Qualitative and Quantitative Sugar Profiling in Olive Fruits, Leaves, and Stems by Gas Chromatography−Tandem Mass Spectrometry (GC-MS/MS) after Ultrasound-Assisted Leaching

Abstract

Qualitative and quantitative profiling of sugars in vegetal materials from Olea europaea cultivars is here reported. Vegetal tissues from olive fruits, leaves, and stems have been characterized by determination of 22 compounds belonging to monosaccharides, disaccharides, trisaccharides, sugar carboxylic acids and alcohols, cyclic polyols, and derived compounds. Sugar isolation was carried out by leaching into a 2:1 dichloromethane/methanol extraction solution under ultrasonic assistance. Multivariate optimization made possible complete isolation of the target fraction in 10 min with an efficiency similar to that provided by a conventional protocol based on 24 h maceration of the vegetal samples. An aliquot of the extract was dried and reconstituted for silylation prior to GC-MS/MS analysis for selective and sensitive identification/quantitation of sugars. Monitoring the target product ions generated after isolation of the precursor ions for each analyte increases the selectivity of the method. The proposed approach is of particular interest for characterization of the sugar fraction in O. europaea, which is of great relevance because of the role of sugars in the metabolism of lipids, proteins, and antioxidants.

Quantitative analysis of diamondoids in crude oils using gas chromatography–triple quadrupole mass spectrometry

“…The fragment ions of the collision are selected by the third quadrupole and then detected. The limits of quantification and detection for triple quadrupole mass spectrometry are as low as several ppb (Frenich et al, 2005;Gomez-Gonzalez et al, 2010). Based on the SRM mode and the optimization of runtime parameters, the triple quadrupole technique can provide maximum selectivity and sensitivity for quantification of diamondoids in crude oils.…”

Indução e histologia de embriões somáticos primários e secundários do híbrido Phalaenopsis Classic Spotted Pink (Orchidaceae)

“…O amido é considerado como o principal carboidrato de reserva nas plantas, atuando como fonte de energia para as alterações metabólicas em plantas ou fornecendo moléculas de açúcares para biossíntese de lipídeos, proteínas, antioxidantes e polissacarídeos (Pescador et al, 2008;Gomez-Gonzalez et al, 2010) e como base de energia para o crescimento, regulação da morfogênese e diferenciação celular das plantas, com grande importância na constituição da estrutura da parede celular (Smeekens, 2000). Análises histoquímicas em jabuticaba-branca (Myrciaria sp.)…”

Identification and assay of carbohydrates in olive drupes by cesium attachment electrospray tandem mass spectrometry (ESI‐MS/MS)

“…[14] In the table, olive processing sugars act as carbon source for microorganisms producing secondary metabolites correlated to the flavor of the product. [13,14] Sugar profiles of olive fruits is poorly described in the literature. Thus, mannitol in olive fruits as well as mannitol and other polyols in many other fruits may be of importance in the metabolic transformation and synthesis of specific first storage material.…”