposed method was found to be sensitive and specific for PER and can be applied successfully in TDM of PER and in quality control laboratories.

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Quality control of dietary supplements: An economic green spectrofluorimetric assay of Raspberry ketone and its application to weight variation testing

Cited by 10 publications

References 28 publications

Micronized Powder of Raspberry Pomace as a Source of Bioactive Compounds

Micronized Powder of Raspberry Pomace as a Source of Bioactive Compounds

Development of an eco-friendly HPLC method for the stability indicating assay of binary mixture of ibuprofen and phenylephrine

Fluorescence imaging approaches for eco‐friendly determination of perampanel in human plasma and application for therapeutic drug monitoring

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