Q-Absorbance Ratio Spectrophotometric Method for the Simultaneous Estimation of Metformin Hydrochloride and Voglibose in Tablet Dosage form

Tejashree, R Kedar; Dashetwar, Anupama R.; Kardile, D P; Jadhav, Aruna P.; Bhagat, Vishwas C.; Shete, Rajkumar V.

doi:10.52711/0974-360x.2021.00554

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…13 Where Cx and Cy are the x and y concentrations, A is the sample's absorbance at iso-absorptive λmax, and a1 and a2 are the x and y absorptivity at iso-absorptive λmax, respectively. QM= A2 / A1 ………………………………..14 QX= a 𝑥 2/ a 𝑥 1 ………..……………………..15 Q y= ay2/ay1 …………………………………16 Where, A1 and A2 were absorbance of combination at λiso and λ max a𝑥 1 and ay1 are absorptivities of 𝑥 and y at λ iso a𝑥 2 and ay2 are absorptivities of 𝑥 and y at λ max [26][27][28]…”

Section: Q-absorbance Ratio Spectra Methodsmentioning

confidence: 99%

Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods

2023

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Four rapid, accurate and very simple derivative spectrophotometric techniques were developed for the quantitative determination of binary mixtures of estradiol (E2) and progesterone (PRG) formulated as a capsule. Method I is the first derivative zero-crossing technique, derivative amplitudes were detected at the zero-crossing wavelength of 239.27 and 292.51 nm for the quantification of estradiol and 249.19 nm for Progesterone. Method II is ratio subtraction, progesterone was determined at λmax 240 nm after subtraction of interference exerted by estradiol. Method III is modified amplitude subtraction, which was established using derivative spectroscopy and mathematical manipulations. Method IIII is the absorbance ratio technique, absorbance of both medicines was measured at two wavelengths λ1= 260, -absorptive point and λ2=240max of progesterone. The Q equations were used to calculate the final concentrations. The calibration curve is linear from 5.0–140 and 2.0–32.0 µg/ml for estradiol and progesterone respectively. The proposed techniques' selectivity was tested using synthetic combinations created in the lab and assessed using the standard addition method. Using one-way ANOVA, the outputs of the proposed ways were compared, and the result showed no significant differences between the proposed techniques.

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Section: Q-absorbance Ratio Spectra Methodsmentioning

confidence: 99%

Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods

2023

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“…Therefore, there is a need to develop a technique to simultaneously measure metformin hydrochloride and repaglinide in bulk and combined tablet dosage forms. An RP-HPLC method was developed for the simultaneous determination of metformin and repaglinide in bulk and composite tablet dosage forms [7][8][9][10][11][12][13][14][15]. The structures of metformin HCl and repaglinide are showed in Figures 1 and 2 respectively.…”

Section: Introductionmentioning

confidence: 99%

Novel RP-HPLC Method Development and Validation of Metformin HCl and Repaglinide in bulk and Tablet dosage form

Bhavyasri¹,

Begum²,

Sumakanth³

2023

Int J Pharm Phytopharmacol Res

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To quantify repaglinide and metformin HCl in largeand combination tablet dosage form simultaneously by RP-HPLC, a simple, precise, and accurate approach has been developed. The Chromatographic conditions employed were Agilent C18 column (150×4.6mm, 5µ) column using a Methanol: Water mixture in gradient mode as the mobile phase at a flow rate of 0.6 ml/min, λmax was found to be 245.5 nm in UV Spectrophotometer. The Retention time (Rt) of the Metformin HCl and Repaglinide was found to be 1.575 and 2.564min respectively. The established techniquewas confirmed as per ICH Q2 (R1) strategies. The method produces linear responses in the concentration range of 5:0.02-150:0.6 µg/ml for the RP-HPLC method. For the RP-HPLC procedure, a correlation coefficient value of 0.999 was found. As a result, the recomendedmethod may be employedto successfully quantify Metformin HCl and Repaglinide in bulk and combination tablet dosage form.

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“…A comprehensive review of the available literature found that a number of analytical techniques, such as spectrophotometric [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ], colorimetric [ 31 , 32 , 33 ], spectrofluorometric [ 33 , 34 , 35 ], and high-performance liquid chromatographic [ 36 , 37 , 38 , 39 ] methods, were described for the detection of linagliptin. It has been determined that colorimetric is more specific than spectrophotometry (UV region).…”

Section: Introductionmentioning

confidence: 99%

“…It has been determined that colorimetric is more specific than spectrophotometry (UV region). The methods used to analyse the LGN singly or in combination are cited in references [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ], which were based on the molecular absorption technique. These methods are sensitive, but they are not as selective as the method being studied.…”

Section: Introductionmentioning

confidence: 99%

Thermodynamic and Computational (DFT) Study of Non-Covalent Interaction Mechanisms of Charge Transfer Complex of Linagliptin with 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) and Chloranilic acid (CHA)

2022

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This study describes the non-covalent interactions of the charge transfer complex (CT), which was responsible for the synthesis of Linagliptin (LNG) with 2, 3-Dichloro-5, 6-Dicyano-1, 4-benzoquinone (DDQ), or with chloranilic acid (CHA) complexes in acetonitrile (MeCN) at temperatures of (25 ± 2 °C). Then, a UV–Vis spectrophotometer was utilized to identify Linagliptin (LNG) from these complexes. For the quantitative measurement of Linagliptin in bulk form, UV–Vis techniques have been developed and validated in accordance with ICH criteria for several aspects, including selectivity, linearity, accuracy, precision, LOD, LOQ, and robustness. The optimization of the complex synthesis was based on solvent polarization; the ratio of molecules in complexes; the association constant; and Gibbs energy (ΔG°). The experimental work is supported by the computational investigation of the complexes utilizing density functional theory as well as (QTAIM); (NCI) index; and (RDG). According to the optimized conditions, Beer’s law was observed between 2.5–100 and 5–100 µM with correlation coefficients of 1.9997 and 1.9998 for LGN-DDQ and LGN-CHA complexes, respectively. For LGN-DDQ and LGN-CHA complexes, the LOD and LOQ were (1.0844 and 1.4406 μM) and (3.2861 and 4.3655 μM), respectively. The approach was successfully used to measure LGN in its bulk form with high precision and accuracy.

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Q-Absorbance Ratio Spectrophotometric Method for the Simultaneous Estimation of Metformin Hydrochloride and Voglibose in Tablet Dosage form

Cited by 6 publications

References 14 publications

Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods

Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods

Novel RP-HPLC Method Development and Validation of Metformin HCl and Repaglinide in bulk and Tablet dosage form

Thermodynamic and Computational (DFT) Study of Non-Covalent Interaction Mechanisms of Charge Transfer Complex of Linagliptin with 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) and Chloranilic acid (CHA)

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