QCM-based rapid detection of PCR amplification products of Ehrlichia canis

Bunroddith, Kespunyavee; Viseshakul, Nareerat; Chansiri, Kosum; Lieberzeit, Peter A.

doi:10.1016/j.aca.2017.10.037

Cited by 39 publications

(27 citation statements)

References 28 publications

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“…PNAs are DNA mimics in which the bases are attached to a neutral N-(2-aminoethyl)-glycine pseudopeptide backbone, and LNAs are nucleic acid analogues in which the ribose ring is "locked" by a methylene bridge that shows increased thermal stability [4]. Other capture probes used are chemically modified at one of the oligonucleotide ends, and usually, biotin, thiol, or amine residues are aggregated, biotin and thiol being the most used chemical modifications [7][8][9][37][38][39][40]. Moreover, the surface chemistry used in this study enabled the immobilization of cDNA, which allowed developing a biosensor for gene expression determination from children with obesity.…”

Section: Real-time Quantitative Polymerase Chain Reactionmentioning

confidence: 99%

“…Song et al measured in real-time human papillomavirus 16 DNA through a loopmediated isothermal amplification-QCM biosensor [7]. Bunroddith and colleagues developed a QCM sensor for the ultrasensitive detection of DNA with results comparable to the ones obtained using a SPR sensor platform [8]. Ravi et al reported a rapid screening method of Ehrlichia canis PCR amplification products based on PCR assay combined with QCM [9].…”

Section: Introductionmentioning

confidence: 99%

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NLRP3, IL-1β, and Caspase-1 Gene Transcript Identification and Expression by QCM-D in Obese Children

et al. 2019

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Background. Obesity in children is highly prevalent in Mexican population. Adipose tissue has been related to specific pro- and anti-inflammatory cytokine and inflammasome gene and protein expression patterns. Actually, there is no existing biosensor for detecting gene expression patterns in children with obesity. The quartz crystal microbalance with dissipation monitoring (QCM-D) has been used as a transducer for DNA biosensor design. Results. In this study, the gene expression pattern of IL-1β, NLRP3, and CASPASE-1 in children with obesity was successfully determined by means of QCM-D. Gene expression patterns were validated with those obtained by quantitative polymerase chain reaction (qPCR), a validated molecular biology technique for gene expression quantification. QCM-D analysis of the detected mass corresponding results for each of the genes showed a major detected mass for IL-1β, followed by similar NLRP3 and constitutive gene 18S deposited mass and a smaller deposited mass for CASPASE-1. Surprisingly, when comparing mRNA gene expression results for NLRP3, IL-1β, and CASPASE-1 obtained with qPCR and QCM-D, similar patterns were found, revealing greatest expression of IL-1β, followed by NLRP3, with CASPASE-1 being the molecule of least expression in the group of children with obesity. AFM images illustrate the step-by-step changes that took place on the quartz surface. Conclusions. QCM-D proved successfully for determining the gene transcripts and expression of NLRP3, IL-1β, and CASPASE-1 in children with obesity, with similar results validated by qPCR. “QCM-D decreases detection costs compared with a validated molecular biology technique.” The QCM-D biosensor developed by our group was successful for gene expression determination; in the future, it can be used for molecular diagnosis.

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Section: Real-time Quantitative Polymerase Chain Reactionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NLRP3, IL-1β, and Caspase-1 Gene Transcript Identification and Expression by QCM-D in Obese Children

et al. 2019

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“…Probe hybridization techniques include northern blotting, in situ hybridization, and microarrays are well established but often require a large quantity of samples. Amplification technologies, including real‐time fluorescent quantitative polymerase chain reaction (RT‐PCR) and rolling circle amplification (RCA), are often time‐consuming and complicated to operate. Recently, the quartz crystal microbalance (QCM) mass sensor has the characteristics of high sensitivity, label‐free and, real‐time analysis, and it has been widely used in biosensors .…”

Section: Introductionmentioning

confidence: 99%

“…Amplification technologies, including real‐time fluorescent quantitative polymerase chain reaction (RT‐PCR) and rolling circle amplification (RCA), are often time‐consuming and complicated to operate. Recently, the quartz crystal microbalance (QCM) mass sensor has the characteristics of high sensitivity, label‐free and, real‐time analysis, and it has been widely used in biosensors . Many studies have shown that DNA‐modified QCM biosensors can be applied to detect such processes as DNA hybridization and single base mismatch of DNA .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the quartz crystal microbalance (QCM) mass sensor has the characteristics of high sensitivity, label-free and, real-time analysis, and it has been widely used in biosensors. [20][21][22][23][24][25] Many studies have shown that DNA-modified QCM biosensors can be applied to detect such processes as DNA hybridization 26 and single base mismatch of DNA. 27 However, studies on QCM biosensor for miRNA detection has been still limited.…”

Section: Introductionmentioning

confidence: 99%

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Assembly of a miRNA‐modified QCM sensor for miRNA recognition through response patterns

Zheng

et al. 2018

J of Molecular Recognition

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In this paper, a miRNA‐based quartz crystal microbalance (QCM) biosensor was fabricated and used to the rapid and effective sensing of miRNA. The specific hybridization between probe miRNA and different selected miRNAs (miR‐27a, miR‐27b, and Let‐7a) cause a different interaction mode, thus display different frequency change and response patterns in the QCM sensor, which were used to detect miR‐27a and miR‐27b. The selective sensing of miR‐27a in mixed miRNA solution was also achieved. This miRNA‐based QCM biosensor has the advantages of real‐time, label‐free, and short cycle detection.

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The Effect of (3‐Mercaptopropyl)trimethoxysilane (MPS) Coating on the Genetic Detection Performance of Quartz Crystal Microbalance‐Dissipation (QCM‐D) Biosensor: Novel Intact Double‐Layered Surface Modification on QCM‐D

Soysaldı

Soylu

2021

ChemistrySelect

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Biosensor's ability depends on both platform performance and surface modification. In mass sensitive biosensors, a stable coating with molecular structural dynamism increases sensitivity. Also, its orientation and smoothness directly affect the performance of cross-linker molecules and receptors. In this study, the performance of intact double-layered (ID) coating used on QCMÀ D for the first time in DNA detection of MPS was investigated. The sensitivity of the QCMÀ D with ID-MPS was compared to the uncoated. At the concentrations of 10 10 and 10 13 copies/mL, the method with ID-MPS was approximately 6.4 times more sensitive. A shift of 1.22(� 0.42) Hz was observed at the detection limit at 10 8 copies/mL, while it was 0.19(� 0.05) Hz in the negative control. Thus, a signal-to-noise-ratio greater than 3, which is a measure of signal quality in biosensors, was exceeded with a ratio of 3.33, and it was shown that the detection signal at the sensitivity limit was sufficient. In addition, the two methods were compared visually with atomic force microscopy in terms of the amount and distribution of captured molecules, and it was determined that the ID-MPS was more efficient.

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QCM-based rapid detection of PCR amplification products of Ehrlichia canis

Cited by 39 publications

References 28 publications

NLRP3, IL-1β, and Caspase-1 Gene Transcript Identification and Expression by QCM-D in Obese Children

NLRP3, IL-1β, and Caspase-1 Gene Transcript Identification and Expression by QCM-D in Obese Children

Assembly of a miRNA‐modified QCM sensor for miRNA recognition through response patterns

The Effect of (3‐Mercaptopropyl)trimethoxysilane (MPS) Coating on the Genetic Detection Performance of Quartz Crystal Microbalance‐Dissipation (QCM‐D) Biosensor: Novel Intact Double‐Layered Surface Modification on QCM‐D

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