Pyruvate dehydrogenase subunit β of Lactobacillus plantarum is a collagen adhesin involved in biofilm formation

Abstract

Multi-functional surface proteins have been observed in a variety of pathogenic bacteria, where they mediate host cell adhesion and invasion, as well as in commensal bacterial species, were they mediate positive interaction with the host. Among these proteins, some glycolytic enzymes, expressed on the bacterial cell surface, can bind human extracellular matrix components (ECM). A major target for them is collagen, an abundant glycoprotein of connective tissues. We have previously shown that the enolase EnoA1 of Lactobacillus plantarum, one of the most predominant species in the gut microbiota of healthy individuals, is involved in binding with collagen type I (CnI). In this study, we found that PDHB, a component of the pyruvate dehydrogenase complex, contributes to the L. plantarum LM3 adhesion to CnI. By a cellular adhesion assay to immobilized CnI, we show that LM3-B1 cells, carrying a null mutation in the pdhB gene, bind to CnI - coated surfaces less efficiently than wild-type cells. Moreover, we show that the PDHB-CnI interaction requires a native state for PDHB. We also analyzed the ability to develop biofilm in wild-type and mutant strains and we found that the lack of the PDHB on cell surface generates cells partially impaired in biofilm development.

Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviae

“…4 and 5). This analysis demonstrates that MSPDHA and MSPDHB are respectively the membrane-associated protein in MS, suggesting that MSPDHA and MSPDHB might possess various biological functions besides the catalytic activity catalyst, such as some membrane-associated proteins have been found in previous studies (Bao et al, 2014;Gao et al, 2018;Salzillo et al, 2017). We suggest that the distribution in the cell membrane fraction of MSPDHA and MSPDHB is consistent with the etiology of adherence to host cells.…”

“…6), and the ELISA revealed that the binding of MSPDHA and MSPDHB to chicken Plg or human Fn in a dose-dependent manner. The PDHB acts as fibronectin and plasminogen-binding protein has already been demonstrated in other microorganisms (Dallo et al, 2002;Salzillo et al, 2017;Thomas et al, 2013), but the binding activity of MSPDHA to chicken Plg and human Fn was first confirmation in this study. Those suggested that MSPDHA and MSPDHB are the major Plg/Fn-.…”

Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components

“…By means of comparative analysis between LM3 and its isogenic LM3-CC1 mutant, it has been demonstrated that EnoA1 is involved in immunostimulation of Caco-2 cells and in biofilm development [ 80 ]. Moreover, the ability of the L. plantarum E1 beta-subunit of pyruvate dehydrogenase (PDHB) to bind to fibronectin and collagen I was as well assessed [ 81 , 82 ]. These studies described for the first time PDHB as an adhesin in a probiotic strain, being previously described as a fibronectin and plasminogen binding adhesin only in the pathogen Mycoplasma pneumoniae [ 83 – 85 ].…”

“…These studies described for the first time PDHB as an adhesin in a probiotic strain, being previously described as a fibronectin and plasminogen binding adhesin only in the pathogen Mycoplasma pneumoniae [ 83 – 85 ]. Similarly to EnoA1 and to other adhesins expressed on cell surface of different commensal or pathogen bacteria, L. plantarum PDHB is also involved in biofilm development [ 82 ]. The identification of lactobacilli adhesins involved in biofilm development, such as EnoA1 and PDHB, is of interest for the possible impact that the biofilm itself may have on persistence of the microorganism in the colon [ 86 ] .…”

pdh modulate virulence through reducing stress tolerance and biofilm formation of Streptococcus suis serotype 2

“…Studies have found that Lactobacillus plantarum pyruvate dehydrogenase (PDHB) is not only involved in biofilm formation but is also a collagen-binding adhesin. Deletion of PDHB results in decreased binding ability of L. plantarum to collagen [24]. We selected porcine renal epithelial cells PK-15 to assess the role of PDH in bacterial adhesion and invasion.…”