Qualitative and Quantitative Analysis of the Major Constituents in Shexiang Tongxin Dropping Pill by HPLC-Q-TOF-MS/MS and UPLC-QqQ-MS/MS

Abstract

Shexiang Tongxin dropping pill (STP) is a traditional Chinese medicine formula that consists of total saponins of ginseng, synthetic Calculus bovis, bear gall, Venenum bufonis, borneol and Salvia miltiorrhiza. STP has been widely used in China and Southeast Asia for the treatment of cardiovascular diseases. In this study, a qualitative analytical method using high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was developed for identification of the major constituents in STP. Based on the retention time and MS spectra, 41 components were identified by comparison with reference compounds and literature data. Moreover, using ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry in multiple-reaction monitoring mode, we quantified 13 of the identified constituents (ginsenoside Rg1, ginsenoside Rk3, cinobufagin, arenobufagin, bufalin, resibufogenin, tanshinone IIA, taurine, tauroursodeoxycholic acid, taurocholic acid, cholic acid, deoxycholic acid, and chenodeoxycholic acid). These results suggest that this new approach is applicable for the OPEN ACCESSMolecules 2015, 20 18598 routine analysis and quality control of STP products and provides fundamental data for further in vivo pharmacokinetical studies.

Effect of Shexiang Tongxin dropping pill on stable coronary artery disease patients with normal fractional flow reserve and coronary microvascular disease

“…STDP is a formulate traditional Chinese Medicine widely used to treating clinical cardiovascular disease, especially in stable angina pectoris in China and South Asia. [ 13 – 16 ] It is a complex herb formula, ultra performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC QqQ-MS/MS) Quantitative Analysis revealed 13 major chemical constituents in STDP, [ 17 ] which has the functions of dilating blood vessels, anti-inflammatory and reducing endothelial damage. Previous studies have revealed that STDP has a significant improvement on myocardial ischemia.…”

Simultaneous determination of ten constituents in chaiqin qingning capsule by high-performance liquid chromatography coupled with triple-quadrupole mass spectrometry

“…Fourth, the values of LOD and LOQ under our method were less than the compounds in other references. [ 5 6 ]…”

Qualitative and quantitative determination of YiXinShu Tablet using ultra high performance liquid chromatography with Q Exactive hybrid quadrupole orbitrap high-resolution accurate mass spectrometry

“…Overall, a total of 105 compounds were identified or tentatively characterized from YXS Tablet. These compounds include 25 phenanthraquinones, 11 lactones, 19 lignans, 24 acids, and 26 other compounds [13][14][15][16][17][18][19][20][21][22]. Their retention times, molecular formulas, high-resolution MS data, and high-resolution MS 2 fragment ions are summarized in Table 1.…”