QTL mapping for the number of branches and pods using wild chromosome segment substitution lines in soybean [Glycine max (L.) Merr.]

Abstract

Annual wild soybean characterized with more number of branches and pods may contain favourable exotic genes/alleles for improving the yield potential of cultivated soybeans. To evaluate the wild alleles/segments, the chromosome segment substitution line population SojaCSSLP3 comprising 158 lines with N24852 (wild) as the donor and NN1138-2 (cultivated) as the recurrent parent was tested under three environments. The phenotypic data along with 198 simple sequence repeat markers were analysed for qualitative trait loci (QTL)/segments associated with the number of branches on the main stem (BN) and number of pods per plant (PN) using the inclusive composite interval mapping procedure (RSTEP-LRT-ADD model) of ICIM version 3.0. The analysis was carried out for individual environments due to a significant G £ E interaction. A total of eight QTL/segments associated with BN and eight QTL/segments associated with PN were detected under the three environments, with all the wild segments having positive effects. Among these, two QTL/segments for each of the two traits could be detected under two or three environments and three QTL/segments could be detected for both traits. Four QTL/segments associated with BN and one QTL/segment associated with PN were identified only in SojaCSSLP3, not reported for cultivated crosses in the literature. The detected wild segments may provide materials for further characterization, cloning and pyramiding of the alleles conferring the two traits. Keywords: chromosome segment substitution lines; number of branches on the main stem; number of pods per plant; QTL/segment detection; wild soybean (Glycine soja Sieb. et Zucc.) * Corresponding authors.

QTL Location and Epistatic Effect Analysis of 100-Seed Weight Using Wild Soybean (Glycine soja Sieb. & Zucc.) Chromosome Segment Substitution Lines

“…Satt504 was shown to be related to seed weight in an analysis of an F 2:3 population of 186 families derived from a cross between Pak Chong 2 and Laos 7122 [ 38 ]. A QTL for the protein filling rate was found to be close to Satt504 [ 39 ]. Similarly, a QTL for seed shape was found to be close to Satt582, based on analyses of three densely mapped recombinant inbred populations, each with 192 segregants (Minsoy × Archer, Minsoy × Noir1, and Noir1 × Archer) [ 40 ].…”

Genetic Characterization of the Soybean Nested Association Mapping Population

“…Single‐family‐based analysis of genotypes together with phenotypes is the most commonly used method to map the QTL associated with these traits. In soybean, >180 phenotypic traits, such as seed yield (Chung et al, 2003; Palomeque et al, 2009; Wang et al, 2004, 2014), yield components (He et al, 2014; Jeong et al, 2012; Kato et al, 2014), morphological traits (Lee et al, 2014, Yamanaka et al, 2001), seed composition (Brummer et al, 1997; Warrington et al, 2015), resistance to diseases (Pham et al, 2013; Wu et al, 2009) and pests (Rector et al, 1998, Terry et al, 2000, Zhang et al, 2009), and abiotic stresses (Abdel‐Haleem et al, 2012; Lee et al, 2004), have been analyzed with this approach as documented in SoyBase (http://www.soybase.org/search/qtllist_by_symbol.php). Linkage mapping does not require high marker density because of the limited number of recombination events (REs) that occur during selfing and the limited number of lines typically used in such studies.…”

Current Perspectives on Introgression Breeding in Food Legumes

“…In this study, four QTLs related with plant height, as well as node numbers per plant, have been identified ( Wang et al, 2013 ). The same CSSL population was used for mining and fine mapping of QTLs underlying seed quality traits including size and shape, as well as other agronomic traits ( Wang et al, 2012 , 2013 ; He et al, 2014 ).…”