Pyrosequencing Analysis of the Apical Root Canal Microbiota

Siqueira, José F.; Alves, Flávio R.F.; Rôças, Isabela N.

doi:10.1016/j.joen.2011.08.012

Cited by 115 publications

(117 citation statements)

References 33 publications

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“…48 More recent approaches included the use of pyrosequencing of 16S rRNA genes to profile the endodontic microbiome. 54,55 These studies revealed a previously unknown high diversity of bacterial communities, also in the apical portion of infected root canals. 55 A comprehensive joint analysis of culture and molecular studies showed that over 460 unique bacterial taxa, belonging to 100 genera and 9 phyla, have been identified in different types of endodontic infections.…”

Section: Culture-independent Studiesmentioning

confidence: 79%

“…54,55 These studies revealed a previously unknown high diversity of bacterial communities, also in the apical portion of infected root canals. 55 A comprehensive joint analysis of culture and molecular studies showed that over 460 unique bacterial taxa, belonging to 100 genera and 9 phyla, have been identified in different types of endodontic infections. 42 The phyla with the highest species richness were confirmed to be Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria.…”

Section: Culture-independent Studiesmentioning

confidence: 79%

See 1 more Smart Citation

On the dynamics of root canal infections—what we understand and what we don't

Zehnder

Belibasakis

2015

Virulence

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Section: Culture-independent Studiesmentioning

confidence: 79%

Section: Culture-independent Studiesmentioning

confidence: 79%

On the dynamics of root canal infections—what we understand and what we don't

Zehnder

Belibasakis

2015

Virulence

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“…Recently, a great interindividual variation in the composition of the apical microbiota has been disclosed by pyrosequencing analyses (28,29).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Endotoxin Levels Found in Primary and Secondary Endodontic Infections

Gomes

Endo

Martinho

2012

Journal of Endodontics

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“…The technology enables a large number of reads in a single run, providing increased sampling depth compared to other techniques (7) and has the major advantage of enabling the detection of low-abundant genera (7,8). So far, only eight studies have used this approach to investigate different types of endodontic infections (7,9–15). From those, only two investigations have examined the endodontic microbiome in teeth with failed endodontic treatment (14,15).…”

mentioning

confidence: 99%

Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing

Tzanetakis

Azcarate‐Peril

Zachaki

et al. 2015

Journal of Endodontics

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Introduction Elucidating the microbial ecology of endodontic infections (EI) is a necessary step in developing effective intra-canal antimicrobials. The aim of the present study was to investigate the bacterial composition of symptomatic and asymptomatic primary and persistent infections in a Greek population, using high throughput sequencing methods. Methods 16S amplicon pyrosequencing of 48 root canal bacterial samples was conducted and sequencing data were analyzed using an oral microbiome-specific (HOMD) and a generic (Greengenes; GG) database. Bacterial abundance and diversity were examined by EI type (primary or persistent) and statistical analysis was performed by using non-parametric and parametric tests accounting for clustered data. Results Bacteroidetes was the most abundant phylum in both infection groups. Significant, albeit weak associations of bacterial diversity were found, as measured by UniFrac distances with infection type (ANOSIM R=0.087, P=0.005) and symptoms (ANOSIM R=0.055, P=0.047). Persistent infections were significantly enriched for Proteobacteria and Tenericutes as compared to primary ones; at the genus level, significant differences were noted for 14 taxa, including increased enrichment of persistent infections for Lactobacillus, Streptococcus, and Sphingomonas. More but less-abundant phyla were identified using the GG database; among those, Cyanobacteria (0.018%) and Acidobacteria (0.007%) were significantly enriched among persistent infections. Persistent infections showed higher Phylogenetic Diversity (asymptomatic: PD=9.2, [standard error (se)=1.3]; symptomatic: PD=8.2, se=0.7) compared to primary infections (asymptomatic: PD=5.9, se=0.8; symptomatic: PD=7.4 se=1.0). Conclusions The present study revealed a high bacterial diversity of EI and suggests that persistent infections may have more diverse bacterial communities than primary infections.

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Pyrosequencing Analysis of the Apical Root Canal Microbiota

Cited by 115 publications

References 33 publications

On the dynamics of root canal infections—what we understand and what we don't

On the dynamics of root canal infections—what we understand and what we don't

Comparison of Endotoxin Levels Found in Primary and Secondary Endodontic Infections

Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing

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