Application of next-generation sequencing for the identification of herbal products

Lo, Yat Tung; Shaw, Pang Chui

doi:10.1016/j.biotechadv.2019.107450

Cited by 39 publications

(33 citation statements)

References 109 publications

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“…The herbal medicines have been more and more popular to be used worldwide. It has been practiced for centuries that the herbal materials were used for healthcare and therapeutic purposes [46][47][48][49][50][51]. Herbal medicines are not completely harmless.…”

Section: Discussionmentioning

confidence: 99%

“…The quality problems of herbal medicines may lead to ineffective, toxicity [52], cancer [53] or even death [54]. With the increase popularity of using herbal supplements for therapeutic purposes, there are > 920 dietary supplement adulteration cases has found by US Food and Drug Administration (FDA) Tainted Supplements Report from 2007 to 2018 [51,55]. Despite this, herbal medicines have often contributed to the discovery of certain drugs that have advanced human health.…”

Section: Discussionmentioning

confidence: 99%

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The plant original identification of Inner Mongolia Arnebia Radix based on morphology and DNA barcoding

Liu

Zhao

Guo

et al. 2020

Preprint

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Background: It’s a long history for the use of herbal medicines to derive remedies in Chinese, and the popularity of herbal medicines has risen worldwide. It is necessary to assure the safety, efficacy and quality of herbal medicines by the corrected identification. Inner Mongolia Arnebia Radix is an important and historied used herbal medicine in Inner Mongolia.Methods: The methods of morphological characteristics and DNA barcoding was used to identify the plant origin of Inner Mongolia Arnebia Radix.Results: The identification results of morphological characteristics and DNA barcoding showed that the original plant of Inner Mongolia Arnebia Radix was Arnebia szechenyi, which was distinguished with Arnebia guttata, the official original plant of Inner Mongolia Arnebia Radix.Conclusions: We inferred that that the original plant of Inner Mongolia Arnebia Radix was Arnebia szechenyi.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The plant original identification of Inner Mongolia Arnebia Radix based on morphology and DNA barcoding

Liu

Zhao

Guo

et al. 2020

Preprint

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“…Generally, NGS-based detection methods for the authentication of plant foods have been developed, for example for the identification of spices and herbs [56][57][58]. In addition, there are methods for detecting counterfeit honey, which can be used to analyze plant, geographical and insect sources [59,60], and to identify different animal species in meat [61,62], fish [63,64], and dairy products [65].…”

Section: Genomics-based Methodsmentioning

confidence: 99%

Food authentication in real life: How to link nontargeted approaches with routine analytics?

Creydt¹,

Fischer²

2020

Electrophoresis

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In times of increasing globalization and the resulting complexity of trade flows, securing food quality is an increasing challenge. The development of analytical methods for checking the integrity and, thus, the safety of food is one of the central questions for actors from science, politics, and industry. Targeted methods, for the detection of a few selected analytes, still play the most important role in routine analysis. In the past 5 years, nontargeted methods that do not aim at individual analytes but on analyte profiles that are as comprehensive as possible have increasingly come into focus. Instead of investigating individual chemical structures, data patterns are collected, evaluated and, depending on the problem, fed into databases that can be used for further nontargeted approaches. Alternatively, individual markers can be extracted and transferred to targeted methods. Such an approach requires (i) the availability of authentic reference material, (ii) the corresponding highresolution laboratory infrastructure, and (iii) extensive expertise in processing and storing very large amounts of data. Probably due to the requirements mentioned above, only a few methods have really established themselves in routine analysis. This review article focuses on the establishment of nontargeted methods in routine laboratories. Challenges are summarized and possible solutions are presented.

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“…In this study, the ITS2 and rbcL regions were successfully used for the identification of plants in herbal samples at the family-and genus-levels. Fungal contamination was detected in some samples, which could be due to harvesting or storage conditions 25 .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, metabarcoding can be implemented for the quality control and validation of contaminants and species composition in highly processed herbal products 24 . Recent improvements in next-generation sequencing methods further reduced sequencing costs and helped improve the technique for identifying complex multi-ingredient herbal products 25 . Unless regulated, such adulteration may decrease the efficacy of herbal medicines and have adverse effects on consumer health, which will eventually result in economic losses in the herbal market 22 .…”

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confidence: 99%

DNA metabarcoding to unravel plant species composition in selected herbal medicines on the National List of Essential Medicines (NLEM) of Thailand

Urumarudappa¹,

Tungphatthong²,

Prombutara³

et al. 2020

Sci Rep

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Traditional medicines are widely traded across the globe and have received considerable attention in the recent past, with expectations of heightened demand in the future. However, there are increasing global concerns over admixture, which can affect the quality, safety, and efficacy of herbal medicinal products. In this study, we aimed to use DNA metabarcoding to identify 39 Thai herbal products on the Thai National List of Essential Medicines (NLEM) and assess species composition and admixture. Among the products, 24 samples were in-house-prepared formulations, and 15 samples were registered formulations. In our study, DNA metabarcoding analysis using ITS2 and rbcL barcode regions were employed to identify herbal ingredients mentioned in the products. The nuclear region, ITS2, was able to identify herbal ingredients in the products at the genus- and family-levels in 55% and 63% of cases, respectively. The chloroplast gene, rbcL, enabled genus- and family-level identifications in 58% and 73% of cases, respectively. In addition, plant species were detected in larger numbers (Family identified, absolute %) in registered herbal products than in in-house-prepared formulations. The level of fidelity increases concerns about the reliability of the products. This study highlights that DNA metabarcoding is a useful analytical tool when combined with advanced chemical techniques for the identification of plant species in highly processed, multi-ingredient herbal products.

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Application of next-generation sequencing for the identification of herbal products

Cited by 39 publications

References 109 publications

The plant original identification of Inner Mongolia Arnebia Radix based on morphology and DNA barcoding

The plant original identification of Inner Mongolia Arnebia Radix based on morphology and DNA barcoding

Food authentication in real life: How to link nontargeted approaches with routine analytics?

DNA metabarcoding to unravel plant species composition in selected herbal medicines on the National List of Essential Medicines (NLEM) of Thailand

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