Pyridoxal Based Fluorescent Chemosensor for Detection of Copper(II) in Solution With Moderate Selectivity and Live Cell Imaging

Abstract

A pyridoxal-based fluorescent probe HL was synthesized for the detection of Cu(2+) in methanol with moderate selectivity. Upon addition of Cu(2+), to the solution of the probe in methanol exhibited a remarkable change in emission at 500 nm. With the limit of detection of 10 μM, the probe could well meet the recommended (less than 32 μM in drinking water) of the World Health Organization (WHO). The intracellular Cu(2+) imaging behaviour of HL was carried out on HeLa cells.

A highly selective pyridoxal-based chemosensor for the detection of Zn(ii) and application in live-cell imaging; X-ray crystallography of pyridoxal-TRIS Schiff-base Zn(ii) and Cu(ii) complexes

“…[49][50][51] However, only a few reports show pyridoxal-based Schiff-bases act as uorometric or colorimetric chemosensors for the detection of transition metals, biomolecules, and anions. 31,[52][53][54][55][56][57][58][59][60][61][62][63][64] In this report, we explore a pyridoxal-based, Zn(II) selective uorescent probe combined with TRIS that exhibits, ease of synthesis, cost efficiency, chemical and photostability, uorescence selectivity and sensitivity, and fast response time for spectral imaging of live-cells (Scheme 1). The coordination environment for Zn(II) ion complexation is clearly delimited by stoichiometric studies and X-ray crystallography, and the TRIS functionality of the Schiff-base provides alternate hydroxyl binding sites for competing metal cations that are more oxophilic than Zn(II), yielding exceptional selectivity and sensitivity.…”