Pyrogallol from Spirogyra neglecta Inhibits Proliferation and Promotes Apoptosis in Castration-Resistant Prostate Cancer Cells via Modulating Akt/GSK-3β/β-catenin Signaling Pathway

Arjsri, Punnida; Mapoung, Sariya; Semmarath, Warathit; Srisawad, Kamonwan; Tuntiwechapikul, Wirote; Yodkeeree, Supachai; Limtrakul, Pornngarm

doi:10.3390/ijms24076452

Cited by 3 publications

(1 citation statement)

References 66 publications

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“…Previous studies demonstrate that a higher concentration of pyrogallol induces cytotoxicity in liver cancer cell lines like Hep G2 and HUH7 (Ahn et al, 2019). Another study report suggests that pyrogallol could enhance apoptosis by Modulating Akt/GSK-3β/β-catenin signaling Pathway in prostate cancer (Arjsri et al, 2023). In our subsequent study, we examined the effects of pyrogallol on aerobic glycolysis and found substantial results.…”

Section: Discussionmentioning

confidence: 77%

Pyrogallol Suppresses the Tumor Progression via Modulating Aerobic Glycolysis and STAT2 Signaling Pathway in Non-small Cell Lung Carcinoma

Liu,

Chang,

Jin

2024

Pharmacognosy Magazine

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Background: Pyrogallol, a bioactive compound derived from natural sources, is the focus of this study, aiming to investigate its anti-cancer properties against A549 lung cancer cells and uncover the underlying mechanisms. Purpose: This study seeks to elucidate the therapeutic potential of pyrogallol as a novel anti-cancer agent for NSCLC by targeting aerobic glycolysis and signal transducer and activator of transcription 2 (STAT2) signaling pathway. Background: Pyrogallol, a bioactive compound derived from natural sources, is the focus of this study, aiming to investigate its anti-cancer properties against A549 lung cancer cells and uncover the underlying mechanisms. Materials and methods: A range of pyrogallol doses was administered to A549 cells, followed by a comprehensive analysis utilizing bioinformatic tools like Gene Expression Omnibus (GEO), GEO2R, WebGestalt, and the Search Tool for Interactions of Chemicals database. Assessments of cytotoxicity were conducted using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and trypan blue assays post-treatment of A549 cells with pyrogallol. Cell cycle progression and cell death were evaluated via flow cytometry using Annexin V-fluorescein isothiocyanates/propidium iodide double staining. Network analysis helped identify pertinent signaling pathways, while RT-PCR validated mRNA expression changes in A549 cells. Auto dock docking studies were employed to gauge the binding affinity of pyrogallol with targets associated with the STAT2-mediated pathway. Results: Pyrogallol notably impeded the progression of A549 cells by prompting cell cycle arrest in the G0/G1 phase and fostering apoptosis. Network analysis highlighted its role in regulating metabolism, apoptosis, and STAT2 targets linked to lung tumorigenesis. RT-PCR validation affirmed the downregulation of genes associated with cell cycle and apoptosis targets. Furthermore, docking studies indicated a robust binding affinity between pyrogallol and the signaling targets within the STAT2 pathway. Pyrogallol displays the potential to halt the growth of lung cancer cells, indicating its promise as a viable treatment for this condition. Conclusion: These discoveries emphasize the need for additional research and clinical studies to fully harness its therapeutic benefits for lung cancer treatment.

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Section: Discussionmentioning

confidence: 77%

Pyrogallol Suppresses the Tumor Progression via Modulating Aerobic Glycolysis and STAT2 Signaling Pathway in Non-small Cell Lung Carcinoma

Liu,

Chang,

Jin

2024

Pharmacognosy Magazine

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Chemical composition and sensory profile of Spirogyra neglecta (Hassall) Kützing

Sriwattana,

Chokumnoyporn,

Brennan

et al. 2024

Int J of Food Sci Tech

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SummaryThe proximate composition, selected amino acid composition, and chemical constituents of Spirogyra neglecta (Hassall) Kützing, a freshwater algae collected from Phrae province, Thailand was investigated. It was found to be a good source of protein (29.42% dry wt. basis) and fibre (6.70% dry wt. basis). The total amino acid content was 2611.85 mg/100 g edible. The chemical constituents were characterised by GC–MS, which demonstrated that it contained phytol (62.10%), ethyl 5,8,11,14,17‐icosapentaenoate (8.55%) among others. Based on its chemical composition results, it could serve as a potential ingredient with high nutritional values in the food and nutraceutical industry. Furthermore, the descriptive sensory profile consisting of 17 descriptors, namely, green colour, glossy, filamentous, aroma (algae, grassy, fishy, and earthy), flavour (algae, grassy, fishy, and earthy), sweet, bitter, and texture (fibrousness and softness), mouthfeel (entire mouth‐coating), and aftertaste (astringent), were successfully developed to allow characterisation of the sensory quality of this algae.

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Ganoderic Acid A Suppresses Androgenindependent Cell Growth via the AKT/GSK-3β/β-catenin Signaling Pathway

Lyu,

Qu,

Chen

et al. 2024

Pharmacognosy Magazine

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Objective The primary aim of this study is to investigate the specific effects and mechanisms of ganoderic acid A (GAA) on castration-resistant prostate cancer (CRPC). Methods We successfully established an androgen-independent prostate cancer cell line (LNCaP-AI). The LNCaP-AI cells demonstrated accelerated growth, elevated levels of androgen receptor and prostate-specific antigen (PSA), and resistance to the drug enzalutamide. Then, we administered LNCaP-AI cells with GAA or a control and determined the cell growth rate. Next, LNCaP-AI cells with and without GAA treatment were subjected to RNA-seq for mRNA sequencing. The genes were subjected to a Kyoto Encyclopedia of Genes and Genomes analysis. We also checked the expression levels of the targeted genes. Finally, normal LNCaP or LNCaP-AI cells were subcutaneously injected into nude mice to further confirm the effect of GAA. Results Experiments showed that GAA effectively suppressed the growth rate, PSA secretion, migration, and invasion of LNCaP-AI cells. The results of a subsequent RNA-seq analysis identified the PI3K/AKT pathway as the key signaling pathway influenced by GAA. Notably, AKT and GSK-3β phosphorylation was decreased, and β-catenin protein levels were also lowered, as a result of GAA treatment. Moreover, we observed decreased activity in crucial genes, including c-Myc, Cyclin D1, and MMP-2, which play integral roles in CRPC development. Furthermore, GAA inhibited the tumorigenesis of LNCaP-AI cells in nude mice in vivo. Conclusion GAA effectively suppresses the growth of androgen-independent cells by inhibiting the AKT/GSK-3β/β-catenin signaling pathway. These findings demonstrate the potential of GAA as a therapeutic candidate for CRPC.

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Pyrogallol from Spirogyra neglecta Inhibits Proliferation and Promotes Apoptosis in Castration-Resistant Prostate Cancer Cells via Modulating Akt/GSK-3β/β-catenin Signaling Pathway

Cited by 3 publications

References 66 publications

Pyrogallol Suppresses the Tumor Progression via Modulating Aerobic Glycolysis and STAT2 Signaling Pathway in Non-small Cell Lung Carcinoma

Pyrogallol Suppresses the Tumor Progression via Modulating Aerobic Glycolysis and STAT2 Signaling Pathway in Non-small Cell Lung Carcinoma

Chemical composition and sensory profile of Spirogyra neglecta (Hassall) Kützing

Ganoderic Acid A Suppresses Androgenindependent Cell Growth via the AKT/GSK-3β/β-catenin Signaling Pathway

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