Pyruvate kinase: modulation by <scp>L</scp>-phenylalanine and <scp>L</scp>-alanine

Vijayvargiya, R; Schwark, Wayne S.; Singhal, Radhey L.

doi:10.1139/o69-140

Cited by 36 publications

(12 citation statements)

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“…10. Similar results were obtained for SGPK-I and SGPK-II and are in agreement with other reports on rabbit muscle (Carminatti et al, 1971) and rat tissues (Vijayvargiya et al, 1969).…”

Section: Alaninesupporting

confidence: 93%

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Biochemical Studies on the Shell Gland of Japanese Quail, Coturnix coturnix japonica

Yamada

1974

Poultry Science

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Pyruvate kinase (EC 2.7.1.40) in Japanese quail shell gland was examined for the multiple components and enzymic properties. Two components were obtained by isoelectric focusing. Isoelectric points of the two components were pH 4.5 (designated SGPK-I) and pH 6.1 (designated SGPK-II), respectively. In the shell gland mucosa, the existence of only SGPK-II was detected. SGPK-I and SGPK-II were eluted from DEAE-cellulose by 0.02M and 0.12M KC1, respectively at pH 7.4 (20mM Tris-HCl). In the absence of fructose-1, 6-diphosphate (FDP), SGPK-I and SGPK-II, showed a sigmoidal response with respect to phosphoenolpyruvate (PEP) concentration, while in the presence of FDP, they both exhibited the Michaelis-Menten type response. Kinetical responses of SGPK-I and SGPK-II were markedly influenced by pH, K + and Mg 2+ concentration even in the presence of FDP. No significant difference in apparent Km value for PEP of the two components was obtained. The inhibition of the two components by ATP was pH-dependent and was reversed by the presence of FDP. The two components from the shell gland were inhibited by Ca 2+ . The inhibition by Ca 2+ was dependent on pH, K + , and Mg 2+ concentration. FDP altered the Km value for PEP several folds smaller, of the two components inhibited by Ca 2+ , but did not change the Vmax. During the egg formation process, the ratio of the summed activity of SGPK-I to that of SGPK-II varied from 0.5 (minimum) to 2.0 (maximum), depending on the position of a developing egg in the oviduct. Physiological significance of the two components in the shell gland is discussed.

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“…10. Similar results were obtained for SGPK-I and SGPK-II and are in agreement with other reports on rabbit muscle (Carminatti et al, 1971) and rat tissues (Vijayvargiya et al, 1969).…”

Section: Alaninesupporting

confidence: 93%

“…Vijayvargiya et al (1969) and Carminatti et al (1971) reported that L-phenylalanine inhibits the pyruvate kinase (M type) by competing with the substrate, PEP. As shown in Fig.…”

Section: « • • •mentioning

confidence: 99%

Biochemical Studies on the Shell Gland of Japanese Quail, Coturnix coturnix japonica

Yamada

1974

Poultry Science

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“…On the other hand, there is no kinetic evidence so far, suggesting that the enzyme from muscle, which has been extensively investigated [2], might have allosteric properties. Recently it was reported that type M pyruvate kinase from skeletal muscle [3] and other tissues [3,4] is inhibited by phenylalanine in a competitive way. It was also found that L-alanine can reverse this inhibition [3].…”

Section: Introductionmentioning

confidence: 99%

“…Recently it was reported that type M pyruvate kinase from skeletal muscle [3] and other tissues [3,4] is inhibited by phenylalanine in a competitive way. It was also found that L-alanine can reverse this inhibition [3].…”

Section: Introductionmentioning

confidence: 99%

Allosteric inhibition of muscle pyruvate kinase by phenylalanine

Asúa

Carminatti

1970

FEBS Letters

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“…Phenylpyruvate has been shown to inhibit a number of brain enzymes (Patel, 1972;Land & Clark, 1973) and phenylalanine inhibits pyruvate kinase from brain (Weber, 1969) and other tissues (Vijayvargiya, Schwark & Singhal, 1969). In the present study, pyruvate kinase and citrate synthetase in rabbit lymphocytes were studied.…”

Section: Discussionmentioning

confidence: 88%

Effect of Phenylalanine and Its Metabolites on the Metabolism of Leucocytes and Lymphocytes

Bradley

Mahler

1975

Clinical Science

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1. The pathogenesis of the mental retardation in phenylketonuria remains obscure. Leucocytes have proved of value in the study of other inborn errors of metabolism. The lymphocyte is a suitable model cell for the study of mammalian metabolism, because of its ability to divide in vitro in response to various stimuli. 2. We have examined the effects of phenylalanine, phenylpyruvate, phenyl-lactate and phenylacetate on the human leucocyte and the resting and phytohaemagglutinin-stimulated rabbit lymphocyte. 3. Phenylpyruvate and phenyl-lactate reduced acetate incorporation into leucocyte lipid by 38% and 48% respectively. Only phenyl-lactate reduced acetate incorporation into the resting and stimulated lymphocyte, by 20% and 34% respectively. 4. Glucose incorporation into leucocyte lipid was unaffected by phenylalanine, phenylpyruvate and phenyl-lactate. Only phenyl-lactate inhibited (46%) the production of CO2 from glucose. 5. Phenylalanine and leucine incorporation into trichloroacetic acid-insoluble material of resting and stimulated lymphocytes was inhibited by phenyl-lactate (10-42%), phenylpyruvate (27-57%) and phenylacetate (19-39%). 6. Uridine incorporation into resting and stimulated cells was inhibited by phenyl-lactate (22-26%), phenylpyruvate (42-52%) and phenylacetate (20%). 7. Thymidine incorporation into resting lymphocytes was reduced by phenyl-lactate, phenylpyruvate, phenylacetate and phenylalanine by 12-26%. Incorporation into the stimulated cell was inhibited by phenylpyruvate and phenyl-lactate (90%) and phenylacetate (66%). 8. Phenylalanine inhibited lymphocyte pyruvate kinase and phenylpyruvate inhibited citrate synthetase. 9. These results are compared with published data relating to experimental hyperphenylalaninaemia and the effects of these metabolites on nervous tissue in vitro.

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Pyruvate kinase: modulation by L-phenylalanine and L-alanine

Cited by 36 publications

References 0 publications

Biochemical Studies on the Shell Gland of Japanese Quail, Coturnix coturnix japonica

Biochemical Studies on the Shell Gland of Japanese Quail, Coturnix coturnix japonica

Allosteric inhibition of muscle pyruvate kinase by phenylalanine

Effect of Phenylalanine and Its Metabolites on the Metabolism of Leucocytes and Lymphocytes

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