This study was designed to assess total petroleum hydrocarbon (THP) concentrations in the surface water and sediment sampled from Woji Creek and to assess potential ecological and human health risk due to petroleum hydrocarbons along the creek. Physicochemical parameters [pH, temperature (T), electrical conductivity (EC), dissolved oxygen (DO), total dissolved solids (TDS)] were in-situ measured from sediment and surface water; hydrological parameters (width, depth and volume) were used to calculate the flow rate (discharge) at different stations of the creek. Trend of TPH in the surface water samples along the creek were as follows: St4 (3.639 AE 1.121 mg/L) > St3 (2.449 AE 0.623 mg/L) > St1 (1.457 AE 0.244 mg/L) > St2 (1.069 AE 0.228 mg/L) > St5 (1.010 AE 0.120 mg/L) Trend of TPH concentration across the creek was as follows: St1 -8.758 AE 0.697 mg/kg > St3 -7.675 AE 0.541 mg/kg > St5 -5.515 AE 0.401 mg/kg > St4 -5.075 AE 0.363 mg/kg > St2 -3.162 AE 0.307 mg/kg. Diagnostic indices indicate that the hydrocarbon in the creek was from petrogenic source. Estimation of ecological risk indicated risk in the surface water but not in the sediment. However, human health risk assessment indicated no risk due to human ingestion of the sediment or surface water.