Confirmation of Fusarium root rot resistance QTL Fsp-Ps 2.1 of pea under controlled conditions

Coyne, Clarice J.; Porter, Lyndon D.; Boutet, Gilles; Ma, Yue; McGee, Rebecca J.; Lesné, Angélique; Baranger, Alain; Pilet‐Nayel, Marie‐Laure

doi:10.1186/s12870-019-1699-9

Cited by 30 publications

(49 citation statements)

References 44 publications

(81 reference statements)

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“… Coyne et al (2019) reported a significant QTL ( Fsp-Ps2.1 ) that accounts for 44.4 to 53.4% of the phenotypic variance for resistance to Fsp and this QTL shows a confidence interval of 1.2 cM. Fsp-Ps2.1 was mapped within the interval of the pigmented flower/anthocyanin pigmentation gene called as gene A in that study.…”

Section: Discussionmentioning

confidence: 86%

“…This defensin was also found to colocalize with a major QTL (mpIII-4) involved in resistance to Mycosphaerella pinodes in pea (Prioul-Gervais et al, 2007). DRR230 gene does not co-localize with any of the major QTLs identified by Coyne et al (2019).…”

Section: Pathogenesis-related (Pr) Genesmentioning

confidence: 89%

“…Fsp-Ps 2.1, the major QTL found to be associated with Fsp tolerance in pea (Coyne et al, 2019), was annotated using the transcriptome data generated in this study to determine if there are any differentially expressed genes located in the selected genomic region. Fsp-Ps 2.1 explains 44.4-53.4% of the phenotypic variance, and it is located on chromosome II within a 1.2 cM confidence interval of marker Ps900203 (Duarte et al, 2014;Coyne et al, 2019). The genomic sequence of the Fsp-Ps2.1 ± 1.2 cM (±201,800 nt) was obtained from the pea genome (Kreplak et al, 2019).…”

Section: Functional Annotation Of Qtl Associated With Fsp Tolerance Imentioning

confidence: 99%

“…Recent studies conducted under controlled conditions have reported three QTLs; QTL Fsp-Ps 2.1 explains 44.4–53.4% of the phenotypic variance within a 1.2 cM confidence interval. The other two QTLs, Fsp-Ps 3.2 , and Fsp-Ps 3.3 explain 3.6–4.6% of the phenotypic variance related to Fsp root rot tolerance ( Coyne et al, 2015 , 2019 ). While the genes underlying these QTLs have not yet been identified, there is a reason for optimism given the recent release of the pea reference genome ( Kreplak et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Fusarium solani f. sp. pisi (Fsp) Responsive Genes in Pisum sativum

et al. 2020

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Pisum sativum (pea) is rapidly emerging as an inexpensive and significant contributor to the plant-derived protein market. Due to its nitrogen-fixation capability, short life cycle, and low water usage, pea is a useful cover-and-break crop that requires minimal external inputs. It is critical for sustainable agriculture and indispensable for future food security. Root rot in pea, caused by the fungal pathogen Fusarium solani f. sp. pisi (Fsp), can result in a 15-60% reduction in yield. It is urgent to understand the molecular basis of Fsp interaction in pea to develop root rot tolerant cultivars. A complementary genetics and gene expression approach was undertaken in this study to identify Fsp-responsive genes in four tolerant and four susceptible pea genotypes. Time course RNAseq was performed on both sets of genotypes after the Fsp challenge. Analysis of the transcriptome data resulted in the identification of 42,905 differentially expressed contigs (DECs). Interestingly, the vast majority of DECs were overexpressed in the susceptible genotypes at all sampling time points, rather than in the tolerant genotypes. Gene expression and GO enrichment analyses revealed genes coding for receptor-mediated endocytosis, sugar transporters, salicylic acid synthesis, and signaling, and cell death were overexpressed in the susceptible genotypes. In the tolerant genotypes, genes involved in exocytosis, and secretion by cell, the anthocyanin synthesis pathway, as well as the DRR230 gene, a pathogenesis-related (PR) gene, were overexpressed. The complementary genetic and RNAseq approach has yielded a set of potential genes that could be targeted for improved tolerance against root rot in P. sativum. Fsp challenge produced a futile transcriptomic response in the susceptible genotypes. This type of response is hypothesized to be related to the speed at which the pathogen infestation advances in the susceptible genotypes and the preexisting level of disease-preparedness in the tolerant genotypes.

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Section: Discussionmentioning

confidence: 86%

Section: Pathogenesis-related (Pr) Genesmentioning

confidence: 89%

Section: Functional Annotation Of Qtl Associated With Fsp Tolerance Imentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of Fusarium solani f. sp. pisi (Fsp) Responsive Genes in Pisum sativum

et al. 2020

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“…A strong QTL, Fsp-Ps 2.1 , governing resistance to Fsp has been detected in the recombinant inbred line (RIL) populations of pea (Baccara × PI 180693). The QTL Fsp-Ps 2.1 has been identified along with two other minor variance QTLs using three criteria: root disease severity, ratios of diseased vs. healthy shoot heights, and dry plant weights under controlled conditions (Coyne et al, 2019).…”

Section: Using Genomics To Understand the Basics Of Plant–pathogen Inmentioning

confidence: 99%

Genomics of Plant Disease Resistance in Legumes

2019

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The constant interactions between plants and pathogens in the environment and the resulting outcomes are of significant importance for agriculture and agricultural scientists. Disease resistance genes in plant cultivars can break down in the field due to the evolution of pathogens under high selection pressure. Thus, the protection of crop plants against pathogens is a continuous arms race. Like any other type of crop plant, legumes are susceptible to many pathogens. The dawn of the genomic era, in which high-throughput and cost-effective genomic tools have become available, has revolutionized our understanding of the complex interactions between legumes and pathogens. Genomic tools have enabled a global view of transcriptome changes during these interactions, from which several key players in both the resistant and susceptible interactions have been identified. This review summarizes some of the large-scale genomic studies that have clarified the host transcriptional changes during interactions between legumes and their plant pathogens while highlighting some of the molecular breeding tools that are available to introgress the traits into breeding programs. These studies provide valuable insights into the molecular basis of different levels of host defenses in resistant and susceptible interactions.

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Evaluation of Pea Accessions Differing in Flower and Seed Coat Pigmentation for Resistance to Fusarium avenaceum Root Rot

Awodele,

Gali,

Sivachandra Kumar

et al. 2024

Legume Science

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Pea production across the world is significantly limited by root rot disease, which is caused by many fungal and oomycetes pathogens. In Canada, Fusarium avenaceum is the most devastating pathogen of the Fusarium root rot complex of pea. Host genetic resistance is the most effective control method for this disease. Evaluation of global pea accessions and Canadian varieties for F. avenaceum root rot resistance has not been reported to date. This study evaluated 20 pea accessions of different market classes with pigmented or nonpigmented seed coats and flowers for F. avenaceum resistance under controlled conditions. The pea accessions CDC Acer, CDC Vienna, PBA OURA, Morgan, CDC Blazer, CDC Dakota, and PI 280609, which have pigmented flowers and seed coats, were identified as resistant or partially resistant to F. avenaceum. This was based on their root rot severity scores and ability to tolerate F. avenaceum infection without significant (p > 0.05) reductions in plant height, shoot dry weight, and root dry weight. Among the varieties with nonpigmented flowers and seed coats, only Cameor showed partial resistance to F. avenaceum when challenged with reduced conidial concentration. Root dry weight (R = −0.86), plant height (R = −0.82), and shoot dry weight (R = −0.78) had a strong negative correlation (p < 0.001) with disease severity, suggesting that F. avenaceum root rot can negatively impact the growth and development of pea seedlings. F. avenaceum resistance identified in this study can be utilized to study the molecular basis of the resistance and develop disease‐resistant varieties. While our findings suggest a relationship between pigmentation and F. avenaceum resistance, future research with a larger, more diverse panel is warranted to validate these initial results.

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Confirmation of Fusarium root rot resistance QTL Fsp-Ps 2.1 of pea under controlled conditions

Cited by 30 publications

References 44 publications

Identification of Fusarium solani f. sp. pisi (Fsp) Responsive Genes in Pisum sativum

Identification of Fusarium solani f. sp. pisi (Fsp) Responsive Genes in Pisum sativum

Genomics of Plant Disease Resistance in Legumes

Evaluation of Pea Accessions Differing in Flower and Seed Coat Pigmentation for Resistance to Fusarium avenaceum Root Rot

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