Performance of and methanogenic communities involved in an innovative anaerobic process for the treatment of food wastewater in a pilot plant

Lee, Seungyong; Lee, Bowon; Han, Gyu-Seong; Kim, Woong

doi:10.1080/09168451.2015.1018122

Cited by 1 publication

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References 36 publications

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“…Hydrogenotrophic methanogens were also found in extreme conditions, such as acidic peat [ 9 ]. Therefore, the advantages of hydrogenotrophic biomethanation, including biogas upgrading [ 10 ], its high CO 2 → CH 4 conversion ratio [ 11 ], and its tolerance to environmental perturbation in the field [ 12 ], can be used in anaerobic digestion under various conditions, such as in acidogenic reactors [ 9 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Microbial Community in an Acidic Hollow-Fiber Membrane Biofilm Reactor (Hf-MBfR) Used for the Biological Conversion of Carbon Dioxide to Methane

Shin

Jeon

et al. 2015

PLoS ONE

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Hydrogenotrophic methanogens can use gaseous substrates, such as H2 and CO2, in CH4 production. H2 gas is used to reduce CO2. We have successfully operated a hollow-fiber membrane biofilm reactor (Hf-MBfR) for stable and continuous CH4 production from CO2 and H2. CO2 and H2 were diffused into the culture medium through the membrane without bubble formation in the Hf-MBfR, which was operated at pH 4.5–5.5 over 70 days. Focusing on the presence of hydrogenotrophic methanogens, we analyzed the structure of the microbial community in the reactor. Denaturing gradient gel electrophoresis (DGGE) was conducted with bacterial and archaeal 16S rDNA primers. Real-time qPCR was used to track changes in the community composition of methanogens over the course of operation. Finally, the microbial community and its diversity at the time of maximum CH4 production were analyzed by pyrosequencing methods. Genus Methanobacterium, related to hydrogenotrophic methanogens, dominated the microbial community, but acetate consumption by bacteria, such as unclassified Clostridium sp., restricted the development of acetoclastic methanogens in the acidic CH4 production process. The results show that acidic operation of a CH4 production reactor without any pH adjustment inhibited acetogenic growth and enriched the hydrogenotrophic methanogens, decreasing the growth of acetoclastic methanogens.

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