QTLs DETECTION UNDER NATURAL INFECTION OF Moniliophtora perniciosa IN A CACAO F2 PROGENY WITH SCAVINA-6 DESCENDANTS

Santos, Francisca Feitosa Jucá; Lopes, Uilson Vanderlei; Pires, José Luís; Melo, Gilson Roberto Pires; Gramacho, Karina Peres; Clément, Didier

doi:10.21757/0103-3816.2014v26n1p65-72

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…This work demonstrates that clovamide content of 'Sca6' is likely a major factor in the observed difference in leaf resistance compared to 'ICS1. ' 'Sca6' has also been heavily relied upon as a source of resistance to Witches' Broom Disease (WBD, caused by Moniliophthora perniciosa; Pires et al, 2012;Feitosa Jucá Santos et al, 2014). Although the effect of clovamide against M. perniciosa was not evaluated, the potential for this compound's importance in WBD resistance exists.…”

Section: Discussionmentioning

confidence: 99%

Clovamide, a Hydroxycinnamic Acid Amide, Is a Resistance Factor Against Phytophthora spp. in Theobroma cacao

Knollenberg

Lambert

et al. 2020

Front. Plant Sci.

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The hydroxycinnamic acid amides (HCAAs) are a diverse group of plant-specialized phenylpropanoid metabolites distributed widely in the plant kingdom and are known to be involved in tolerance to abiotic and biotic stress. The HCAA clovamide is reported in a small number of distantly related species. To explore the contribution of specialized metabolites to disease resistance in cacao (Theobroma cacao L., chocolate tree), we performed untargeted metabolomics using liquid chromatography – tandem mass spectrometry (LC-MS/MS) and compared the basal metabolite profiles in leaves of two cacao genotypes with contrasting levels of susceptibility to Phytophthora spp. Leaves of the tolerant genotype ‘Scavina 6’ (‘Sca6’) were found to accumulate dramatically higher levels of clovamide and several other HCAAs compared to the susceptible ‘Imperial College Selection 1’ (‘ICS1’). Clovamide was the most abundant metabolite in ‘Sca6’ leaf extracts based on MS signal, and was up to 58-fold higher in ‘Sca6’ than in ‘ICS1’. In vitro assays demonstrated that clovamide inhibits growth of three pathogens of cacao in the genus Phytophthora, is a substrate for cacao polyphenol oxidase, and is a contributor to enzymatic browning. Furthermore, clovamide inhibited proteinase and pectinase in vitro, activities associated with defense in plant-pathogen interactions. Fruit epidermal peels from both genotypes contained substantial amounts of clovamide, but two sulfated HCAAs were present at high abundance exclusively in ‘Sca6’ suggesting a potential functional role of these compounds. The potential to breed cacao with increased HCAAs for improved agricultural performance is discussed.

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Section: Discussionmentioning

confidence: 99%

Clovamide, a Hydroxycinnamic Acid Amide, Is a Resistance Factor Against Phytophthora spp. in Theobroma cacao

Knollenberg

Lambert

et al. 2020

Front. Plant Sci.

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“…In the present work we used five M. perniciosa genomes; representative of previously described subpopulations within the Solanaceae (2) and of the Malvaceae (3) families [ 9 , 20 – 22 ], and one M. roreri genome obtained from Theobroma cacao at the Instituto de Cultivos Tropicales (ICT), Peru.…”

Section: Methodsmentioning

confidence: 99%

“…Each isolate is specific of subpopulation: Mp4145 (CEPLAC/CEPEC, Bahia, Brazil accession number 4145) and Mp1441 (CEPLAC/CEPEC, Bahia, accession number 1441) isolated from a susceptible cacao genotype collected in 2003 and 2012, respectively, and represents two separate incursions of M. perniciosa in Bahia [ 20 ]. Mp178 (CEPLAC/CEPEC, Bahia, accession number 4413) and Mp4071 (CEPLAC/CEPEC, Bahia, accession number 4071) were derived from the wild solanaceous hosts lobeira in Minas Gerais and Caiçara in Bahia (both from Brazil); that do not infect cacao [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations

et al. 2018

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BackgroundThe hemibiotrophic pathogens Moniliophthora perniciosa (witches’ broom disease) and Moniliophthora roreri (frosty pod rot disease) are among the most important pathogens of cacao. Moniliophthora perniciosa has a broad host range and infects a variety of meristematic tissues in cacao plants, whereas M. roreri infects only pods of Theobroma and Herrania genera. Comparative pathogenomics of these fungi is essential to understand Moniliophthora infection strategies, therefore the detection and in silico functional characterization of effector candidates are important steps to gain insight on their pathogenicity.ResultsCandidate secreted effector proteins repertoire were predicted using the genomes of five representative isolates of M. perniciosa subpopulations (three from cacao and two from solanaceous hosts), and one representative isolate of M. roreri from Peru. Many putative effectors candidates were identified in M. perniciosa: 157 and 134 in cacao isolates from Bahia, Brazil; 109 in cacao isolate from Ecuador, 92 and 80 in wild solanaceous isolates from Minas Gerais (Lobeira) and Bahia (Caiçara), Brazil; respectively. Moniliophthora roreri showed the highest number of effector candidates, a total of 243. A set of eight core effectors were shared among all Moniliophthora isolates, while others were shared either between the wild solanaceous isolates or among cacao isolates. Mostly, candidate effectors of M. perniciosa were shared among the isolates, whereas in M. roreri nearly 50% were exclusive to the specie. In addition, a large number of cell wall-degrading enzymes characteristic of hemibiotrophic fungi were found. From these, we highlighted the proteins involved in cell wall modification, an enzymatic arsenal that allows the plant pathogens to inhabit environments with oxidative stress, which promotes degradation of plant compounds and facilitates infection.ConclusionsThe present work reports six genomes and provides a database of the putative effectorome of Moniliophthora, a first step towards the understanding of the functional basis of fungal pathogenicity.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4875-7) contains supplementary material, which is available to authorized users.

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A reference high-density genetic map of Theobroma grandiflorum (Willd. ex Spreng) and QTL detection for resistance to witches’ broom disease (Moniliophthora perniciosa)

Mournet

Albuquerque

Alves

et al. 2020

Tree Genetics & Genomes

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QTLs DETECTION UNDER NATURAL INFECTION OF Moniliophtora perniciosa IN A CACAO F2 PROGENY WITH SCAVINA-6 DESCENDANTS

Cited by 6 publications

References 8 publications

Clovamide, a Hydroxycinnamic Acid Amide, Is a Resistance Factor Against Phytophthora spp. in Theobroma cacao

Clovamide, a Hydroxycinnamic Acid Amide, Is a Resistance Factor Against Phytophthora spp. in Theobroma cacao

Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations

A reference high-density genetic map of Theobroma grandiflorum (Willd. ex Spreng) and QTL detection for resistance to witches’ broom disease (Moniliophthora perniciosa)

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