Q fever outbreak in the terraced vineyards of Lavaux, Switzerland

Bellini, Chiara; Magouras, Ioannis; Chapuis-Taillard, Caroline; Clerc, Olivier; Masserey, Éric; Peduto, G.; Péter, Olivier; Schaerrer, S.; Schuepbach, Gertraud; Greub, Gilbert

doi:10.1002/2052-2975.37

Cited by 4 publications

(4 citation statements)

References 37 publications

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“…The highest seroprevalence recorded in these areas may be due to the larger size of the flocks in these provinces as compared with the rest of Piedmont and the abundance of alpine pastures shared by different flocks. Of note is that about 60 to 80 animal infections per year and recurrent Q fever outbreaks in humans have been recorded in Switzerland since 2009, the latest occurring in spring 2012 in the Canton of Vaud with 14 human cases involved (Bellini et al, 2014). Moreover, at least four human outbreaks traced to exposure to sheep, goats, and cattle have been reported in France over the last 25 years (Frankel et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Q fever seroprevalence and risk factors in sheep and goats in northwest Italy

Rizzo

Vitale

Ballardini

et al. 2016

Preventive Veterinary Medicine

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Section: Discussionmentioning

confidence: 99%

“…Northwest Italy (Piedmont) is surrounded by the Alps on three sides and borders with France and Switzerland where outbreaks in both animals and humans have been reported (Frankel et al, 2011;Bellini et al, 2014) V., 2010, pp. 276), no other epidemiological data on Q fever prevalence in northwest Italy are available.…”

Section: Introductionmentioning

confidence: 99%

Q fever seroprevalence and risk factors in sheep and goats in northwest Italy

Rizzo

Vitale

Ballardini

et al. 2016

Preventive Veterinary Medicine

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“…According to Bellini et al , only 14% C. burnetii antibody positive (by ELISA) ruminants had C. burnetii DNA in the real time PCR assay [44]. However, Kargar et al .…”

Section: Resultsmentioning

confidence: 99%

Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants

Pradeep¹,

Spellman²,

Pratheesh³

et al. 2017

Vet World

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Background and Aim::In the course of our Indian Council of Medical Research project on coxiellosis in Puducherry and Tamil Nadu, 5.64% goat, 1.85% sheep, 1.06% buffaloes, and 0.97% cattle were positive for Coxiella burnetii antibodies by enzyme linked immunosorbent assay kit (IDEXX, Liebefeld, Switzerland). In this preliminary study, we have proceeded to look for C. burnetii DNA in those antibody positive specimens employing an imported commercial C. burnetii polymerase chain reaction (PCR) kit.Materials and Methods::Blood samples were collected during slaughtering. All 15 blood samples of antibody positive ruminants and three antibody negative samples were subjected to conventional Trans-PCR assay with a commercial PCR kit (Genekam Biotechnology AG, Duisburg, Germany). An in-house Trans-PCR was included in the study for comparison.Results::A total of 15 antibody positive and three antibody-negative serum samples belonging to 11 goat, 4 sheep, 1 cattle, and 2 buffaloes were tested in duplicate for the presence of C. burnetii DNA by the commercial agar gel PCR kit and an in-house Trans-PCR. Only one buffalo serum sample was positive for C. burnetii with a band at 243 bp in in-house Trans-PCR.Discussion::Seropositivity for C. burnetii need not necessarily translate into infectivity status of the animal. Conversely, seronegative ruminants can shed C. burnetii. Rapid disintegration of C. burnetii DNA during the storage period is an important impediment in QF-PCR research. This is the first time the performance of this commercial PCR kit is being validated in India.Conclusion::Commercial PCR kit, Genekam did not identify any positive sample, probably because it targeted a larger amplicon of 687 bp.

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“…Although a large number of animals are able to carry C. burnetii , the main reservoirs of the bacterium include sheep, goats and cattle (6). Q fever may cause outbreaks(7),(8), the largest to date having been registered in the Netherlands (9). The laboratory diagnosis of C. burnetii infections relies mainly on serology and molecular biology (10).…”

Section: Introductionmentioning

confidence: 99%

High Content Screening, a reliable system forCoxiella burnetiiisolation from clinical samples

Francis

Mioulane²,

Bideau

et al. 2019

Preprint

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24Q fever, caused by Coxiella burnetii, is a worldwide zoonotic disease that may cause severe 25 forms in humans and requires a specific and prolonged antibiotic treatment. Although the current 26 serological and molecular detection tools enable a reliable diagnosis of the disease, culture of C. 27 burnetii strains is mandatory to evaluate their antibiotic susceptibility and sequence their genome 28 in order to optimize patient management and epidemiological studies. However, cultivating this 29 fastidious microorganism is difficult and restricted to reference centers as it requires biosafety-30 level 3 laboratories and relies on cell culture performed by experienced technicians. In addition, 31 the culture yield is low, which results in a small number of isolates being available. In this work, 32 we developed a novel high content screening (HCS) isolation strategy based on optimized high-33 throughput cell culture and automated microscopic detection of infected cells with specifically-34 designed algorithms targeting cytopathic effects. This method was more efficient than the shell-35 vial assay when applied to both frozen specimens (7 isolates recovered by HCS only, sensitivity 36 91% vs 78% for shell-vial) and fresh samples (1 additional isolate using HCS, sensitivity 7% vs 37 5% for shell-vial). In addition, detecting positive cultures by an automated microscope reduced 38 the need for expertise and saved 24% of technician working time. Application of HCS to 39 antibiotic susceptibility testing of 12 strains demonstrated that it was as efficient as the standard 40 procedure that combines shell-vial culture and quantitative PCR. Overall, this high-throughput 41 HCS system paves the way to the development of improved cell culture isolation of human 42 viruses.43

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Q fever outbreak in the terraced vineyards of Lavaux, Switzerland

Cited by 4 publications

References 37 publications

Q fever seroprevalence and risk factors in sheep and goats in northwest Italy

Q fever seroprevalence and risk factors in sheep and goats in northwest Italy

Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants

High Content Screening, a reliable system forCoxiella burnetiiisolation from clinical samples

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