Characterization of methyl sugars, 3-deoxysugars and methyl deoxysugars in marine high molecular weight dissolved organic matter

Panagiotopoulos, Christos; Repeta, Daniel J.; Johnson, C. G.

doi:10.1016/j.orggeochem.2007.02.005

Cited by 34 publications

(22 citation statements)

References 43 publications

(50 reference statements)

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“…Furthermore, our findings are suggestive of some of the chemical attributes and degradation patterns of naturally occurring DOM. In previous chemical analyses, about 15% of DOM carbohydrate has been shown to consist of methyl sugars (37,38). Our present findings suggest that Alteromonadales (specifically, Idiomarina spp.…”

Section: Discussionmentioning

confidence: 95%

Microbial community transcriptomes reveal microbes and metabolic pathways associated with dissolved organic matter turnover in the sea

McCarren

Becker

Repeta

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Marine dissolved organic matter (DOM) contains as much carbon as the Earth's atmosphere, and represents a critical component of the global carbon cycle. To better define microbial processes and activities associated with marine DOM cycling, we analyzed genomic and transcriptional responses of microbial communities to high-molecularweight DOM (HMWDOM) addition. The cell density in the unamended control remained constant, with very few transcript categories exhibiting significant differences over time. In contrast, the DOM-amended microcosm doubled in cell numbers over 27 h, and a variety of HMWDOM-stimulated transcripts from different taxa were observed at all time points measured relative to the control. Transcripts significantly enriched in the HMWDOM treatment included those associated with two-component sensor systems, phosphate and nitrogen assimilation, chemotaxis, and motility. Transcripts from Idiomarina and Alteromonas spp., the most highly represented taxa at the early time points, included those encoding TonB-associated transporters, nitrogen assimilation genes, fatty acid catabolism genes, and TCA cycle enzymes. At the final time point, Methylophaga rRNA and non-rRNA transcripts dominated the HMWDOM-amended microcosm, and included gene transcripts associated with both assimilatory and dissimilatory single-carbon compound utilization. The data indicated specific resource partitioning of DOM by different bacterial species, which results in a temporal succession of taxa, metabolic pathways, and chemical transformations associated with HMWDOM turnover. These findings suggest that coordinated, cooperative activities of a variety of bacterial "specialists" may be critical in the cycling of marine DOM, emphasizing the importance of microbial community dynamics in the global carbon cycle.icrobial activities drive most of Earth's biogeochemical cycles. Many processes and players involved in these planetary cycles, however, remain largely uncharacterized, due to the inherent complexity of microbial community processes in the environment. Cycling of organic carbon in ocean surface waters is no exception. Though marine dissolved organic matter (DOM) is one of the largest reservoirs of organic carbon on the planet (1), microbial activities that regulate DOM turnover remain poorly resolved (2).Marine DOM is an important substrate for heterotrophic bacterioplankton, which efficiently remineralize as much as 50% of total primary productivity through the microbial loop (3-6). Though some DOM is remineralized on short timescales of minutes to hours, a significant fraction escapes rapid removal. In marine surface waters, this semilabile DOM transiently accumulates to concentrations 2-3 times greater than are found in the deep sea (7), and represents a large inventory of dissolved carbon and nutrients that are potential substrates for marine microbes. Timeseries analyses of semilabile DOM accumulation in temperate and subtropical upper ocean gyres show an annual cycle in DOC inventory with net accumulation following the ...

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Section: Discussionmentioning

confidence: 95%

Microbial community transcriptomes reveal microbes and metabolic pathways associated with dissolved organic matter turnover in the sea

McCarren

Becker

Repeta

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

367

351

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“…In contrast to the low level of hydrolyzable carbohydrates, nuclear magnetic resonance (NMR) analyses indicate an abundance of carbohydrates in at least the high molecular weight fraction of DOM (Benner et al, 1992). These non-hydrolyzable carbohydrates that persist after more labile DOM has been degraded are possibly biosynthesized and belong to the family of acylated polysaccharides (Aluwihare et al, 1997;Panagiotopoulos et al, 2007). The latter observations are of major relevance because they imply that biosynthesized compounds that are presumably not modified by secondary abiotic reactions resist degradation on the long term.…”

Section: The Intrinsic Stability Hypothesismentioning

confidence: 99%

Reasons Behind the Long-Term Stability of Dissolved Organic Matter

Dittmar

2015

Biogeochemistry of Marine Dissolved Organic Matter

131

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“…Hydrolysis of HMWDOM yields an O-methylated sugar fraction that can be purified by chromatography and characterized in detail by 1 H NMR and MS (Panagiotopoulos et al, 2007(Panagiotopoulos et al, , 2013. All seven major neutral sugars recovered by hydrolysis were found to have mono-and di-O-methylated homologues ( Figure 2.5).…”

Section: Figure 23 (A)mentioning

confidence: 99%