Mapping Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat PI 197734 Using a Doubled Haploid Population and Genotyping by Multiplexed Sequencing

Liu, Lu; Yuan, Congying; Wang, Meinan; See, Deven R.; Chen, Xianming

doi:10.3389/fpls.2020.596962

Cited by 7 publications

(5 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liu et al. (2020) attributed 7.34–11.36% of the phenotypic variance to QYrPI197734.wgp‐2A , which is also comparable to QYr.lrdc‐2A.2 explaining 5.5% of the phenotypic variance.…”

Section: Discussionmentioning

confidence: 93%

“…The long arm of chromosome 2A has always been a major source of stripe rust resistance (Bokore et al., 2017; Vazquez et al., 2015; Wang et al., 2019). Furthermore, minor effect QTL on chromosome arm 2AL have been reported in wheat cultivars Recital (Dedryver et al., 2009), Taldor (Paillard et al., 2012), Kundan (Ren et al., 2017), Chuanmai 55 (Yang et al., 2019), and PI 197734 (Liu et al., 2020). According to genetic maps produced by Wang et al.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

QTL mapping for adult plant field resistance to stripe rust in the AAC Cameron/P2711 spring wheat population

et al. 2022

View full text Add to dashboard Cite

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating disease of wheat (Triticum aestivum L.). Deployment of resistant wheat cultivars is one of the best strategies to mitigate disease-associated risks. The genotype P2711 possesses effective stripe rust resistance under field conditions in western Canada, however, the genetic basis of this resistance is unknown. To identify resistance, a recombinant inbred line (RIL) population comprised of 252 RILs was developed from the cross AAC Cameron/P2711. This population was evaluated for stripe rust severity at the adult plant stage in Creston, BC (in 2018, 2019, and 2020) and Lethbridge, AB (in 2018 and 2020), and was genotyped using the wheat 90K iSelect single nucleotide polymorphism (SNP) assay. A high-density genetic map comprised of 8,915 markers was constructed covering all the wheat chromosomes. Four resistance quantitative trait loci (QTLs) were contributed by P2711 and three from AAC Cameron. QYr.lrdc-2A.1, corresponding to the Yr17 gene, was the most stable QTL and detected in four out of five environments, whereas QYr.lrdc-2B was the second most stable QTL. These two QTL along with QYr.lrdc-5A showed a significant reduction in stripe rust severity when present together. Where most of the QTLs detected in both locations, QYr.lrdc-1A.1 and QYr.lrdc-1A.2, both were detected only in Creston, BC. Stable QTLs on chromosome 2A, 2B, and 5A, and their closely associated markers identified in this study could be utilized in marker-assisted selection for stripe rust resistant cultivar development.

show abstract

“…Liu et al. (2020) attributed 7.34–11.36% of the phenotypic variance to QYrPI197734.wgp‐2A , which is also comparable to QYr.lrdc‐2A.2 explaining 5.5% of the phenotypic variance.…”

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

QTL mapping for adult plant field resistance to stripe rust in the AAC Cameron/P2711 spring wheat population

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The SP-SNP markers identified in the present study, together with the other nearly 100 SP-SNP markers associated with Pst virulence in previous studies [27,31,35,37,44], will be converted to KASP markers for further confirmation of the association of the SP-SNP markers with their avirulence genes and also for establishing a set of virulence-related markers for monitoring changes in both virulence and molecular genotypes in the Pst population. Compared to SNP genotyping, KASP markers are relatively cheap and easy to use, as demonstrated in our program for stripe rust resistance genes in wheat [53][54][55][56][57]. The present study provides genomic resources for further marker development and research on host-pathogen interactions, as well as pathogen population dynamics.…”

Section: Discussionmentioning

confidence: 95%

Identification of Secreted Protein Gene-Based SNP Markers Associated with Virulence Phenotypes of Puccinia striiformis f. sp. tritici, the Wheat Stripe Rust Pathogen

Bai

Wang

Xia

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a destructive disease that occurs throughout the major wheat-growing regions of the world. This pathogen is highly variable due to the capacity of virulent races to undergo rapid changes in order to circumvent resistance in wheat cultivars and genotypes and to adapt to different environments. Intensive efforts have been made to study the genetics of wheat resistance to this disease; however, no known avirulence genes have been molecularly identified in Pst so far. To identify molecular markers for avirulence genes, a Pst panel of 157 selected isolates representing 126 races with diverse virulence spectra was genotyped using 209 secreted protein gene-based single nucleotide polymorphism (SP-SNP) markers via association analysis. Nineteen SP-SNP markers were identified for significant associations with 12 avirulence genes: AvYr1, AvYr6, AvYr7, AvYr9, AvYr10, AvYr24, AvYr27, AvYr32, AvYr43, AvYr44, AvYrSP, and AvYr76. Some SP-SNPs were associated with two or more avirulence genes. These results further confirmed that association analysis in combination with SP-SNP markers is a powerful tool for identifying markers for avirulence genes. This study provides genomic resources for further studies on the cloning of avirulence genes, understanding the mechanisms of host–pathogen interactions, and developing functional markers for tagging specific virulence genes and race groups.

show abstract

“…As both Yr29 and QYrZM9023.swust-1BL confer APR, QYrZM9023.swust-1BL should be Yr29 . Yr29 has been reported and deployed widely in wheat varieties around the world ( William et al., 2006 ; Melichar et al., 2008 ; Lan et al., 2014 ; Hou et al., 2015 ; Maccaferri et al., 2015 ; Muleta et al., 2017 ; Wang and Chen, 2017 ; Cobo et al., 2018 ; Godoy et al., 2018 ; Liu et al., 2019 ; Rosa et al., 2019 ; Liu et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, races virulent on Yr5 gene have been reported in Australia, India, China, and Turkey ( Wellings and McIntosh, 1990 ; Zhang et al., 2020 ; Tekin et al., 2021 ), and the Yr15 virulence has been documented in Afghanistan ( Gerechter-Amitai et al., 1989 ). Most wheat cultivars that have shown durable resistance to stripe rust have APR or HTAP resistance controlled by variable numbers of genes or QTL ( Lin and Chen, 2007 ; Lin and Chen, 2008b ; Santra et al., 2008 ; Carter et al., 2009 ; Paillard et al., 2012 ; Ren et al., 2012a ; Lu et al., 2014 ; Zhou et al., 2014 ; Dong et al., 2017 ; Feng et al., 2018 ; Liu et al., 2018 ; Liu et al., 2019 ; Liu et al., 2020 ). In order to obtain a high degree of durable resistance, combining the two types of resistance types in the same background is considered a preferred method to improve the resistance to stripe rust in wheat breeding ( Chen, 2007 ; Ren et al., 2012a ; Chen, 2013 ; Liu et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide QTL mapping for stripe rust resistance in spring wheat line PI 660122 using the Wheat 15K SNP array

Yan,

Jia,

Tan

et al. 2023

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

IntroductionStripe rust is a global disease of wheat. Identification of new resistance genes is key to developing and growing resistant varieties for control of the disease. Wheat line PI 660122 has exhibited a high level of stripe rust resistance for over a decade. However, the genetics of stripe rust resistance in this line has not been studied. A set of 239 recombinant inbred lines (RILs) was developed from a cross between PI 660122 and an elite Chinese cultivar Zhengmai 9023.MethodsThe RIL population was phenotyped for stripe rust response in three field environments and genotyped with the Wheat 15K single-nucleotide polymorphism (SNP) array.ResultsA total of nine quantitative trait loci (QTLs) for stripe rust resistance were mapped to chromosomes 1B (one QTL), 2B (one QTL), 4B (two QTLs), 4D (two QTLs), 6A (one QTL), 6D (one QTL), and 7D (one QTL), of which seven QTLs were stable and designated as QYrPI660122.swust-4BS, QYrPI660122.swust-4BL, QYrPI660122.swust-4DS, QYrPI660122.swust-4DL, QYrZM9023.swust-6AS, QYrZM9023.swust-6DS, and QYrPI660122.swust-7DS. QYrPI660122.swust-4DS was a major all-stage resistance QTL explaining the highest percentage (10.67%–20.97%) of the total phenotypic variation and was mapped to a 12.15-cM interval flanked by SNP markers AX-110046962 and AX-111093894 on chromosome 4DS.DiscussionThe QTL and their linked SNP markers in this study can be used in wheat breeding to improve resistance to stripe rust. In addition, 26 lines were selected based on stripe rust resistance and agronomic traits in the field for further selection and release of new cultivars.

show abstract

Mapping Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat PI 197734 Using a Doubled Haploid Population and Genotyping by Multiplexed Sequencing

Cited by 7 publications

References 51 publications

QTL mapping for adult plant field resistance to stripe rust in the AAC Cameron/P2711 spring wheat population

QTL mapping for adult plant field resistance to stripe rust in the AAC Cameron/P2711 spring wheat population

Identification of Secreted Protein Gene-Based SNP Markers Associated with Virulence Phenotypes of Puccinia striiformis f. sp. tritici, the Wheat Stripe Rust Pathogen

Genome-wide QTL mapping for stripe rust resistance in spring wheat line PI 660122 using the Wheat 15K SNP array

Contact Info

Product

Resources

About