Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro

Oh, Seo Jin; Gong, Seung Pyo; Lee, Seung Tae; Lee, Eun Ju; Lim, Jeong Mook

doi:10.1016/j.fertnstert.2007.01.036

Cited by 87 publications

(57 citation statements)

References 30 publications

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“…The type [2,3,15], intensity [15] and exposure time [27] of light can affect the subsequent development of embryos. In the present study, the light source in the time-lapse system was a red LED light emitting within a narrow wavelength range peaking at 625 nm.…”

Section: Discussionmentioning

confidence: 99%

“…Different measures based on studies of animal model embryos have already been implemented to minimize light exposure of visible [15] or longer wavelength light [2,15,16], and in combination with sensitive camera systems it is possible to obtain high quality images for evaluation without disadvantages on embryonic development and quality [17]. Microscopy typically works with visible light (380-700 nm), where wavelengths <500-550 nm are regarded as harmful to the development and quality of mammalian embryos [2,15]. Increasing exposure time leads to higher total energy dose potentially causing more stress to the embryos.…”

Section: Introductionmentioning

confidence: 99%

“…In mammalian cells, the function of fibroblast cells was disrupted when the total dose of blue light was increased to 10 kJ/m 2 [18]. However, most studies only use light intensity to quantify illumination effect on embryos [3,15,16]. Light at longer wavelength carries lower energy and red light is recommended as a safe illumination source for embryo observation systems [15] and is now routinely applied in timelapse incubation systems [11,12,[19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…However, most studies only use light intensity to quantify illumination effect on embryos [3,15,16]. Light at longer wavelength carries lower energy and red light is recommended as a safe illumination source for embryo observation systems [15] and is now routinely applied in timelapse incubation systems [11,12,[19][20][21]. However, the maximum tolerance of embryos to red light is unknown, and no comparisons have yet been made of an eventual effect of maximizing light exposures to obtain even more morphological details over a full pre-implantation period in-vitro, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Effect of red light on the development and quality of mammalian embryos

Pedersen

Liu

et al. 2014

J Assist Reprod Genet

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Purpose To assess irradiance and total energy dose from different microscopes during the in-vitro embryonic developmental cycle in mouse and pig and to evaluate its effect on embryonic development and quality in pig. Method Spectral scalar irradiance (380-1050 nm) was measured by a fiber-optic microsensor in the focal plane of a dissection microscope, an inverted microscope and a timelapse incubation system. Furthermore, the effect of three different red light levels was tested in the time-lapse system on mouse zygotes for 5 days, and on porcine zona-intact and zona-free parthenogenetically activated (PA) embryos for 6 days. Results The time-lapse system used red light centered at 625 nm and with a lower irradiance level as compared to the white light irradiance levels on the dissection and inverted microscopes, which included more energetic radiation <550 nm. Even after 1000 times higher total energy dose of red light exposure in the time-lapse system, no significant difference was found neither in blastocyst development of mouse zygotes nor in blastocyst rates and total cell number of blastocysts of porcine PA embryos. Conclusions Our results indicate that red light (625 nm, 0.34 W/ m 2 ) used in the time-lapse incubation system does not decrease the development and quality of blastocysts in both mouse zygotes and porcine PA embryos (both zona-intact and zona-free).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of red light on the development and quality of mammalian embryos

Pedersen

Liu

et al. 2014

J Assist Reprod Genet

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“…It has been shown that extensive light exposure may be detrimental to embryo development, and especially that short wavelength light exposure should be minimized [4,27,28,40]. Furthermore, heat due to motion and friction of moving parts; presence of magnetic fields, sheer stress of moving culture dishes and presence of lubricants may each represent theoretical risks to embryo development.…”

Section: Introductionmentioning

confidence: 99%

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Kirkegaard

Hindkjær

Grøndahl

et al. 2012

J Assist Reprod Genet

129

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Purpose Time-lapse monitoring allows for a flexible embryo evaluation and potentially provides new dynamic markers of embryo competence. Before introducing timelapse monitoring in a clinical setting, the safety of the instrument must be properly documented. Accordingly, the aim of this study was to evaluate the safety of a commercially available time-lapse incubator. Methods In a two center, randomized, controlled, clinical trial 676 oocytes from 59 patients in their 2nd or third treatment cycle, age <38 years and ≥8 oocytes retrieved were cultured in the time-lapse incubator or in a conventional incubator. The primary outcome was proportion of 4-cell embryos on day 2. Secondary outcomes were proportion of 7-8 cell embryos on day 3 and proportion of blastocysts on day 5. Implantation pregnancy rates were registered based on presence of fetal heart activity visualized by ultrasound 8 weeks after embryo transfer. Results No significant difference was found between the time-lapse incubator (TLI) and conventional incubator (COI) in proportion of 4-cell embryos on day 2 irrespective of whether data was analyzed according to ITT (RR TLI/COI : 0.81 (0.65; 1.02)) or PP (RR TLI/COI : 0.80 (0.63; 1.01)). Nor were any significant differences detected in the secondary endpoints; i.e. proportion of 7-8-cell embryos on day three ITT (RR TLI/COI : 0.96 (0.73; 1.26)); PP (RR TLI/COI : 0.95 (0.72; 1.26)) and proportion of blastocysts on day five ITT (RR TLI/COI : 1.09 (0.84; 1.41)); PP (RR TLI/COI : 1.09 (0.83: 1.41)). We found no differences in clinical pregnancy rate or implantation rate. Conclusion Culture in the time-lapse incubator supports embryonic development equally to a conventional incubator.

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Effect of blue light‐emitting diode light and antioxidant potential in a somatic cell

Bapary

Takano

Soma

et al. 2019

Cell Biology International

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Light is an indispensable part of routine laboratory work in which conventional light is generally used. Light-emitting diodes (LEDs) have come to replace conventional light, and thus could be a potent target in biomedical studies. Since blue light is a major component of visible light wavelength, in this study, using a somatic cell from the African green monkey kidney, we assessed the possible consequences of the blue spectra of LED light in future animal experiments and proposed a potent mitigation against light-induced damage. COS-7 cells were exposed to blue LED light (450 nm) and the growth and deoxyribonucleic acid (DNA) damage were assessed at different exposure times. A higher suppression in cell growth and viability was observed under a longer period of blue LED light exposure. The number of apoptotic cells increased as the light exposure time was prolonged. Reactive oxygen species (ROS) generation was also elevated in accordance to the extension of light exposure time. A comparison with dark-maintained cells revealed that the upregulation of ROS by blue LED light plays a significant role in causing cellular dysfunction in DNA in a time-dependent manner. In turn, antioxidant treatment has been shown to improve cell growth and viability under blue LED light conditions. This indicates that antioxidants have potential against blue LED light-induced somatic cell damage. It is expected that this study will contribute to the understanding of the basic mechanism of somatic cell death under visible light and maximize the beneficial use of LED light in future animal experiments.

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Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro

Cited by 87 publications

References 30 publications

Effect of red light on the development and quality of mammalian embryos

Effect of red light on the development and quality of mammalian embryos

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Effect of blue light‐emitting diode light and antioxidant potential in a somatic cell

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