Qualitative and quantitative characterization of the total organic matter in a recent marine sediment (Part II)

Klok, J.; Baas, Marianne; Cox, H.C.; Leeuw, J.W. de; Rijpstra, W. Irene C.; Schenck, P.A.

doi:10.1016/0146-6380(84)90048-2

Cited by 38 publications

(28 citation statements)

References 26 publications

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“…Py-GC-MS analysis of fractions E1, E2 and R5 showed that the hot water extract (E1) consisted mainly of (poly)saccharides and some protein (cf., Klok et a!. 1981aKlok et a!. , 1984.…”

Section: Resultsmentioning

confidence: 99%

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Molecular, Radioactive and Stable Carbon Isotope Characterization of Estuarine Particulate Organic Matter

Megens¹,

Plicht²,

Leeuw³

1997

Radiocarbon

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ABSTRACT. Organic matter in sediments and suspended matter is a complex mixture of constituents with different histories, sources and stabilities. To study these components in a suspended matter sample from the Ems-Dollard Estuary, we used combined molecular analysis with pyrolysis/gas chromatography/mass spectrometry and stable and radioactive carbon isotope analyses of the bulk and separated chemical fractions. Carbohydrates and proteins, ca. 50% of the total organic carbon (TOC), are much younger than the bulk sample and have a somewhat higher 613C value. Lipids and the final residue are considerably older and have lower 613C values. The final residue, ca. 17% of the total carbon, consists mainly of aliphatic macromolecules that could be derived from algae or terrestrial plants. The 613C value points to a marine origin.

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“…Py-GC-MS analysis of fractions E1, E2 and R5 showed that the hot water extract (E1) consisted mainly of (poly)saccharides and some protein (cf., Klok et a!. 1981aKlok et a!. , 1984.…”

Section: Resultsmentioning

confidence: 99%

“…The water used was produced by a Millipore milli-Q apparatus. The extraction procedure was based in part on the work of Klok et al (1981aKlok et al ( , 1984. First, samples were extracted by refluxing for 24 h with water.…”

Section: Extraction Proceduresmentioning

confidence: 99%

Molecular, Radioactive and Stable Carbon Isotope Characterization of Estuarine Particulate Organic Matter

Megens¹,

Plicht²,

Leeuw³

1997

Radiocarbon

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“…reported in cyanobacterial mats and in recent marine sediments, where they comprise up to 5-15 wt% of the total neutral monosaccharide yield (Klok et al 1983;1984), and in humic and fulvic acids extracted from soils (Ogner, 1980). Given the wide distribution of these methylsugars in biological and geological materials, they provide little information about the specific source of HMWDOM carbohydrate.…”

Section: -And 3-o-methylrhamnose Along With 2-and 3-o-methylfucose Hmentioning

confidence: 99%

Characterization of methyl sugars, 3-deoxysugars and methyl deoxysugars in marine high molecular weight dissolved organic matter

Panagiotopoulos

Repeta

Johnson

2007

Organic Geochemistry

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“…5.6 (Weckesser et al, 1979;Klok et al, 1984b, and references therein) and in a high molecular-weight material from marine sediments believed to be lipopolysaccharide (Klok et al, 1984a;Klok et al, 1984b). Therefore, they are inferred to be bacterial biomass indicators Moers, 1989;and Klok et al, 1984a;Klok et al, 1984b). The possibility that a considerable portion of the POM pool may consist of bacterial biomass has been explored in algal degradation experiments (Harvey and Macko, 1997); during oxic degradation up to 21.4% of POC could be accounted for by bacterial carbon.…”

Section: 6)mentioning

confidence: 99%

“…However, the separation among aminosugar fragments in the plot of PCl and PC2 (Fig. 5.6 (Weckesser et al, 1979;Klok et al, 1984b, and references therein) and in a high molecular-weight material from marine sediments believed to be lipopolysaccharide (Klok et al, 1984a;Klok et al, 1984b). Therefore, they are inferred to be bacterial biomass indicators Moers, 1989;and Klok et al, 1984a;Klok et al, 1984b).…”

Section: 6)mentioning

confidence: 99%

Compositional heterogeneity within oceanic POM : a study using flow cytometry and mass spectrometry

Minor¹

1998

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This thesis applied direct temperature-resolved mass spectrometry (DT-MS), flow cytometry, and multivariate statistics to the study of marine particulate organic matter (POM) collected from the North Atlantic.DT-MS is an important asset to marine organic geochemistry as a single two minute analysis (with 16 eV, EI* ionization) provides information on polysaccharides, proteins, and lipids within concentrated and desalted samples. Although the molecularlevel information obtained with DT-MS is less detailed than traditional analyses of specific compound classes, DT-MS can act as a useful molecular-level screening technique (as illustrated in this thesis), indicating what samples and compound classes to investigate more thoroughly.In addition to its rapidity, DT-MS only reqires microgram quantities of sample. This sensitivity permits the coupling of DT-MS and preparative flow cytometry. In this thesis, preparative flow cytometry was used to isolate "phytoplankton" and "detritus" (i.e., non-phytoplankton particles) in 2-+53 gm POM. The molecular-level differences between and within small-particle POM (<53 gm), large-particle POM (>53 pm), "phytoplankton" and "detritus" were explored using DT-MS and discriminant analysis. For POM collected from the Mid-Atlantic Bight and from Great Harbor, Woods Hole, MA, small-particle POM contained more phytoplankton chemical characteristics than large-particle POM. In Great Harbor, the molecular-level characteristics of large-particle POM indicated a significant grazer biomass component. On the MAB (in March 1996), the large-particle POM appeared more phytodetrital. "Phytoplankton" was enriched in protein, chlorophyll and lipids as compared to "detritus," which was enriched in selected polysaccharides.As the polysaccharide composition of POM subclasses was a major source of variation, polysaccharides in selected samples were further studied using ammonia and deuterated ammonia CI*DT-MS. Principal component analysis of the resulting NH 3 -CI* spectra indicated that the majority of polysaccharide variation in the selected samples could be explained by a component that appeared related to the degree of degradation of the organic matter.The results from this thesis, coupled with existing work on particulate and dissolved organic matter, were used to support a modified "size-reactivity continuum model" of organic matter cycling. Thesis Supervisor: Timothy EglintonTo my parents, "Uncle Ben," and the old Venture AknowledgementsThis thesis would never have been finished without the help of a great many people. I would like to thank my advisor Tim Eglinton for introducing me to the field, giving me free reign with lots of instruments, and convincing me to do more than I thought I could. Thanks to my committe members are also in order. Without Rob Olson's expertise, the flow cytometer might be in even more pieces today than it was when I started. Jaap Boon's perspective as a mass spectrometrist helped greatly in the interpretation of DT-MS data. Phil Gschwend's inciteful questions ha...

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Qualitative and quantitative characterization of the total organic matter in a recent marine sediment (Part II)

Cited by 38 publications

References 26 publications

Molecular, Radioactive and Stable Carbon Isotope Characterization of Estuarine Particulate Organic Matter

Molecular, Radioactive and Stable Carbon Isotope Characterization of Estuarine Particulate Organic Matter

Characterization of methyl sugars, 3-deoxysugars and methyl deoxysugars in marine high molecular weight dissolved organic matter

Compositional heterogeneity within oceanic POM : a study using flow cytometry and mass spectrometry

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