International audienceAqueous solutions of NADH and of model compounds such as 1-4,dihydro-N1-propyl- and 1-4,dihydro-N1-benzyl-nicotinamide are oxidized on rotating Pt disk electrode at +0.66 ± 0.02 V (N.H.E.). This two-electron wave is pH independent in the range of 7-13. Both macroelectrolysis on Pt at +0.85 V (N.H.E.) and chemical oxidation by [Fe (CN)6]3- lead to NAD+ or model compounds. Differences between biochemical, chemical and electrochemical oxidation of NADH are discussed, especially in terms of energetics and mechanisms of reactions