Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells

Bon, Pierre; Maucort, Guillaume; Wattellier, Benoît; Monneret, Serge

doi:10.1364/oe.17.013080

Cited by 421 publications

(348 citation statements)

References 18 publications

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“…Talbot interferometers and their variations, such as multilateral shearing interferometers or modified Hartmann mask interferometers, are widely used at visible [12][13][14][15] and infrared wavelengths [16]. A Talbot interferometer at hard x-rays can use a π-phase shift checkerboard pattern with pitch p as the imaging object.…”

Section: Introductionmentioning

confidence: 99%

High-accuracy wavefront sensing for x-ray free electron lasers

Liu

Seaberg

Zhu

et al. 2018

Optica

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Systematic understanding and real-time feedback capability for x-ray free electron laser (FEL) accelerator and optical components are critical for scientific experiments and instrument performance. Single-shot wavefront sensing enables characterization of the intensity and local electric field distribution at the sample plane, something that is important for understanding scientific experiments such as nonlinear studies. It can also provide feedback for alignment and tuning of the FEL beam and instrumentation optics, leading to optimal instrument performance and greater operational efficiency. A robust, sensitive, and accurate single-shot wavefront sensor for x-ray FEL beams using single grating Talbot interferometry has been developed. Experiments performed at the Linac Coherent Light Source (LCLS) demonstrate 3σ sensitivity and accuracy, both better than λ∕100, and retrieval of hard x-ray (λ 0.13 nm, E 9.5 keV) wavefronts in 3D. Exhibiting high performance from both unfocused and focused beams, the same setup can be used to systematically study the wavefront from the FEL output, beam transport optics, and endstation focusing optics. This technique can be extended for use with softer and harder x rays with modified grating configurations.

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Section: Introductionmentioning

confidence: 99%

High-accuracy wavefront sensing for x-ray free electron lasers

Liu

Seaberg

Zhu

et al. 2018

Optica

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“…One approach in this direction consists in quantitative phase microscopy (QPM), for which contrast relies on refractive index values. Several approaches were developed, involving either coherent, 5,6 partially coherent [7][8][9] or incoherent [10][11][12] light. QPM is typically a full-¯eld imaging technique which does not involve optical sectioning, although recent advances led to three-dimensional (3D) imaging with these approaches.…”

Section: Modern Linear Methodsmentioning

confidence: 99%

Multimodal label-free microscopy

Pavillon

Fujita

Smith

2014

J. Innov. Opt. Health Sci.

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This paper reviews the di®erent multimodal applications based on a large extent of label-free imaging modalities, ranging from linear to nonlinear optics, while also including spectroscopic measurements. We put speci¯c emphasis on multimodal measurements going across the usual boundaries between imaging modalities, whereas most multimodal platforms combine techniques based on similar light interactions or similar hardware implementations. In this review, we limit the scope to focus on applications for biology such as live cells or tissues, since by their nature of being alive or fragile, we are often not free to take liberties with the image acquisition times and are forced to gather the maximum amount of information possible at one time. For such samples, imaging by a given label-free method usually presents a challenge in obtaining su±cient optical signal or is limited in terms of the types of observable targets. Multimodal imaging is then particularly attractive for these samples in order to maximize the amount of measured information. While multimodal imaging is always useful in the sense of acquiring additional information from additional modes, at times it is possible to attain information that could not be discovered using any single mode alone, which is the essence of the progress that is possible using a multimodal approach.

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“…Recent developments based on lateral shearing interferometry do not require such interventions, but instead act on the detector. 41 Whilst such quantitative phase imaging methods expand applications to real-time follow-up of cellular dry mass and intracellular dynamics of specific organelles such as lysosomes, 41 transmission microscopy remains limited when it comes to monitoring subcellular processes, despite its excellent sensitivity and speed. The major reasons for that are the high transparency of typical biological samples (e.g., cells) and the lack of specific dyes for identifying specific proteins or chromatin structures.…”

Section: A Two-dimensional Light Microscopymentioning

confidence: 99%

Invited Review Article: Advanced light microscopy for biological space research

Vos

Beghuin²,

Schwarz

et al. 2014

Review of Scientific Instruments

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As commercial space flights have become feasible and long-term extraterrestrial missions are planned, it is imperative that the impact of space travel and the space environment on human physiology be thoroughly characterized. Scrutinizing the effects of potentially detrimental factors such as ionizing radiation and microgravity at the cellular and tissue level demands adequate visualization technology. Advanced light microscopy (ALM) is the leading tool for non-destructive structural and functional investigation of static as well as dynamic biological systems. In recent years, technological developments and advances in photochemistry and genetic engineering have boosted all aspects of resolution, readout and throughput, rendering ALM ideally suited for biological space research. While various microscopy-based studies have addressed cellular response to space-related environmental stressors, biological endpoints have typically been determined only after the mission, leaving an experimental gap that is prone to bias results. An on-board, real-time microscopical monitoring device can bridge this gap. Breadboards and even fully operational microscope setups have been conceived, but they need to be rendered more compact and versatile. Most importantly, they must allow addressing the impact of gravity, or the lack thereof, on physiologically relevant biological systems in space and in ground-based simulations. In order to delineate the essential functionalities for such a system, we have reviewed the pending questions in space science, the relevant biological model systems, and the state-of-the art in ALM. Based on a rigorous trade-off, in which we recognize the relevance of multi-cellular systems and the cellular microenvironment, we propose a compact, but flexible concept for space-related cell biological research that is based on light sheet microscopy.

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Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells

Cited by 421 publications

References 18 publications

High-accuracy wavefront sensing for x-ray free electron lasers

High-accuracy wavefront sensing for x-ray free electron lasers

Multimodal label-free microscopy

Invited Review Article: Advanced light microscopy for biological space research

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