QTL mapping in Vigna radiata × Vigna umbellata population uncovers major genomic regions associated with bruchid resistance

Mariyammal, Irulappan; Seram, Devina; Samyuktha, Santhi Madhavan; Karthikeyan, Adhimoolam; Dhasarathan, M.; Jayakodi, Murukarthick; Kennedy, J. S.; Malarvizhi, D.; Yang, Tae-Jin; Pandiyan, M.

doi:10.1007/s11032-019-1006-3

Cited by 14 publications

(7 citation statements)

References 57 publications

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“…The results of the current study are concurrent with a recent report on the identification of QTL controlling bruchid resistance in V. radiata [ 94 ]. It was found that a major QTL containing various candidate genes such as R-gene families and TFs are responsible for bruchid defense in black gram.…”

Section: Discussionsupporting

confidence: 88%

Bruchid beetle ovipositioning mediated defense responses in black gram pods

et al. 2021

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Background Black gram [Vigna mungo (L)] seeds are a rich source of digestible protein and dietary fibre, both for human and animal consumption. However, the quality and quantity of the Vigna seeds are severely affected by bruchid beetles during storage. Therefore, analyses of the expression of the bruchid induced transcript dynamics in black gram pods would be helpful to understand the underlying defense mechanism against bruchid oviposition. Results We used the RNAseq approach to survey the changes in transcript profile in the developing seeds of a moderately resistant cultivar IC-8219 against bruchid oviposition using a susceptible cultivar T-9 as a control. A total of 96,084,600 and 99,532,488 clean reads were generated from eight (4 each) samples of IC-8219 and T-9 cultivar, respectively. Based on the BLASTX search against the NR database, 32,584 CDSs were generated of which 31,817 CDSs were significantly similar to Vigna radiata, a close relative of Vigna mungo. The IC-8219 cultivar had 630 significantly differentially expressed genes (DEGs) of which 304 and 326 genes up and down-regulated, respectively. However, in the T-9 cultivar, only 168 DEGs were identified of which 142 and 26 genes up and down-regulated, respectively. The expression analyses of 10 DEGs by qPCR confirmed the accuracy of the RNA-Seq data. Gene Ontology and KEGG pathway analyses helped us to better understand the role of these DEGs in oviposition mediated defense response of black gram. In both the cultivars, the most significant transcriptomic changes in response to the oviposition were related to the induction of defense response genes, transcription factors, secondary metabolites, enzyme inhibitors, and signal transduction pathways. It appears that the bruchid ovipositioning mediated defense response in black gram is induced by SA signaling pathways and defense genes such as defensin, genes for secondary metabolites, and enzyme inhibitors could be potential candidates for resistance to bruchids. Conclusion We generated a transcript profile of immature black gram pods upon bruchid ovipositioning by de novo assembly and studied the underlying defense mechanism of a moderately resistant cultivar.

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Section: Discussionsupporting

confidence: 88%

Bruchid beetle ovipositioning mediated defense responses in black gram pods

et al. 2021

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“…Percentage of (%) seed damage: Number of seeds damaged/Number of seeds taken × 100. Based on seed damage percent, the genotypes were categorized as highly resistant (0-10%), resistant (10.1-20%), moderately resistant (20.1-40%), susceptible (40.1-80%), and highly susceptible (80.1-100%) [10].…”

Section: Assay Methodology For Screening Mungbean Genotypes and Resis...mentioning

confidence: 99%

“…Among the insect pests, bruchids (Callosobruchus spp. Coleoptera, Bruchidae) are major insects causing severe damage to seeds in storage conditions [8][9][10]. The two most common species infecting mungbean seeds are Callosobruchus chinensis (L.) and Callosobruchus maculatus (F.) [11].…”

Section: Introductionmentioning

confidence: 99%

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The Hunt for Mungbean (Vigna radiata (L.) Wilczek) Genotypes and Breeding Lines Resistance to South Indian Bruchid Strain

et al. 2022

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Mungbean (Vigna radiata) is an important short-season legume widely cultivated in Asia, particularly India. It is highly susceptible to bruchids and developing bruchid resistance is an important goal in mungbean breeding programs. In the present study, 52 mungbean genotypes were evaluated for bruchid resistance based on the “no-choice test” and identified two highly resistant genotypes (V2802BG and V2709) with no adult emergence and seed damage. Further, these two resistant genotypes were crossed with six high-yielding bruchid susceptible cultivars (CO 5, CO 6, CO 7, CO 8, VBN 2, and VBN 3), and 12 independent F1 populations were generated. Of these, one population derived from CO 6 × V2802BG was selected (based on the good combining ability of the parents) and forwarded to later generations to trace the bruchid-resistant lines. A total of 159 F2:3 families were screened for bruchid resistance, and the results showed that seven families were highly resistant, whereas the remainder were resistant to highly susceptible. Further, those seven families were evaluated in F4 and F5 generations. As a result, five highly resistant lines (BSR-GG-1-49-3-1, BSR-GG-1-56-2-2, BSR-GG-1-160-5-3, BSR-GG-1-170-2-4, and BSR-GG-1-198-1-4) with good agronomic performances were identified. The newly developed lines could be tested in multi-location trials and then be utilized as a potential source of genetic material for improving the bruchid resistance in mungbean breeding programs.

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“…With this prospect in mind, an attempt was made to differentiate noticeable characters between the potentially misidenti ed species commonly found in Southern India (especially Tamil Nadu), C. maculatus (Vir and Jindal 1981) and C. chinensis (Haines 1981; Khandwe et al 1997) with an aim to clear the doubts and di culties faced during species identi cation by those researchers (including the rst author during the initial research period) working on bruchid related research, except taxonomic studies, such as management, host plant resistance evaluation, inheritance of seed resistance studies, identi cation of molecular markers linked with the resistance, QTL mapping of the resistance gene in different pulses, etc. (Young et al 1993 Mariyammal et al 2019). Comparisons between certain striking morphological characters of these two bruchid species which will be useful in better and easy identi cation are made in regard to some of the original descriptions and subsequent published literatures, including those of the other rare Callosobruchus species found in India viz.…”

Section: Introductionmentioning

confidence: 99%

Differentiating Callosobruchus of South India with Special Reference to Callosobruchus maculatus - A Useful Guide for Entomologists and Non-Entomologists

Seram

Natesan

Muthaiyan³

et al. 2022

Preprint

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Losses of seed produce in pulses due to seed beetles is a very serious problem faced by farmers and traders during storage. Resistance (or susceptibility) and the degree of damage in legume seeds differ with respect to different bruchid species. The seed beetles, Callosobruchus chinensis and C. maculatus, are the two most widespread bruchid species in Southern India, which is being potentially misidentified. With this perspective, fundamental studies on their morphology are of great importance for correct identification, species specific resistant variety improvement and management. For a reliable and effective resistance evaluation in breeding program, a known, identified species or biotype must be employed. In this study, dissection of striking external morphological characters of both the species was done for comparisons. Type of antennae and hind femur are two common characters used for distinguishing these allied species. However, the most reliable genus and species indicators are variation in the spines on the underside of hind femur. The possible distinguishing characters of these two widespread species are illustrated in light of previously published literatures including other rare Callosobruchus species found in India viz. C. nigritus and C. orientalis, reported from Manipur, India. Furthermore, Scanning Electron Microscope study of South Indian C. maculatus strain revealed the presence of different types of sensilla on mouthparts and antennal regions, which had different functions. It is expected that this paper will provide practical information to both entomologists and non-entomologists working on bruchids especially plant breeders involved in resistant varietal development in the tropics and sub-tropical countries.

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QTL mapping in Vigna radiata × Vigna umbellata population uncovers major genomic regions associated with bruchid resistance

Cited by 14 publications

References 57 publications

Bruchid beetle ovipositioning mediated defense responses in black gram pods

Bruchid beetle ovipositioning mediated defense responses in black gram pods

The Hunt for Mungbean (Vigna radiata (L.) Wilczek) Genotypes and Breeding Lines Resistance to South Indian Bruchid Strain

Differentiating Callosobruchus of South India with Special Reference to Callosobruchus maculatus - A Useful Guide for Entomologists and Non-Entomologists

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