Qualitative study of young, adult, and aged wistar rats temporomandibular synovial membrane employing light, scanning, and transmission electron microscopy

Duro, Christiano Cony; Ciena, Adriano Polican; Almeida, Sonia Regina Yokomizo de; Righetti, M.; Grisolia, Débora De Freitas; Issa, João Paulo Mardegan; Silva, Marcelo Cavenaghi Pereira da; Watanabe, Ii-sei

doi:10.1002/jemt.22095

Cited by 6 publications

(5 citation statements)

References 53 publications

(51 reference statements)

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“…The samples were postfixed in a 1% osmium tetroxide aqueous solution for 2 h at 4 °C, dehydrated in increasing alcohol concentrations and dried in a critical point dryer (Balzers CPD‐030) with liquid CO 2 (Duro et al. ; Bolina et al. ).…”

Section: Methodsmentioning

confidence: 99%

Structural and ultrastructural features of the agouti tongue (Dasyprocta aguti Linnaeus, 1766)

et al. 2013

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The agouti (Dasyprocta aguti Linnaeus, 1766) is a wild rodent belonging to the family Dasyproctidae that is found throughout Brazil and feeds on fruits and seeds. The aim of the present study was to describe the following features of the tongue of agouti: its morphological structures, the three-dimensional characteristics of the lingual papillae surface, the connective tissue cores (CTCs) and the epithelial cell ultrastructure. Four types of papillae were observed on the dorsal surface of the tongue with a triangular shape: filiform, fungiform, foliate and vallate. Filiform papillae were distributed throughout the tongue surface, and removal of the epithelial surface revealed conical CTCs and multifilaments. Fungiform papillae were observed in the rostral and middle regions, whereas foliate papillae developed in pairs on the lateral margin of the caudal region. Removal of the epithelium in these regions revealed CTCs with parallel laminar conformation. Vallate papillae were arranged in a V-shape in the caudal region, and their CTCs ranged in shape from elongate to ovoid. The ultrastructural components of the dorsal epithelium were the basal, spinous, granular and keratinised layers. A broad area with cytoplasmic projections was identified in the interface region between the lamina propria and the basal layer. Flattened cells with intermediate filaments were observed in the transitional region between spinous and granular layers. The keratinised layer was composed of superimposed epithelial cells where desmosomes and cell-surface microridges were observed. These structural features, including the three-dimensional aspects of the lingual papillae, the CTCs and the epithelial ultrastructure, indicate that when compared with other animals, particularly other rodent species, the morphological features of the tongue of agouti are relatively well developed, especially regarding foliate and vallate papillae.

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Section: Methodsmentioning

confidence: 99%

Structural and ultrastructural features of the agouti tongue (Dasyprocta aguti Linnaeus, 1766)

et al. 2013

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“…Samples of the ventro‐rostral surface ( n = 4) were immersed in a modified Karnovsky fixative solution, with 2.5% glutaraldehyde and 2% paraformaldehyde in a sodium phosphate buffer solution (0.1 m , pH 7.4), for 48 h at 4°C (Watanabe et al., ; Duro et al., ; Bolina et al., ). The samples were then post‐fixed in a 1% aqueous osmium tetroxide solution for 2 h at 4°C, washed in distilled water, dehydrated in an increasing alcohol series and dried in a Balzers CPD‐020 critical point dryer (Bal‐Tec, Balzers, Liechtenstein) using liquid CO 2 (Ciena et al., ; Reginato et al., ).…”

Section: Methodsmentioning

confidence: 99%

Morphological Aspects of Oral Denticles in the Sharpnose SharkRhizoprionodon lalandii(Müller and Henle, 1839) (Elasmobranchii, Carcharhinidae)

Ciena

Rangel²,

Bruno³

et al. 2015

Anat Histol Embryol

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The oral denticles of some elasmobranchs are found on the surface of the oral cavity and are homologous to those on the body surface, being well developed, independent and non-growing, with varying morphology and distribution depending on the species. The structural and three-dimensional characteristics of oral denticles from the rostro-ventral surface of the sharpnose shark Rhizoprionodon lalandii were described following imaging by both light and scanning electron microscopy. The light microscopy results showed that the triangular shape of the denticles consisted of a base and an apex. Picrosirius staining showed the arrangement of collagen fibres and oral denticles, and a predominance of type-I collagen was found in both structures under polarized light. There was a broad homogeneous distribution of denticles on the ventral surface, forming a leaf-like shape with the cusp facing the caudal region. Interlocking, hexagonal, geometric structures on its rostral side and ridges on the rostral side of the oral denticles were observed under increased magnification. We concluded that the denticle morphology found in R. lalandii differ of others analysed species, and the descriptions of these structures therefore provide important information for the classification of the species. In this species, the main functions can be assigned to help reduce hydrodynamic drag, particularly by this being a species that uses ram ventilation, and to protect the epithelium of the oropharynx of abrasion and parasites.

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“…After this stage, all samples were dehydrated in an increasing series of alcohols and dried in a critical point dryer (Balzers CPD-030) with liquid CO2. Samples were mounted on a metallic base, coated with gold ions (Balzers-040 SDC) [ 22 ], and examined using a scanning electron microscope LEO 435 VP.…”

Section: Methodsmentioning

confidence: 99%

Three-Dimensional Aspects of the Lingual Papillae and Their Connective Tissue Cores in the Tongue of Rats: A Scanning Electron Microscope Study

Reginato

Bolina

Watanabe

et al. 2014

The Scientific World Journal

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The aim of the present study was to describe the tridimensional morphological characteristics of the lingual papillae and their connective tissue cores (CTCs) in Sprague Dawley rats. Four types of papillae were reported on the dorsal surface. Filiform papillae were distributed on the tongue surface and after epithelial maceration a conic and multifilamentary shape of the CTCs was revealed. Fungiform papillae were reported on the rostral and middle regions covered by a squamous epithelium. After the removal of the epithelium, the shape of a volcano with the taste orifice at its top was noted. Foliate papillae were composed of five pairs of epithelial folds situated on the lateral-caudal margin of the tongue. After the removal of the epithelium, they were shown to be limited by thin laminar projections. The vallate papilla with an oval shape was present in the caudal region and delimited by an incomplete groove. The morphological characteristics of the lingual papillae of Sprague Dowley rats, three-dimensional SEM images, and the types of papillae on the dorsal surface were similar to those reported previously in other rodent mammals. The maceration technique revealed the details of extracellular matrix with varied shapes form of connective tissue cores.

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Qualitative study of young, adult, and aged wistar rats temporomandibular synovial membrane employing light, scanning, and transmission electron microscopy

Cited by 6 publications

References 53 publications

Structural and ultrastructural features of the agouti tongue (Dasyprocta aguti Linnaeus, 1766)

Structural and ultrastructural features of the agouti tongue (Dasyprocta aguti Linnaeus, 1766)

Morphological Aspects of Oral Denticles in the Sharpnose SharkRhizoprionodon lalandii(Müller and Henle, 1839) (Elasmobranchii, Carcharhinidae)

Three-Dimensional Aspects of the Lingual Papillae and Their Connective Tissue Cores in the Tongue of Rats: A Scanning Electron Microscope Study

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