QTL analysis of durable stripe rust resistance in the North American winter wheat cultivar Skiles

Liu, L.; Yuan, Congying; Wang, M. N.; See, Deven R.; Zemetra, Robert S.; Chen, X. M.

doi:10.1007/s00122-019-03307-2

Cited by 34 publications

(59 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DH lines with more QTL showed higher resistance, demonstrating that pyramiding multiple HTAP QTL each with partial resistance can improve the level of resistance, even to the high level in PI 197734, without producing any uredinia. The results agree with previous studies that combining different genes for HTAP resistance can achieve high level, durable resistance to stripe rust (Liu et al, 2018(Liu et al, , 2019bLiu L. et al, 2020;Mu et al, 2020).…”

Section: Discussionsupporting

confidence: 92%

“…Haplotypes of two or more markers can greatly improve the accuracy for MAS. The haplotype approach has been used in previous studies to indicate the presence and absence of resistance genes or QTL (Feng et al, 2015(Feng et al, , 2018Xiang et al, 2016;Liu et al, 2018Liu et al, , 2019bLiu Y. et al, 2020). In the present study, we genotyped 95 spring wheat cultivars and breeding lines developed and grown in the U.S. Pacific Northwest with three KASP markers for YrQPI197734-1A and four KASP markers for YrQPI197734-3B converted from the GMS-SNP markers within the QTL regions (Supplementary Table S3).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mapping Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat PI 197734 Using a Doubled Haploid Population and Genotyping by Multiplexed Sequencing

et al. 2020

View full text Add to dashboard Cite

Stripe rust caused by Puccinia striiformis f. sp. tritici ( Pst ) is a global concern for wheat production. Spring wheat cultivar PI 197734, of Sweden origin, has shown high-temperature adult-plant resistance (APR) to stripe rust for many years. To map resistance quantitative trait loci (QTL), 178 doubled haploid lines were developed from a cross of PI 197734 with susceptible AvS. The DH lines and parents were tested in fields in 2017 and 2018 under natural infection of Pst and genotyped with genotyping by multiplexed sequencing (GMS). Kompetitive allele specific PCR (KASP) and simple sequence repeat (SSR) markers from specific chromosomal regions were also used to genotype the population to validate and saturate resistance QTL regions. Two major QTL on chromosomes 1AL and 3BL and one minor QTL on 2AL were identified. The two major QTL, QYrPI197734.wgp-1A and QYrPI197734.wgp-3B , were detected in all tested environments explaining up to 20.7 and 46.8% phenotypic variation, respectively. An awnletted gene mapped to the expected distal end of chromosome 5AL indicated the accuracy of linkage mapping. The KASP markers converted from the GMS-SNPs in the 1A and 3B QTL regions were used to genotype 95 US spring wheat cultivars and breeding lines, and they individually showed different percentages of polymorphisms. The haplotypes of the three markers for the 1A QTL and four markers for the 3B QTL identified 37.9 and 21.1% of the wheat cultivar/breeding lines possibly carrying these two QTL, indicating their usefulness in marker-assisted selection (MAS) for incorporating the two major QTL into new wheat cultivars.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Mapping Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat PI 197734 Using a Doubled Haploid Population and Genotyping by Multiplexed Sequencing

et al. 2020

View full text Add to dashboard Cite

show abstract

“…To overcome the drawback of GWAS unable to detect rare alleles, we also used molecular markers for 18 important genes or QTL for stripe rust resistance to test the accessions. A total of 31 simple-sequence repeat (SSR), sequence-tagged site (STS), or kompetitive allele specific PCR (KASP) markers were used for determining the presence or absence of the following previously reported 18 Yr (yellow rust) genes or QTL in the accessions: Yr5 ( Marchal et al., 2018 ), Yr9 ( Mago et al., 2002 ), Yr10 ( Bariana et al., 2002 ), Yr15 ( Ramirez-Gonzalez et al., 2015 ), Yr17 ( Helguera et al., 2003 ), Yr18 ( Lagudah et al., 2009 ), Yr27 ( Chhetri et al., 2017 ), Yr30 ( Spielmeyer et al., 2003 ), Yr46 ( Forrest et al., 2014 ), Yr76 ( Xiang et al., 2016 ), Yr78 ( Dong et al., 2017 ), YrSP ( Feng et al., 2015 ), YrTr1 (X. M. Chen and associates, unpublished), QYrMa.wgp-1AS ( Liu et al., 2018 ), QYrel.wgp-2BS ( Liu et al., 2019a ), QYrsk.wgp-3BS ( Liu et al., 2019b ), QYrsk.wgp-4BL ( Liu et al., 2019b ), and QYr.wpg-1B.1 ( Naruoka et al., 2015 ). DNA samples from spring wheat lines carrying specific Yr genes, which were used in the previous GWAS study with spring wheat ( Liu et al., 2020 ), were included as positive controls for the genotyping with molecular markers, but their data were excluded from the data analysis of the winter wheat panel.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Association Study and Gene Specific Markers Identified 51 Genes or QTL for Resistance to Stripe Rust in U.S. Winter Wheat Cultivars and Breeding Lines

Lü

Liu

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

Stripe (yellow) rust, caused by fungal pathogen Puccinia striiformis f. sp. tritici ( Pst ), is a serious disease of wheat in the United States and many other countries. Growing resistant cultivars has been approved to be the best approach for control of stripe rust. To determine stripe rust resistance genes in U.S. winter wheat cultivars and breeding lines, we analyzed a winter wheat panel of 857 cultivars and breeding lines in a genome-wide association study (GWAS) using genotyping by multiplexed sequencing (GMS) and by genotyping with molecular markers of 18 important stripe rust resistance genes or quantitative trait loci (QTL). The accessions were phenotyped for stripe rust response at adult-plant stage under natural infection in Pullman and Mount Vernon, Washington in 2018 and 2019, and in the seedling stage with six predominant or most virulent races of Pst . A total of 51 loci were identified to be related to stripe rust resistance, and at least 10 of them ( QYrww.wgp.1D-3 , QYrww.wgp.2B-2 , QYrww.wgp.2B-3 , QYrww.wgp.2B-4 , QYrww.wgp.3A , QYrww.wgp.5A , QYrww.wgp.5B , QYrww.wgp.5D , QYrww.wgp.6A-2 and QYrww.wgp.7B-3 ) were previously reported. These genes or QTL were found to be present at different frequencies in breeding lines and cultivars developed by breeding programs in various winter wheat growing regions. Both Yr5 and Yr15 , which are highly resistant to all races identified thus far in the U.S., as well as Yr46 providing resistance to many races, were found absent in the breeding lines and commercially grown cultivars. The identified genes or QTL and their markers are useful in breeding programs to improve the level and durability of resistance to stripe rust.

show abstract

“…These two major diseases can dramatically reduce crop yield during prevalent years (Bokore et al, 2017). Numerous powdery mildew and stripe rust resistance genes have been identified in wheat and its relatives, and these genes have played a fundamental role in wheat breeding (Liu et al, 2019). Nevertheless, most resistance genes are quickly overcome due to rapid virulence changes in pathogen populations (Zhan et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic Analysis and Molecular Marker Development for a New Wheat–Thinopyrum ponticum 1Js (1D) Disomic Substitution Line With Resistance to Stripe Rust and Powdery Mildew

et al. 2020

View full text Add to dashboard Cite

Thinopyrum ponticum (2n = 10x = 70), a member of the tertiary gene pool of wheat (Triticum aestivum L.), harbors many biotic and abiotic stress resistance genes. CH10A5, a novel disomic substitution line from a cross of T. aestivum cv. 7182 and Th. ponticum, was characterized by cytogenetic identification, in situ hybridization, molecular marker analysis, and morphological investigation of agronomic traits and disease resistance. Cytological observations showed that CH10A5 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. Genome in situ hybridization (GISH) analysis indicated that two of its chromosomes came from the J s genome of Th. ponticum, and wheat 15K array mapping and fluorescence in situ hybridization (FISH) revealed that chromosome 1D was absent from CH10A5. Polymorphic analysis of molecular markers indicated that the pair of alien chromosomes belonged to homoeologous group one, designated as 1J s. Thus, CH10A5 was a wheat-Th. ponticum 1J s (1D) disomic substitution line. Field disease resistance trials demonstrated that the introduced Th. ponticum chromosome 1J s was probably responsible for resistance to both stripe rust and powdery mildew at the adult stage. Based on specific-locus amplified fragment sequencing (SLAF-seq), 507 STS molecular markers were developed to distinguish chromosome 1J s genetic material from that of wheat. Of these, 49 STS markers could be used to specifically identify the genetic material of Th. ponticum. CH10A5 will increase the resistance gene diversity of wheat breeding materials, and the markers developed here will permit further tracing of heterosomal chromosome fragments in the future.

show abstract

QTL analysis of durable stripe rust resistance in the North American winter wheat cultivar Skiles

Cited by 34 publications

References 43 publications

Mapping Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat PI 197734 Using a Doubled Haploid Population and Genotyping by Multiplexed Sequencing

Mapping Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat PI 197734 Using a Doubled Haploid Population and Genotyping by Multiplexed Sequencing

Genome-Wide Association Study and Gene Specific Markers Identified 51 Genes or QTL for Resistance to Stripe Rust in U.S. Winter Wheat Cultivars and Breeding Lines

Cytogenetic Analysis and Molecular Marker Development for a New Wheat–Thinopyrum ponticum 1Js (1D) Disomic Substitution Line With Resistance to Stripe Rust and Powdery Mildew

Contact Info

Product

Resources

About