QTL Analysis of Low Temperature Induced Browning in Soybean Seed Coats

Abstract

Exposure of soybean [Glycine max (L.) Merr.] to chilling temperatures at flowering stage induces browning around the hilum of the seed coats. The brown pigmentation spoils the external appearance of soybean seeds and reduces their commercial value. Our previous studies revealed that pigmentation was controlled by a few major genes, and one of the genes is closely associated with a maturity gene. This study was conducted to further investigate inheritance of pigmentation using DNA markers. Fifty-eight F(2) plants derived from a cross between a tolerant cv. Koganejiro and a sensitive cv. Kitakomachi were exposed to 15 degrees C for 2 weeks beginning 8 days after anthesis. Genotypes of 522 genetic markers were determined using the F(2) plants. Composite interval mapping revealed 5 quantitative trait loci (QTLs) for pigmentation, pig1 to pig5 (pig1 in molecular linkage group A2 [MLG A2], pig2 in MLG B1, pig3 in MLG C2, pig4 in molecular linkage group (MLG), and pig5 in MLG N) and 4 QTLs for flowering date, fd1 to fd4 (fd1 in MLG C1, fd2 in MLG C2, fd3 in MLG J, and fd4 in MLG L). Based on the relative location with markers, fd2 and fd4 probably correspond to E1 and E3, respectively. pig3 and fd2 were found at a similar position, and logarithm of odds (LOD) score plots for pigmentation and flowering date almost overlapped around this region. Considering the fact that pig3 had the most intense effects on pigmentation, E1 is presumed to be the maturity gene that profoundly affects pigmentation. Further, E3 has a small effect on pigmentation in accordance with the previous reports. These results support the idea that soybean maturity genes control low temperature-induced pigmentation with various intensities specific to each maturity gene. QTLs for seed coat pigmentation with small or no impact on maturity identified in this study may be useful in breeding for chilling tolerance.

Tracking footprints of selection associated with soybean adaptation to Central-East Europe environments

“…The QTLs associated with seed characteristics were also found in the proximity of marker Satt234 (Salas et al 2006). Several QTLs affecting yield, days to flowering and maturity were mapped to the region of Satt557 on the chromosome 6 (Specht et al 2001;Githiri et al 2007;Guzman et al 2007). This locus also bears a major QTL for the photoperiod insensitivity with Satt557, the closest marker to the …”

“…The identification of genes/ QTLs underlying phenotypic variation in soybean (Glycine max (L.) Merr.) has been based mostly on biparental linkage mapping (Salas et al 2006;Githiri et al 2007;Guzman et al 2007) and association mapping (Hu et al 2014;Wen et al 2014) approaches. To make association between phenotypic differences and the variability at molecular level more precise, these methods require a high level of segregating genetic and phenotypic variation at the genes of Electronic supplementary material The online version of this article (doi:10.1007/s10681-014-1313-z) contains supplementary material, which is available to authorized users.…”

Translational Genomics in Agriculture: Some Examples in Grain Legumes

“…Resistance to biotic stresses Angular leaf spot QTL 22-64 Oblessuc et al, 2012;Teixeira et al, 2005 (Khedikar et al, 2010;Liang et al, 2009a;Qin et al, 2012;Sujay et al, 2012;Wang et al, 2013 (Gautami et al, 2012b;Ravi et al, 2011;Varshney et al, 2009b) Gautami et al, 2012a;Liang et al, 2009;Ravi et al, 2011;Selvaraj et al, 2009;Shirasawa et al, 2012;Varshney et al, 2009b) (Continued on next page) (Liang et al, 2009;Pandey et al, 2012c;Sarvamangala et al, 2011;Selvaraj et al, 2009;Shirasawa et al, 2012) Pea Plant height QTL 19-65 (Tullu et al, 2008;Tar'an et al, 2003b;Dirlewanger et al, 1994) (Atibalentja et al, 2005;Guo et al, 2006;Guo et al, 2008;Han et al, 2008;Huynh et al, 2010;Kazi et al, 2008;Ruben et al, 2006;Shi et al, 2008;Silva and Danielle, 2008;Vuong et al, 2008;Wang et al, 2010;Winter et al, 2007) (Continued on next page) Funatsuki et al, 2005;Githiri et al, 2006;Githiri et al, 2007;…”

Recent Advances in Physiological and Genetic Studies on Chilling Tolerance in Soybean

“…An association of maturity loci with chilling tolerance has been demonstrated for seed quality as well as for seed yield 1,7,30 . It is common with chilling tolerance for seed yield that the E1 and e4 alleles promoted tolerance for browning in seed coats, while the e3 allele had a negative effect 30 in contrast to its positive effect on chilling tolerance for seed yield.…”