TIS Biscuit" (cowpea tempe seluang fish) is a high protein and calcium snack which is important to prevent stunting. Stunting is a chronic malnutrition known as short toddlers. Increasing the stunted toddlers can be prevent according to adequate protein and calcium intake. This study aims to determine the levels of essential and nonessential amino acids, calcium, zinc, and phosphorus contained in TIS biscuits. This type of research is true experimental with a completely randomized design with two factors, namely cowpea tempeh flour (85%, 75%, 65%, and 55%) and seluang fish flour (15%, 25%, 35%, 45%). This research includes 3 stages, such as research stage I (organoleptic test of TIS), stage II (test of analytical hierarchy process), and stage III (comparison between control TIS (P0) and the selected biscuit). Statistical analysis of the results of organoleptic and chemical tests using the Independent Sample T-test. The selected biscuit which has the best organoleptic acceptance is TIS P1 (the proportion of 85% cowpea tempeh flour and 15% seluang fish flour). Amino acid, calcium, zinc, and phosphorus levels of TIS increased at P1. Statistical analysis showed significant differences between P0 and P1 on the color, smell, texture, flavour and almost all amino acid levels of TIS. On the other hand, there was no significant difference between P0 and P1 on calcium, zinc, and phosphorus levels. P1 can fulfill 100% of RDA for the lysine, calcium, and zinc by consuming 4 biscuits or 20 g per day as a snack.