Qualitative and quantitative phytochemical analysis of Cassia hirsuta seeds

Abstract

Objective: The present study aim was to analyze quantitatively and qualitatively the phytochemicals as well as the investigating the physicochemical properties from seeds of and its chloroform and ethanol extracts. Cassia hirsuta Materials and Methods: In this the physico-chemical evaluation, ash values viz., total ash, acid insoluble ash and water soluble ash, sulphated ash, and extractive values viz., alcohol soluble extractive value, water soluble extractive and chloroform soluble extractive value, and loss on drying were determined. Preliminary phytochemical screening where done for presence of carbohydrates, proteins and amino acids, glycosides, alkaloids, phytosteroids, flavonoids, saponins and tannins and phenolic compounds, and total phenolic content was estimated by Folin Ciocalteu's method and total flavonoid content was measured with the aluminium chloride colorimetric assay. In results it was Results: found that the seed contains various phytochemicals were present in its ethanol and chloroform extracts. The total phenolic content in the ethanol and chloroform extracts were found 13.7±0.4187 and 7.367±0.2987 mg of gallic acid equivalent weight/g of extract respectively and the concentration of flavonoids in plant ethanol and chloroform extracts of were found 114.6±13.33 and 99.56±11.6 mg of quercetin equivalent weight/ g of extract respectively. Cassia hirsuta Conclusion: The present study concludes that the seeds of have the potential to act as a source of useful Cassia hirsuta drugs because of presence of various phytochemical constituents and high concentration of phenolic and flavonoid compounds.