Quality control in the in vitro fertilization laboratory: Comparison of bioassay systems for water quality

Rinehart, John S.; Bavister, Barry D.; Gerrity, Marybeth

doi:10.1007/bf01129569

Cited by 49 publications

(15 citation statements)

References 22 publications

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“…It is well established in animal embryos that a negative correlation exists between the age that embryos are collected from the reproductive tract after fertilisation and their ability to develop to the blastocyst stage in culture (Rinehart et al 1988;Scott et al 1993;Hewitt et al 2003). Furthermore, embryos that are produced after IVF have also been shown to be more sensitive to culture-induced stress.…”

Section: Susceptibility To Stressmentioning

confidence: 96%

Understanding cellular disruptions during early embryo development that perturb viability and fetal development

Lane

Gardner

2005

Reprod. Fertil. Dev.

142

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An inability to regulate ionic and metabolic homeostasis is related to a reduction in the developmental capacity of the embryo. The early embryo soon after fertilisation and up until compaction appears to have a reduced capacity to regulate its homeostasis. The reduced ability to regulate homeostasis, such as intracellular pH and calcium levels, by the precompaction-stage embryo appears to impact on the ability to regulate mitochondrial function and maintain adequate levels of energy production. This reduction in ATP production causes a cascade of events leading to disrupted cellular function and, perhaps ultimately, disrupted epigenetic regulation and aberrant placental and fetal development. In contrast, after compaction the embryo takes on a more somatic cell-like physiology and is better able to regulate its physiology and therefore appears less vulnerable to stress. Therefore, for human IVF it would seem important for the establishment of healthy pregnancies that the embryos are maintained in systems that are designed to minimise homeostatic stress, particularly for the cleavage-stage embryos, as exposure to stress is likely to culminate in impaired embryo function.

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Section: Susceptibility To Stressmentioning

confidence: 96%

Understanding cellular disruptions during early embryo development that perturb viability and fetal development

Lane

Gardner

2005

Reprod. Fertil. Dev.

142

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show abstract

“…The equipment required to make the media, such as glassware, must also be clean and sterile to prevent the transfer of impurities to the media (Gardner and Lane 1999). The water used to make the media should be of the highest quality and be free of bacterial contamination and endotoxins, which themselves can affect embryo development (Rinehart et al 1988). Each new batch of medium should be tested.…”

Section: Culture Mediamentioning

confidence: 99%

To QC or not to QC: the key to a consistent laboratory?

Lane

Mitchell

Cashman

et al. 2008

Reprod. Fertil. Dev.

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A limiting factor in every embryology laboratory is its capacity to grow 'normal' embryos. In human in vitro fertilisation (IVF), there is considerable awareness that the environment of the laboratory itself can alter the quality of the embryos produced and the industry as a whole has moved towards the implementation of auditable quality management systems. Furthermore, in some countries, such as Australia, an established quality management system is mandatory for clinical IVF practice, but such systems are less frequently found in other embryology laboratories. Although the same challenges of supporting consistent and repeatable embryo development are paramount to success in all embryology laboratories, it could be argued that they are more important in a research setting where often the measured outcomes are at an intracellular or molecular level. In the present review, we have outlined the role and importance of quality control and quality assurance systems in any embryo laboratory and have highlighted examples of how simple monitoring can provide consistency and avoid the induction of artefacts, irrespective of the laboratory's purpose, function or species involved.

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“…We have considered hamster embryos to be one of the best models for improving mammalian ART programs, because of their sensitivity to culture environment and in vitro manipulation (1,2). Various metabolisms during preimplantation development have been elucidated using hamster models (3)(4)(5)(6)(7) which significantly contribute to improving human ART programs.…”

mentioning

confidence: 99%

Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro

Gong

Lee

et al. 2007

Fertility and Sterility

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Quality control in the in vitro fertilization laboratory: Comparison of bioassay systems for water quality

Cited by 49 publications

References 22 publications

Understanding cellular disruptions during early embryo development that perturb viability and fetal development

Understanding cellular disruptions during early embryo development that perturb viability and fetal development

To QC or not to QC: the key to a consistent laboratory?

Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro

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