Pyrosequencing analysis of eukaryotic and bacterial communities in faucet biofilms

Liu, Ruyin; Yu, Zhisheng; Guo, Hongguang; Liu, Miaomiao; Zhang, Hongxun; Yang, Min

doi:10.1016/j.scitotenv.2012.07.022

Cited by 83 publications

(70 citation statements)

References 47 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…occurred only in the biofilm samples ( Table 2). This finding was in agreement with previous studies that members of these genera were common potential pathogens in the oligotrophic water supply systems (Giao et al, 2009;Liu et al, 2012a;Vaerewijck et al, 2005), but their abundances were clearly different in the PVC and cast iron biofilms. Mycobacterium spp.…”

Section: Bacterial Communitiessupporting

confidence: 93%

“…However, because the formation of biofilm is a result of successional development into a mature community, that may require several years before a steady state is achieved (Martiny et al, 2003), which limits the relevance of short-term model studies. In our previous research, it was found that the faucet biofilm with about 2 years development still show many characteristics of young biofilm (Liu et al, 2012a). Overall, current information on the microbial community of longterm biofilm within full-scale DSs is scant, especially for biofilm eukaryotic community.…”

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Molecular analysis of long-term biofilm formation on PVC and cast iron surfaces in drinking water distribution system

Liu

Zhu

et al. 2014

Journal of Environmental Sciences

Self Cite

View full text Add to dashboard Cite

a b s t r a c tTo understand the impacts of different plumbing materials on long-term biofilm formation in water supply system, we analyzed microbial community compositions in the bulk water and biofilms on faucets with two different materials-polyvinyl chloride (PVC) and cast iron, which have been frequently used for more than10 years. Pyrosequencing was employed to describe both bacterial and eukaryotic microbial compositions. Bacterial communities in the bulk water and biofilm samples were significantly different from each other. Specific bacterial populations colonized on the surface of different materials. Hyphomicrobia and corrosion associated bacteria, such as Acidithiobacillus spp., Aquabacterium spp., Limnobacter thiooxidans, and Thiocapsa spp., were the most dominant bacteria identified in the PVC and cast iron biofilms, respectively, suggesting that bacterial colonization on the material surfaces was selective. Mycobacteria and Legionella spp. were common potential pathogenic bacteria occurred in the biofilm samples, but their abundance was different in the two biofilm bacterial communities. In contrast, the biofilm samples showed more similar eukaryotic communities than the bulk water. Notably, potential pathogenic fungi, i.e., Aspergillus spp. and Candida parapsilosis, occurred in similar abundance in both biofilms. These results indicated that microbial community, especially bacterial composition was remarkably affected by the different pipe materials (PVC and cast iron).

show abstract

Section: Bacterial Communitiessupporting

confidence: 93%

Section: Introductionmentioning

confidence: 96%

Molecular analysis of long-term biofilm formation on PVC and cast iron surfaces in drinking water distribution system

Liu

Zhu

et al. 2014

Journal of Environmental Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…Rhizobiales, Rhodospirillales and Sphingomonadales are bacterial orders commonly detected in biofilms (Liu et al, 2012;Shaw et al, 2015;Rozej et al, 2015) and the viable sequence counts of these bacterial orders substantially increased during the distribution of treated water from the WTP to homes with piped water or cisterns, and the community fountain (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Bacteria in drinking water sources of a First Nation reserve in Canada

Farenhorst

Jahan

et al. 2017

Science of The Total Environment

View full text Add to dashboard Cite

Approximately 20% of the 600 First Nations reserves across Canada are under a drinking water advisory, often due to unacceptable levels of bacteria. In this study, we detected fecal bacteria at an alarmingly high frequency in drinking water sources in a fly-in First Nations community, most notably in buckets/drums of homes without running water where Escherichia coli levels ranged from 20 to 62,000 CFU/100mL. The water leaving the water treatment plant was free of E. coli and its free residual chlorine concentration (0.67 mg/L) was within the range typically observed for treated water in Canada. Water samples from taps in homes served by cisterns, and those sampled from the water truck and community standpipe, always showed unacceptable levels of E. coli (1 to 2,100 CFU/100mL) and free residual chlorine concentrations below the 0.2 mg/L required to prevent bacterial regrowth. Samples from taps in homes served by piped water had lower levels of E. coli (0 to 2 CFU/100mL). DNA-and RNA-based 16S rRNA Illumina sequencing demonstrated that piped and cisterns water distribution systems showed an abundance of viable cells of Alphaproteobacteria indicative of biofilm formation in pipes and cisterns. The alpha diversity, based on observed OTUs and three other indices, was lowest in water truck samples that supplied water to the cistern and the low free residual chlorine concentration (0.07 mg/L) and predominance of Betaproteobacteria (63% of viable cells) that were immediately detected after the truck had filled up at the water treatment plant was indicative of contamination by particulate matter. Given these findings, First Nation residents living without running water and relying on inadequate water distribution systems are at higher risk of contracting water-born illnesses. We urge all governments in Canada to expand their investments in supporting and sustaining water as a human right in Canada's First Nations communities. 3 GRAPHICAL ABSTRACT

show abstract

“…Among the heterotrophic bacteria potentially supported by the aforementioned primary production, we identified diverse, abundant and prevalent OTUs classified to the family Comamonadaceae (Figures 4a and c). Previous studies identify related organisms predominating in DWDS-associated biofilms (Kalmbach et al, 1999;Kalmbach, 2000), faucet biofilms (Liu et al, 2012) and tap water Abbreviations: ANOSIM, analysis of similarities; DistLM, distance-based linear models; PERMANOVA, permutational analysis of variance.…”

Section: Discussionmentioning

confidence: 99%

Core-satellite populations and seasonality of water meter biofilms in a metropolitan drinking water distribution system

et al. 2015

View full text Add to dashboard Cite

Drinking water distribution systems (DWDSs) harbor the microorganisms in biofilms and suspended communities, yet the diversity and spatiotemporal distribution have been studied mainly in the suspended communities. This study examined the diversity of biofilms in an urban DWDS, its relationship with suspended communities and its dynamics. The studied DWDS in Urbana, Illinois received conventionally treated and disinfected water sourced from the groundwater. Over a 2-year span, biomass were sampled from household water meters (n = 213) and tap water (n = 20) to represent biofilm and suspended communities, respectively. A positive correlation between operational taxonomic unit (OTU) abundance and occupancy was observed. Examined under a 'core-satellite' model, the biofilm community comprised 31 core populations that encompassed 76.7% of total 16 S rRNA gene pyrosequences. The biofilm communities shared with the suspended community highly abundant and prevalent OTUs, which related to methano-/methylotrophs (i.e., Methylophilaceae and Methylococcaceae) and aerobic heterotrophs (Sphingomonadaceae and Comamonadaceae), yet differed by specific core populations and lower diversity and evenness. Multivariate tests indicated seasonality as the main contributor to community structure variation. This pattern was resilient to annual change and correlated to the cyclic fluctuations of core populations. The findings of a distinctive biofilm community assemblage and methano-/methyltrophic primary production provide critical insights for developing more targeted water quality monitoring programs and treatment strategies for groundwater-sourced drinking water systems.

show abstract

Pyrosequencing analysis of eukaryotic and bacterial communities in faucet biofilms

Cited by 83 publications

References 47 publications

Molecular analysis of long-term biofilm formation on PVC and cast iron surfaces in drinking water distribution system

Molecular analysis of long-term biofilm formation on PVC and cast iron surfaces in drinking water distribution system

Bacteria in drinking water sources of a First Nation reserve in Canada

Core-satellite populations and seasonality of water meter biofilms in a metropolitan drinking water distribution system

Contact Info

Product

Resources

About