Pyruvatibacter mobilis gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122

Wang, Guanghua; Tang, Mingfang; Wu, Hao; Dai, Shikun; Li, Tao; Chen, Chenghao; He, Hui; Fan, Jing; Xiang, Wenzhou; Li, Xiang

doi:10.1099/ijsem.0.000692

Cited by 14 publications

(2 citation statements)

References 27 publications

(30 reference statements)

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“…As such, we relied on CFU counts, which avoid many of these difficulties but at the cost of media selectivity, as highlighted by the discrepancy between absence of CFUs in marine media and amplicon sequencing-based detection of some bacterial taxa. Literature perusal suggests that the most prevalent bacterial taxa detected in gnotobiotic anemones using sequencing (OTU0002, OTU0003, and OTU0006) cannot grow on our employed media and therefore escape CFU counting (Brooijmans et al, 2009;Yetti et al, 2015;Wang et al, 2016). Nevertheless, the antibiotic-treated anemones were highly bacteria depleted as more than 95% of the sequencing reads represented only three OTUs, which correspond to less than 5% of the active taxa detected in control anemones.…”

Section: Discussionmentioning

confidence: 94%

Surface Topography, Bacterial Carrying Capacity, and the Prospect of Microbiome Manipulation in the Sea Anemone Coral Model Aiptasia

et al. 2021

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Aiptasia is an emerging model organism to study cnidarian symbioses due to its taxonomic relatedness to other anthozoans such as stony corals and similarities of its microalgal and bacterial partners, complementing the existing Hydra (Hydrozoa) and Nematostella (Anthozoa) model systems. Despite the availability of studies characterizing the microbiomes of several natural Aiptasia populations and laboratory strains, knowledge on basic information, such as surface topography, bacterial carrying capacity, or the prospect of microbiome manipulation is lacking. Here we address these knowledge gaps. Our results show that the surface topographies of the model hydrozoan Hydra and anthozoans differ substantially, whereas the ultrastructural surface architecture of Aiptasia and stony corals is highly similar. Further, we determined a bacterial carrying capacity of ∼104 and ∼105 bacteria (i.e., colony forming units, CFUs) per polyp for aposymbiotic and symbiotic Aiptasia anemones, respectively, suggesting that the symbiotic status changes bacterial association/density. Microbiome transplants from Acropora humilis and Porites sp. to gnotobiotic Aiptasia showed that only a few foreign bacterial taxa were effective colonizers. Our results shed light on the putative difficulties of transplanting microbiomes between cnidarians in a manner that consistently changes microbial host association at large. At the same time, our study provides an avenue to identify bacterial taxa that exhibit broad ability to colonize different hosts as a starting point for cross-species microbiome manipulation. Our work is relevant in the context of microbial therapy (probiotics) and microbiome manipulation in corals and answers to the need of having cnidarian model systems to test the function of bacteria and their effect on holobiont biology. Taken together, we provide important foundation data to extend Aiptasia as a coral model for bacterial functional studies.

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Section: Discussionmentioning

confidence: 94%

Surface Topography, Bacterial Carrying Capacity, and the Prospect of Microbiome Manipulation in the Sea Anemone Coral Model Aiptasia

et al. 2021

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“…Attached and free-living communities were dominated by 16 genera (Fig. 2b), many of which are commonly being observed in algal cultures or as epibionts in several phycospheres of dinoflagellates, diatoms, and coccolithophores (Foster et al, 2011;Wang et al, 2016).…”

Section: Composition Of Associated Bacterial Communities and Hydrocar...mentioning

confidence: 99%

Efficiency of benthic diatom-associated bacteria in the removal of benzo(a)pyrene and fluoranthene

Kahla

Garali

Karray

et al. 2021

Science of The Total Environment

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Genomic insights into antioxidant activities of Pyruvatibacter mobilis CGMCC 1.15125T, a pyruvate-requiring bacterium isolated from the marine microalgae culture

Rong

Zheng

et al. 2021

Marine Genomics

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Pyruvatibacter mobilis gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122

Cited by 14 publications

References 27 publications

Surface Topography, Bacterial Carrying Capacity, and the Prospect of Microbiome Manipulation in the Sea Anemone Coral Model Aiptasia

Surface Topography, Bacterial Carrying Capacity, and the Prospect of Microbiome Manipulation in the Sea Anemone Coral Model Aiptasia

Efficiency of benthic diatom-associated bacteria in the removal of benzo(a)pyrene and fluoranthene

Genomic insights into antioxidant activities of Pyruvatibacter mobilis CGMCC 1.15125T, a pyruvate-requiring bacterium isolated from the marine microalgae culture

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