QTL Analysis of Fusarium Root Rot Resistance in an Andean × Middle American Common Bean RIL Population

Wang, Weijia; Jacobs, James J.; Chilvers, Martin I.; Mukankusi, Clare Mugisha; Kelly, James D.; Cichy, Karen A.

doi:10.2135/cropsci2017.10.0608

Cited by 18 publications

(22 citation statements)

References 32 publications

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“…In a previous study, FRR was determined to be controlled by root genotype and root vigor played an important part in resistance (Cichy et al, 2007). Wang et al (2018) demonstrated that resistant lines had a slightly higher root biomass and hypothesized that some of the QTL associated with FRR resistance are more likely related to root biomass. High resistance consistently associated with root architecture, which indicates that these may be dependent traits and need to be considered when selecting lines for resistance breeding (Strock et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Sources of Resistance to Fusarium solani and Associated Genomic Regions in Common Bean Diversity Panels

et al. 2020

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Common bean (Phaseolus vulgaris L.) production worldwide is hampered by Fusarium root rot (FRR), which is caused by Fusarium solani. Screening for FRR resistance on a large scale is notoriously difficult and often yields inconsistent results due to variability within the environment and pathogen biology. A greenhouse screening assay was developed incorporating multiple isolates of F. solani to improve assay reproducibility. The Andean (ADP; n = 270) and Middle American (MDP; n = 280) Diversity Panels were screened in the greenhouse to identify genetic factors associated with FRR resistance. Forty-seven MDP and 34 ADP lines from multiple market classes were identified as resistant to FRR. Greenhouse phenotyping repeatability was confirmed via five control lines. Genome-wide association mapping using ∼200k SNPs was performed on standard phenotyping score 1-9, as well as binary and polynomial transformation of score data. Sixteen and seven significant genomic regions were identified for ADP and MDP, respectively, using all three classes of phenotypic data. Most candidate genes were associated with plant immune/defense mechanisms. For the ADP population, ortholog of glucan synthase-like enzyme, senescence-associated genes, and NAC domain protein, associated with peak genomic region Pv08:0.04-0.18 Mbp, were the most significant candidate genes. For the MDP population, the peak SNPs Pv07:15.29 Mbp and Pv01:51 Mbp mapped within gene models associated with ethylene response factor 1 and MAC/Perforin domain-containing gene respectively. The research provides a basis for bean improvement through the use of resistant genotypes and genomic regions for more durable root rot resistance.

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Section: Discussionmentioning

confidence: 99%

Sources of Resistance to Fusarium solani and Associated Genomic Regions in Common Bean Diversity Panels

et al. 2020

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“…In a second bean study, one QTL was identified for FRR explaining just 10% of the variance (LOD 3.2) [ 47 ]. Conversely, in a recent bean study, QTLs for F. solani resistance identified 17 QTL related to FRR explaining at most 16% of the variance (LOD 5.84) [ 48 ]. In the closely related legume, lentil, Fusarium root rot was identified as a problem by Hwang et al [ 49 ] and noted as an increasing production constraint [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

Confirmation of Fusarium root rot resistance QTL Fsp-Ps 2.1 of pea under controlled conditions

Coyne

Porter²,

Boutet

et al. 2019

BMC Plant Biol

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BackgroundDry pea production has increased substantially in North America over the last few decades. With this expansion, significant yield losses have been attributed to an escalation in Fusarium root rots in pea fields. Among the most significant rot rotting pathogenic fungal species, Fusarium solani fsp. pisi (Fsp) is one of the main causal agents of root rot of pea. High levels of partial resistance to Fsp has been identified in plant genetic resources. Genetic resistance offers one of the best solutions to control this root rotting fungus. A recombinant inbred population segregating for high levels of partial resistance, previously single nucleotide polymorphism (SNP) genotyped using genotyping-by-sequencing, was phenotyped for disease reaction in replicated and repeated greenhouse trials. Composite interval mapping was deployed to identify resistance-associated quantitative trait loci (QTL).ResultsThree QTL were identified using three disease reaction criteria: root disease severity, ratios of diseased vs. healthy shoot heights and dry plant weights under controlled conditions using pure cultures of Fusarium solani fsp. pisi. One QTL Fsp-Ps 2.1 explains 44.4–53.4% of the variance with a narrow confidence interval of 1.2 cM. The second and third QTL Fsp-Ps3.2 and Fsp-Ps3.3 are closely linked and explain only 3.6–4.6% of the variance. All of the alleles are contributed by the resistant parent PI 180693.ConclusionWith the confirmation of Fsp-Ps 2.1 now in two RIL populations, SNPs associated with this region make a good target for marker-assisted selection in pea breeding programs to obtain high levels of partial resistance to Fusarium root rot caused by Fusarium solani fsp. pisi.Electronic supplementary materialThe online version of this article (10.1186/s12870-019-1699-9) contains supplementary material, which is available to authorized users.

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“…Recent studies have mapped several traits associated with FRR on chromosomes Pv02, Pv03, Pv05, Pv07 and Pv11 using SSR and SNP markers (Hagerty et al, 2015; Kamfwa et al, 2013; Nakedde et al, 2016; Wang et al, 2018). In another study, Soltani et al (2018) reported high susceptibility of Andean genotypes to Pythium spp.…”

Section: Discussionmentioning

confidence: 99%

“…The second region was 162.39 Kb flanked by markers SNP ss715645960 (44.95 Mb) and ss715645959 (44.79 Mb) in LD ( r 2 > .61; D ′ > 0.72). Wang et al (2018) reported QTL for FRR resistance and control root dry weight on Pv02 in the CAL96 × MLB‐49‐89A RIL population. The QTL FRR2.2 CM at 35.40 Mb and FRR2.3 CM at 30.57 Mb for FRR disease severity in the greenhouse and field colocalized with RTWC2.1 CM at 32.62 Mb for control root dry weight and STWC2.1 CM at 46.31 Mb for control shoot dry weight, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The QTL FRR2.2 CM at 35.40 Mb and FRR2.3 CM at 30.57 Mb for FRR disease severity in the greenhouse and field colocalized with RTWC2.1 CM at 32.62 Mb for control root dry weight and STWC2.1 CM at 46.31 Mb for control shoot dry weight, respectively. In addition, Soltani et al (2018) detected an association signal on Pv02 for root weight under non‐flooded condition at 32.00 Mb and survival score at 47.30 Mb under flooded conditions near QTL RTWC2.1 CM and STWC2.1 CM for control root dry weight and control shoot dry weight, respectively (Wang et al, 2018). Conversely, the genomic region for PRR tagged by SNP S02_25507837 (25.50 Mb) is >5 Mb upstream of peak marker linked to FRR2.3 CM at 30.57 Mb and STWC2.1 CM at 46.31 Mb is >2 Mb from marker SNP ss715645960 (44.95 Mb).…”

Section: Discussionmentioning

confidence: 99%

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Genome‐wide association analysis of resistance to Pythium ultimum in common bean (Phaseolus vulgaris)

et al. 2020

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Common bean is the most important edible grain legume and dietary component in the world (Gepts et al., 2008). Bean contributes significantly to food and nutritional security of millions in sub-Saharan Africa and the developing world, providing a cheap protein source and micronutrients such as iron and zinc (Broughton et al., 2003; Graham et al., 2007), and income for smallholder farmers. However, productivity of common bean in many parts of Central and South America, and Africa is constrained by several diseases that includes root rots (RR) (Abawi and Corrales, 1990). Common bean RR caused by a complex of numerous soil-borne pathogenic fungi including Fusarium solani f. sp. phaseoli, Pythium spp., Rhizoctonia solani and Sclerotium rolfsii Sacc. are the primary cause of crop failures among subsistence farmers in the East African Region (Abawi and Corrales,

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QTL Analysis of Fusarium Root Rot Resistance in an Andean × Middle American Common Bean RIL Population

Cited by 18 publications

References 32 publications

Sources of Resistance to Fusarium solani and Associated Genomic Regions in Common Bean Diversity Panels

Sources of Resistance to Fusarium solani and Associated Genomic Regions in Common Bean Diversity Panels

Confirmation of Fusarium root rot resistance QTL Fsp-Ps 2.1 of pea under controlled conditions

Genome‐wide association analysis of resistance to Pythium ultimum in common bean (Phaseolus vulgaris)

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