QTL detection of production traits for the Kuruma prawn Penaeus japonicus (Bate) using AFLP markers

Growth is a priority trait from the point of view of genetic improvement. Molecular markers linked to quantitative trait loci (QTL) have been regarded as useful for marker-assisted selection (MAS) in complex traits as growth. Using an intermediate F2 cross of slow and fast growth parents, a genetic linkage map of Pacific whiteleg shrimp, Litopenaeusvannamei , based on amplified fragment length polymorphisms (AFLP) and simple sequence repeats (SSR) markers was constructed. Meanwhile, QTL analysis was performed for growth-related traits. The linkage map consisted of 451 marker loci (429 AFLPs and 22 SSRs) which formed 49 linkage groups with an average marker space of 7.6 cM; they spanned a total length of 3627.6 cM, covering 79.50% of estimated genome size. 14 QTLs were identified for growth-related traits, including three QTLs for body weight (BW), total length (TL) and partial carapace length (PCL), two QTLs for body length (BL), one QTL for first abdominal segment depth (FASD), third abdominal segment depth (TASD) and first abdominal segment width (FASW), which explained 2.62 to 61.42% of phenotypic variation. Moreover, comparison of linkage maps between L . vannamei and Penaeus japonicus was applied, providing a new insight into the genetic base of QTL affecting the growth-related traits. The new results will be useful for conducting MAS breeding schemes in L . vannamei .

show abstract

“…monodon [27], P . japonicus [8] and L . vannamei [9], but is almost similar to those generated 7.1 in P .…”

Section: Discussionmentioning

confidence: 99%

“…To date, genetic mapping of penaeid species has been slow and concentrated mostly on a few important cultured species such as Penaeus monodon [7], P . japonicus [8], L . vannamei [9], and P .…”

Section: Introductionmentioning

confidence: 99%

Genetic Map Construction and Quantitative Trait Locus (QTL) Detection of Growth-Related Traits in Litopenaeus vannamei for Selective Breeding Applications

2013

View full text Add to dashboard Cite

show abstract

“…The amplification does not require any prior genomic sequence information. In decapod species such as Fenneropenaeus chinensis (Wang et al, 2012), Penaeus japonicas (Li et al, 2006a), Litopenaeus vannamei (Andriantahina et al, 2013) and Portunus trituberculatus , previous linkage maps were constructed mainly relying on AFLP or RAPD markers. However, AFLP and RAPD markers are unknown DNA sequences and should be further converted into reliable sequence characterized amplified region (SCAR) markers when used in QTL location and positional cloning of target genes.…”

mentioning

confidence: 99%

A first generation microsatellite-based linkage map of the Chinese mitten crab Eriocheir sinensis and its application in quantitative trait loci (QTL) detection

et al. 2016

View full text Add to dashboard Cite

“…Based on the above observations, it was suggested that there would be a relative concentrated region that controlled part of growth traits between 72.603 cM and 73.603 cM po sition on maternal LG1, the linked marker covered in this region was SSR27 with position 72.640 cM on maternal LG1, which separated only by 0.037 cM to QTL q standardl 01 and was one of the co dominant markers containing the richest genetic information. Through IM (interval mapping) and CIM (compound interval mapping) methods, in another similar study, Li et al observed three QTLs associated with body length and weight, carapace length, sharing the same position on paternal LG1 of P. japonicus, along with the smaller variance explained value of carapace than that of body length [40]. Together with this study re sults, it might be concluded that growth related genes concentrated at a certain genome region in shrimps and were all multi effect loci.…”

Section: Qtl Mapping Of Body Length and Other Economic Traits Of F Cmentioning

confidence: 94%

Integration genetic linkage map construction and several potential QTLs mapping of Chinese shrimp (Fenneropenaeus chinensis) based on three types of molecular markers

Wang

Kong

et al. 2012

Russ J Genet

View full text Add to dashboard Cite

In this study, totally 54 selected polymorphic SSR loci of Chinese shrimp (Fenneropenaeus chin ensis), in addition with the previous linkage map of AFLP and RAPD markers, were used in consolidated linkage maps that composed of SSR, AFLP and RAPD markers of female and male construction, respec tively. The female linkage map contained 236 segregating markers, which were linked in 44 linkage groups, and the genome coverage was 63.98%. The male linkage map contained 255 segregating markers, which were linked in 50 linkage groups, covering 63.40% of F. chinensis genome. There were nine economically impor tant traits and phenotype characters of F. chinensis were involved in QTL mapping using multiple QTL map ping strategy. Five potential QTLs associated with standard length (q standardl 01), with cephalothorax length (q cephal 01), with cephaloghorax width (q cephaw 01), with the first segment length (q firsel 01) and with anti WSSV (q antiWSSV 01) were detected on female LG1 and male LG44 respectively with LOD > 2.5. The QTL q firsel 01 was at 73.603 cM of female LG1. Q antiWSSV 01 was at 0 cM of male LG44. The variance explained of these five QTLs was from 19.7-33.5% and additive value was from -15.9175 to 7.3675. The closest markers to these QTL were all SSR, which suggested SSR marker was superior to AFLP and RAPD in the QTL mapping.

show abstract

QTL detection of production traits for the Kuruma prawn Penaeus japonicus (Bate) using AFLP markers

Cited by 40 publications

References 36 publications

Genetic Map Construction and Quantitative Trait Locus (QTL) Detection of Growth-Related Traits in Litopenaeus vannamei for Selective Breeding Applications

Genetic Map Construction and Quantitative Trait Locus (QTL) Detection of Growth-Related Traits in Litopenaeus vannamei for Selective Breeding Applications

A first generation microsatellite-based linkage map of the Chinese mitten crab Eriocheir sinensis and its application in quantitative trait loci (QTL) detection

Integration genetic linkage map construction and several potential QTLs mapping of Chinese shrimp (Fenneropenaeus chinensis) based on three types of molecular markers

Contact Info

Product

Resources

About