Peroxiredoxin 2 (PRDX2) has been known to act as an antioxidant enzyme whose main function is H(2)O(2) reduction in cells. We aimed to study the expression patterns of PRDX2 in mouse ovaries and explore the function of this protein in apoptosis of granulosa cells (GCs). We found that the expression of the PRDX2 protein in atretic follicle GCs was markedly higher than in healthy follicle GCs. In vitro, the transfection of siRNA targeting the Prdx2 gene inhibited the proliferation and induced the apoptosis of primary cultured GCs. Furthermore, suppression of PRDX2 resulted in the augmentation of endogenous H(2)O(2), and the ability to eliminate the exogenous H(2)O(2) was attenuated. The expression of PRDX2 and nuclear factor kappa-light-chain-enhancer of activated B cells (NFKB), whose activity was inhibited by binding to IKB, increased in GCs treated with various concentrations of H(2)O(2) for 30 min. However, no significant change in cytoplasmic IKB expression was observed. At 2 h after treatment with H(2)O(2), nuclear NFKB expression level was reduced, cytoplasmic IKB expression was increased, and PRDX2 expression was unchanged. Silencing of the Prdx2 gene caused early changes in NFKB and IKB expression in the primary cultured GCs compared to that in control cells. Taken together, these data suggest that PRDX2 plays an important role in inhibiting apoptosis in GCs and that PRDX2 actions may be related to the expression of NFKB and IKB.
Oxo-anion
pollutants pose a serious threat to natural water systems.
It is highly necessary to develop cationic porous materials capable
of removing oxo-anion from sewage. However, they are relatively rarely
reported compared with anionic and neutral materials. Herein, two
cationic amorphous metal–organic cage-based solids containing
cages of different sizes, namely, aMOC-1 and aMOC-2, were constructed and use for removing oxo-anions
from water. Because the cages in these materials have large cavities
(with about 3.5 and 4.5 nm in diameter for aMOC-1
and aMOC-2, respectively) and high positive charges
(positive +24 for both), both aMOC-1 and aMOC-2 exhibit rapid kinetics in carcinogenic chromate (CrO4
2– and Cr2O7
2–) and ReO4
– (a surrogate anion of radioactive
isotope TcO4
–) adsorption. The adsorption
equilibrium can be reached within only a few minutes. The adsorption
capacities of aMOC-1 for CrO4
2–, Cr2O7
2–, and ReO4
– are 157.4, 203.8, and 350 mg/g, respectively.
Because aMOC-2 contains larger metal–organic
cage, the corresponding adsorption capacities are 242.0, 407.0, and
583.9 mg/g, respectively. The capacity of aMOC-2
for Cr(VI) oxo-anion adsorption ranks the highest among all related
materials ever reported. In addition, the oxo-anions can be released
rapidly within several minutes from the oxo-anion-loaded aMOCs in 2 M NaNO3 solution, which allows these materials
to exhibit good reusability. Finally, the aMOCs have
a potential application in the removal of Cr(VI) from electroplating
bath wastewater, in which the concentration changes from 10 to 0.17
ppm after treatment with aMOCs.
Background
Heterotopic pregnancy occurred after frozen embryo transfer with two D3 embryos, and the case had a history of bilateral salpingectomy due to salpingocyesis. An ectopic heterotopic pregnancy was implanted in the left psoas major muscle, which has not been previously reported.
Case presentation
A 33-year-old woman presented with left back pain after curettage due to foetal arrest in the uterus without vaginal bleeding and spotting, and painkillers relieved the pain initially. When the painkillers ceased to work, the patient returned to the hospital. The β-human chorionic gonadotropin (β-hCG) level remained increased compared with the time of curettage, and a diagnosis of retroperitoneal abdominal pregnancy was suggested by ultrasonography and computerized tomography (CT) with the gestational sac implanted in the left psoas major muscle at the left hilum level. Laparotomy was performed to remove the ectopic pregnancy. During the operation, we carefully separated the adipose tissue between the space of the left kidney door and left psoas major muscle, peeled away the gestational sac that was approximately 50 mm × 40 mm with a 25-mm-long foetal bud, and gave a local injection of 10 mg of methotrexate in the psoas major muscle. Fifty days later, β-hCG decreased to normal levels.
Conclusion
It is necessary to pay more attention to the main complaints to exclude rare types of ectopic pregnancies of the pelvis and abdomen after embryo transfer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.