Qualitative and quantitative detection of chicken deoxyribonucleic acid (DNA) in dry dog foods

Holda, K.; Natonek-Wiśniewska, Małgorzata; Krzyscin, P.; Głogowski, Robert

doi:10.1111/jpn.12887

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Similar results to our own research were obtained in previous studies. Hołda et al [47] verified the potential presence of undeclared chicken-derived ingredients in ten commercially available dry dog foods using PCR technology and in most of the analyzed samples the chicken DNA was detectable. However, quantified amounts were mostly low (except one dog food with relatively high amounts of undeclared DNA) and the authors speculated that poor quality poultry fat might been used in the production of tested foods, most likely contaminated with proteins.…”

Section: Discussionmentioning

confidence: 97%

Detection of chicken DNA in commercial dog foods

et al. 2022

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Background These days the number of potential food allergens is very large, but chicken is one of the most common allergens in dogs. Elimination diet is one of the clinical tools for the diagnosis of allergies and allergy tests are not very reliable. The restriction diet is most commonly carried out by feeding pet foods, relying on the ingredients on the label to select an elimination diet not containing previously eaten foods. Unfortunately, mislabeling of pet food is quite common. The purpose of this study was to determine the absence or presence of chicken DNA using both qualitative and quantitative polymerase chain reaction (PCR) analysis methods in dry and wet maintenance complete pet foods for adult dogs. Results were used to verify the declared composition on the labels. Results Eleven out of fifteen (73%) dog foods were produced as declared by the manufacturer, two of which showed the presence of chicken protein as stated on the label. The remaining nine foods contained amounts of chicken DNA below 1%, consistent with declarations that no chicken was added in the composition. Four of tested dog foods (27%) were not produced consistently with the declaration on the packaging. Two dog foods (one dry and one wet) did not contain the claimed chicken protein. In two foods the addition of chicken DNA was detected at the level of over 2% and almost 6%, respectively. Conclusions In this study, we focused on one of the most commonly undeclared animal species on the label—chicken protein—and performed DNA analyzes to investigate possible contamination and mislabeling. The results showed some inaccuracies. However, most of them are trace amounts below 1%, which proves compliance with the label. Our results showed that undeclared animal species can be as common as missing an animal protein declared on the label. The conducted research indicates that both dry and wet analyzed foods should not be recommended as a diagnostic tool in elimination tests, because it may result in false negative results. Over-the-counter maintenance foods for dogs should not be recommended for the diagnosis and treatment of food hypersensitivity.

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Section: Discussionmentioning

confidence: 97%