Qualitative and quantitative assessment of microbial community in batch anaerobic digestion of secondary sludge

Development and Application of an Enzymatic and Cell Flotation Treatment for the Recovery of Viable Microbial Cells from Environmental Matrices Such as Anaerobic Sludge

“…3). The mean concentrations of bacterial and archaeal 16S gene copies per gram of organic matter converted per millimeter of sludge (1.6 ϫ 10 10 and 1.2 ϫ 10 7 , respectively) were on the same order as values reported in the literature (between 10 9 and 10 11 for Bacteria and between 10 7 and 2 ϫ 10 9 for Archaea) (23,29). The proposed protocol can be further applied to other complex matrices constituted by bioaggregate or biofilm.…”

“…To analyze the structure of the bacterial and archaeal communities, the V3 region of the 16S rRNA gene was amplified. The primers W49F and W104R were used for Bacteria (29), and W274R and W275F were used for Archaea (Table 1). PCR amplifications were carried out using Mastercycler thermocycler (Eppendorf, Hamburg, Germany).…”

Bacterial Consortium and Axenic Cultures Isolated from Activated Sewage Sludge for Biodegradation of Imidazolium-based Ionic Liquid

“…On the other hand, CBM are contiguous amino acids within a carbohydrate-active enzyme with a discreet fold which bears the carbohydrate-binding activity [68]. In contrast to the small number of enzymes devoted to the hydrolysis of the main chain of cellulose, hemicelluloses and pectins (GH 5,6,7,8,9,12,44,45,48 and 74), all three metagenomes displayed a variety of enzymes that digest the side chains of these polymers and oligosaccharides. Families of GH 2 and 3, which contain a large range of glycosidases cleaving nonreducing carbohydrates in oligosaccharides and the side chains of hemicelluloses and pectins, were particularly abundant in the bioreactor samples (29.10% of all GH-UP30, 27.56%; UP40, 29.55%; P30, 24.20%).…”

“…Sludge disposal by landfill or incineration may no longer be feasible in the near future due to land scarcity, high waste charge and increasingly stringent environmental control regulations [4,5]. As a result, the strategy for sludge management is shifting towards its reutilization as a potential source for renewable energy [6][7][8]. In this regard, the anaerobic digestion process represents an attractive means of sludge reduction while producing renewable energy in the form of biogas [8,9].…”

Performance of and methanogenic communities involved in an innovative anaerobic process for the treatment of food wastewater in a pilot plant

“…The ratio of ARC and BAC was 4.6 ± 0.2%, which is similar to that reported from other anaerobic processes treating organic waste. 16,17) The copy numbers for MBT, MMB, and MSL were found to be 4.4 × 10 7 , 1.6 × 10 8 , 4.9 × 10 6 copies/mL, respectively. As a result of numerical conversion of these results to methanogenic fraction numbers, the factions of MMB, MBT, and MSL were calculated to cover 62, 17, and 2.0% of all methanogens, respectively, showing that MMB was predominant in this system.…”