Qualitative and quantitative analyses of nucleosides and nucleobases in Ganoderma spp. by HPLC–DAD-MS

Characterisation of nucleosides and nucleobases in Mactra veneriformis by high performance liquid chromatography coupled with diode array detector-mass spectrometry (HPLC–DAD–MS)

“…Several methods, including HPLC-ELSD (Wang et al, 2009), HPLC-DAD (Yang & Li, 2008), HPLC-MS (Gao et al, 2007;Guo et al, 2010;Yamaoka et al, 2010), and CE-MS (Yang, Ge, Yong, Tan, & Li, 2009) were used to determine the nucleosides and nucleobases derived from dietary food or TCM, such as Cordyceps, Ganoderma spp., and Ziziphus jujuba. However, there is still no proper method for nucleosides and nucleobases analysis in M. veneriformis.…”

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“…In addition to being precursors in nucleic acid synthesis, nucleotides were reported to enhance immune response 29 , influence metabolism of fatty acids contributes to iron absorption in gut and improve gastrointestinal tract repair after damage. Furthermore, nucleosides and nucleobases in G. lucidum were reported to be capable of inhibiting platelet aggregation and lowering the elevated serum aldolase level of experimental model mice 30 . It had been suggested that the increased dietary nucleoside-nucleotide mixture might be associated with decreases in the ageinduced deterioration of brain morphology and certain memory tasks 31 .…”

Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography–mass spectrometry

“…It is worthy noting that most of the injection samples were always extracted by water or low concentration methanol aqueous solutions without any clear up step. However, in our preliminary experiment, HPLC system pressure was raised by the assaying samples prepared by the described method [23], and the peaks were distorted when multiple analyses were undertaken. This phenomenon was probably due to the accumulation of dissolved sample protein in the column head, which were co-extracted by high polarity solvent.…”

“…Due to the remarkable activities of nucleosides and nucleobases, and their research blank in Tuber genus, it was necessary to qualitatively and quantitatively compare the formulation of nucleosides and nucleobases between Tuber fruiting-bodies and fermentation mycelia. The current techniques for assaying nucleosides and nucleobases mainly include liquid chromatography (LC) [22,23] or capillary electrophoresis (CE) [24,25] combined with ultraviolet detector (UV) or mass spectra (MS). It is worthy noting that most of the injection samples were always extracted by water or low concentration methanol aqueous solutions without any clear up step.…”