Genomic insights into antioxidant activities of Pyruvatibacter mobilis CGMCC 1.15125T, a pyruvate-requiring bacterium isolated from the marine microalgae culture

Rong, Jin-Cheng; Ji, Bo-Wen; Zheng, Ning; Sun, Zhuo; Li, Yisong; Xie, Bin-Bin

doi:10.1016/j.margen.2020.100791

Cited by 7 publications

(5 citation statements)

References 17 publications

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“…In contrast, an OTU assigned to the genus Pyruvatibacter was more abundant in heat and UVB treated microcosms without HS supplementation. Recently, genomic analysis of a Pyruvatibacter strain isolated from a marine microalga revealed that it could utilize various antioxidants to deal with oxidative stress (Rong et al, 2021). Together with the results of our Fv/Fm analysis, these findings strongly suggest that M. digitata experienced oxidative stress in the microcosms with elevated temperatures and UVB radiation and this was associated with shifts in its bacterial community; HS supplementation, however, appeared to mitigate this.…”

Section: Effects Of Humic Substances Temperature and Uvb On Environme...supporting

confidence: 72%

Humic substances mitigate adverse effects of elevated temperature with potentially critical repercussions for coral reef resilience

Stuij

Cleary

Voogd

et al. 2023

Preprint

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Previous observational studies have suggested that terrestrially-derived compounds, most notably humic substances (HS) can protect coral reefs from thermal stress. No study hitherto has, however, tested this hypothesis. In the present study, we used a randomised-controlled microcosm setup to test to what extent HS are able to mitigate the adverse effects of elevated temperature and intense UVB radiation on coral photosynthetic activity, and environmental and host-associated bacterial ercommunities. Our results clearly demonstrate a significant protective effect of HS. Corals in HS-supplemented microcosms had significantly higher photosynthetic activities than those in microcosms subjected to elevated heat and intense UVB radiation. Our results, furthermore, showed that coral reef organisms in HS-supplemented microcosms contained unique bacterial communities enriched with known groups of potentially beneficial bacteria. Our findings have significant repercussions for reef resilience in the face of increasing climate-induced stressors and highlight the importance of restoring coastal forests and the land-sea interface in order to protect coral reefs.

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Section: Effects Of Humic Substances Temperature and Uvb On Environme...supporting

confidence: 72%

Humic substances mitigate adverse effects of elevated temperature with potentially critical repercussions for coral reef resilience

Stuij

Cleary

Voogd

et al. 2023

Preprint

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“…Muricauda can release Zeaxanthin that improves photosynthetic efficiency and neutralises ROS during temperature stress 16 . Pyruvatibacter requires pyruvate – a well-known ROS scavenger – as a carbon source and some genomes encode genes for antioxidants such as superoxide dismutase and glutathione 80 .…”

Section: Resultsmentioning

confidence: 99%

“…Pyruvatibacter requires pyruvate – a well-known ROS scavenger – as a carbon source and some genomes encode genes for antioxidants such as superoxide dismutase and glutathione 80 .…”

Section: Resultsmentioning

confidence: 99%

Coral endosymbiont growth is enhanced by metabolic interactions with bacteria

Matthews,

Khalil,

Siboni

et al. 2023

Nat Commun

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Bacteria are key contributors to microalgae resource acquisition, competitive performance, and functional diversity, but their potential metabolic interactions with coral microalgal endosymbionts (Symbiodiniaceae) have been largely overlooked. Here, we show that altering the bacterial composition of two widespread Symbiodiniaceae species, during their free-living stage, results in a significant shift in their cellular metabolism. Indeed, the abundance of monosaccharides and the key phytohormone indole-3-acetic acid (IAA) were correlated with the presence of specific bacteria, including members of the Labrenzia (Roseibium) and Marinobacter genera. Single-cell stable isotope tracking revealed that these two bacterial genera are involved in reciprocal exchanges of carbon and nitrogen with Symbiodiniaceae. We identified the provision of IAA by Labrenzia and Marinobacter, and this metabolite caused a significant growth enhancement of Symbiodiniaceae. By unravelling these interkingdom interactions, our work demonstrates how specific bacterial associates fundamentally govern Symbiodiniaceae fitness.

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“…The higher temperature, combined with the availability of Symbiodiniaceae‐derived cellulose (the Symbiodiniaceae cell wall contains large amounts of cellulose; Markell et al, 1992), may have favoured this taxon in SS strains. Pyruvacter mobilis (SS indicator in closely associated fractions) was originally isolated from a green alga and has the genomic potential to produce many antioxidants, such as superoxide dismutase, glutathione, rubrerythrin and globin (Rong et al, 2021). This bacterium may therefore participate in the high ROS‐scavenging abilities of SS strains, which is consistent with the lower net ROS observed in the two SS compared to WT10 under elevated temperature in the acute stress experiment.…”

Section: Discussionmentioning

confidence: 99%

Assessing the contribution of bacteria to the heat tolerance of experimentally evolved coral photosymbionts

Maire,

Deore,

Jameson

et al. 2023

Environmental Microbiology

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Coral reefs are extremely vulnerable to ocean warming, which triggers coral bleaching—the loss of endosymbiotic microalgae (Symbiodiniaceae) from coral tissues, often leading to death. To enhance coral climate resilience, the symbiont, Cladocopium proliferum was experimentally evolved for >10 years under elevated temperatures resulting in increased heat tolerance. Bacterial 16S rRNA gene metabarcoding showed the composition of intra‐ and extracellular bacterial communities of heat‐evolved strains was significantly different from that of wild‐type strains, suggesting bacteria responded to elevated temperatures, and may even play a role in C. proliferum thermal tolerance. To assess whether microbiome transplantation could enhance heat tolerance of the sensitive wild‐type C. proliferum, we transplanted bacterial communities from heat‐evolved to the wild‐type strain and subjected it to acute heat stress. Microbiome transplantation resulted in the incorporation of only 30 low‐abundance strains into the microbiome of wild‐type cultures, while the relative abundance of 14 pre‐existing strains doubled in inoculated versus uninoculated samples. Inoculation with either wild‐type or heat‐evolved bacterial communities boosted C. proliferum growth, although no difference in heat tolerance was observed between the two inoculation treatments. This study provides evidence that Symbiodiniaceae‐associated bacterial communities respond to heat selection and may contribute to coral adaptation to climate change.

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Genomic insights into antioxidant activities of Pyruvatibacter mobilis CGMCC 1.15125T, a pyruvate-requiring bacterium isolated from the marine microalgae culture

Cited by 7 publications

References 17 publications

Humic substances mitigate adverse effects of elevated temperature with potentially critical repercussions for coral reef resilience

Humic substances mitigate adverse effects of elevated temperature with potentially critical repercussions for coral reef resilience

Coral endosymbiont growth is enhanced by metabolic interactions with bacteria

Assessing the contribution of bacteria to the heat tolerance of experimentally evolved coral photosymbionts

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