Qualitative and Quantitative Analysis of the Phenolic Constituents from<i>Orthosiphon aristatus</i>

Sumaryono, Wahono; Proksch, Peter; Wray, Victor; Witte, Ludger; Hartmann, Thomas

doi:10.1055/s-2006-960060

Cited by 97 publications

(66 citation statements)

References 5 publications

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“…stamineus contains several chemically active constituents; one of the most important classes of compounds is the phenolic group. Some researchers have isolated many phenolic compounds including lipophilic flavones, flavonol glycosides, and caffeic acid derivatives, such as 2,3-dicaffeoyltartaric acid and rosmarinic acid, which have been identified and quantified by High Performance Liquid Chromatography (Sumaryono et al, 1991). O. stamineus leaves contain polymethoxylated flavones, sinensetin, tetramethylscutellarein and 30-hydroxy-5,6,7,40-tetramethoxyflavone, as reported by Pietta et al (1998).…”

Section: Introductionmentioning

confidence: 90%

Interaction Study: The Effect of Orthosiphon Stamineus Extract on Human Cytochrome P450

Purwantiningsih

Hussin

2014

Indonesian J. Pharm.

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Herbal remedies are often used concomitantly with prescribed medication, which leads to an increase in the potential of herb-drug interactions. Orthosiphon stamineus is one of the popular herbal preparations that is traditionally used, especially as a diuretic or for the elimination of kidney stones. The inhibitory effect of O. stamineus extract on human cytochrome P450 isoforms, namely CYP1A2, CYP3A4, CYP2C9 and CYP2D6, has been determined using luminescent methods. The final concentrations of O. stamineus extract tested were 0.01, 0.1, 1, 10, 100 and 1000µg/mL. IC 50 value was used to assess the modulation potencies for each CYP isoform. The extract showed a moderate inhibition towards CYP2C9 with the IC 50 of 20.12µg/mL and showed IC 50 of 49.90, 89.24 and 97.82µg/mL for CYP1A2, CYP2D6 and CYP3A4, respectively. These data suggest that O. stamineus extract may potentiate the herb-drug interaction via CYP inhibition.

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Section: Introductionmentioning

confidence: 90%

Interaction Study: The Effect of Orthosiphon Stamineus Extract on Human Cytochrome P450

Purwantiningsih

Hussin

2014

Indonesian J. Pharm.

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“…Usually separation methods using both, column [3][4][5] and planar [6][7][8][9] chromatography are preferred. Generally thin layer analyses are carried out on silica plates.…”

Section: Figure 1 Structure Of Rosmarinic Acid (I) Sinesetin (Ii) Amentioning

confidence: 99%

Characterization of Orthosiphon Stamineus Benth extracts by reversed-phase thin layer Chromatographic methods

Oláh¹,

Hanganu²,

Bodoki³

et al. 2017

Studia UBB Chemia

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ABSTRACT. TLC is a powerful method used for separation of complex mixtures such as plant extracts. Employing different TLC techniques the separations can be improved. This paper presents a study of the Orthosiphon stamineus Benth extracts using isocratic reversed-phase thin layer chromatography (RP-TLC) and reversed-phase automated multiple development technique (RP-AMD). Methanol (SI) and a mixture of methanolwater-methyl acetate (SII) were used as extraction agents. Orthosiphon stamineus Benth. belongs to the Lamiaceae family. Its leaves contain rosmarinic acid, sinesetine and eupatorine as main compounds. After TLC separation the bioactive compounds from plant extracts were identified by comparison of the R f values and in situ UV-Vis spectra with those of the standards and quantified using the calibration method. The rosmarinic acid was better extracted in the solvent mixture methanol-water-methyl acetate (10:10:80, v/v), while the sinesetine and eupatorine, which are more lipophilic, were better extracted in methanol. The study revealed the AMD technique superiority in comparison with the isocratic one.

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“…These were extracted from the callus tissue with hot water or methanol (Sumaryono et al, 1991), then assayed quantitatively at A 765 with the FolinCiocalteau reagent (Singleton and Rossi, 1965), and expressed as mg of caffeic acid per kg fresh weight (FW).…”

Section: Extraction and Quantification Of Total Phenolsmentioning

confidence: 99%

Induction of Phenolic Compounds in Wheat (Triticum aestivum L.) Tissue Cultures by Streptomycin

Hassan

2007

Zeitschrift Für Naturforschung C

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The tissue cultures of wheat (Triticum aestivum L.) were induced from the mature embryos (explants) of the dry grains and grown on MS medium containing kinetin (0.1 mg/l) and 2,4 D (1.0 mg/l). The cultures were incubated for two weeks at (25 ð 2) ∞C under a light/dark regime (16 h light daily). The formed calli were subcultured at the beginning of the stationary growth phase (15 days) with fresh MS medium containing 0, 5, 10, 25, 50, 100, 150 mg/l streptomycin elicitor and maintained for two weeks for three subcultures. A significant increase in phenylalanine ammonia lyase (PAL) activity coincided with the increase of the total phenolic compounds after elicitation with streptomycin. Maximum induction was recorded during the first two weeks, then gradually declined during the rest of the experimental period, but the values attained were still markedly higher than that of the control. The endogenous cinnamic acid content was also increased significantly with the increase in PAL activity making about 2Ð18% of the total phenolic acids. The growth and accumulation of phenolic compounds were inversely related. However, accumulation of phenolic compounds became limited for growth of wheat tissue culture especially during the long term cultivation.

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Qualitative and Quantitative Analysis of the Phenolic Constituents fromOrthosiphon aristatus

Cited by 97 publications

References 5 publications

Interaction Study: The Effect of Orthosiphon Stamineus Extract on Human Cytochrome P450

Interaction Study: The Effect of Orthosiphon Stamineus Extract on Human Cytochrome P450

Characterization of Orthosiphon Stamineus Benth extracts by reversed-phase thin layer Chromatographic methods

Induction of Phenolic Compounds in Wheat (Triticum aestivum L.) Tissue Cultures by Streptomycin

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