Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants

Abstract

Aim Although it is established that peri-implantitis is a bacterially induced disease, little is known about the bacterial profile of peri-implant communities in health and disease. The purpose of the present investigation was to examine the microbial signatures of the peri-implant microbiome in health and disease. Materials and methods Subgingival and submucosal plaque samples were collected from forty subjects with periodontitis, peri-implantitis, periodontal and peri-implant health and analyzed using 16S pyrosequencing. Results Peri-implant biofilms demonstrated significantly lower diversity than subgingival biofilms in both health and disease, however, several species, including previously unsuspected and unknown organisms, were unique to this niche. The predominant species in peri-implant communities belonged to the genera Butyrivibrio, Campylobacter, Eubacterium, Prevotella, Selenomonas, Streptococcus, Actinomyces, Leptotrichia, Propionibacterium, Peptococcus, Lactococcus and Treponema. Peri-implant disease was associated with lower levels of Prevotella and Leptotrichia and higher levels of Actinomyces, Peptococcus, Campylobacter, non-mutans Streptococcus, Butyrivibrio, and Streptococcus mutans than healthy implants. These communities also demonstrated lower levels of Prevotella, non-mutans Streptococcus, Lactobacillus, Selenomonas, Leptotrichia, Actinomyces and higher levels of Peptococcus, Mycoplasma, Eubacterium, Campylobacter, Butyrivibrio, Streptococcus mutans, and Treponema when compared to periodontitis-associated biofilms. Conclusion The peri-implant microbiome differs significantly from the periodontal community in both health and disease. Peri-implantitis is a microbially heterogeneous infection with predominantly gram-negative species, and is less complex than periodontitis.

Leptotrichia species in human infections II

“…Leptotrichia species have been recovered from the blood of patients with liver abscesses, mucositis, neutropenic sepsis, diabetes, respiratory distress, community-acquired pneumonia (CAP), bilateral lung crackles, mild anemia, and vasculitis (Table 2) [7,22,33–35]. They were also recovered from oral plaque of guinea pigs [21] and feces of piglets [54], dental plaque from healthy individuals, plaque and saliva from patients with various types of caries, gingivitis, chronic periodontitis, and peri-implantitis [23,25–27,34,35,37,38,44,45,47,49,52,59,60,62,66–69,91,94], decayed tooth surfaces and discordant caries from intact enamel surfaces [53].…”

Peri-Implant Infections of Oral Biofilm Etiology

“…interdental space, emerging evidence suggests that they could be microbiologically distinct from each other (Kumar et al 2012;Dabdoub et al 2013;Heuer et al 2012). By use of broad-range PCR techniques (Heuer et al 2012) and pyrosequencing (Kumar et al 2012;Dabdoub et al 2013) it was demonstrated that the peri-implant microbiome was distinct from the periodontal microbiome. Given the distinct topography and immunological characteristics of the implant compared to the tooth, the two ecosystems could be regarded divergent.…”

Physiological and microbial adjustments to diet quality permit facultative herbivory in an omnivorous lizard

“…Relative abundances were transformed using a variance stabilizing transformation of arcsin(abundance 0.5 ) (Kumar et al, 2012;Shchipkova et al, 2010). We used JMP 12.0 to compare relative abundances of bacterial phyla and genera using the Response Screening function with the robust fit option to conduct multiple t-tests and correct P-values with the false discovery rate (FDR) correction.…”